Restriction map for
pF1KB8317
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# NO CUT ENZYMES ( 131 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflIII AgeI AhdI Alw44I
AlwI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbeI BbrPI BcgI BciVI
BfaI BglII BlnI BsaAI BsaBI
BsaWI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrFI BsrGI BssHII Bst1107I BstEII
BstPI BstXI BstYI BstZ17I Cfr10I
ClaI CpoI DraIII DrdI Eam1105I
Eco47III EcoNI EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HincII HindII
HpaI KasI KpnI MaeI MamI
MfeI MflI MluI MluNI MroI
MscI MslI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SalI SbfI
ScaI SexAI SfcI SfiI SfoI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tsp509I Tth111I XbaI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AccII 1314 CGCG CG^CG
AflII 322 CTTAAG C^TTAAG
AocI 781 CCTCAGG CC^T[ACGT]AGG
BanI 583 GGCACC G^G[CT][GA]CC
BfrI 322 CTTAAG C^TTAAG
BlpI 160 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 160 GCTGAGC GC^T[ACGT]AGC
BsiEI 1334 CGGCCG CG[GA][CT]^CG
BsmBI 928 GAGACG CGTCTC(1/5)
BspMI 621 GCAGGT ACCTGC(4/8)
BssSI 588 CACGAG CACGAG(-5/-1)
BstBI 424 TTCGAA TT^CGAA
BstUI 1314 CGCG CG^CG
Bsu36I 781 CCTCAGG CC^T[ACGT]AGG
CelII 160 GCTGAGC GC^T[ACGT]AGC
DraI 82 TTTAAA TTT^AAA
EagI 1334 CGGCCG C^GGCCG
EclXI 1334 CGGCCG C^GGCCG
Eco52I 1334 CGGCCG C^GGCCG
Eco81I 781 CCTCAGG CC^T[ACGT]AGG
KspI 1313 CCGCGG CCGC^GG
MaeII 607 ACGT A^CGT
MvnI 1314 CGCG CG^CG
NspV 424 TTCGAA TT^CGAA
SacI 1182 GAGCTC GAGCT^C
SacII 1313 CCGCGG CCGC^GG
SapI 990 GAAGAGC GCTCTTC(1/4)
SfuI 424 TTCGAA TT^CGAA
SmaI 923 CCCGGG CCC^GGG
XcmI 725 CCACCCAGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 923 CCCGGG C^CCGGG
XmaI 923 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 566 GACGCC G[GA]^CG[CT]C
1434 GACGCC
AspHI 1093 GAGCAC G[AT]GC[AT]^C
1182 GAGCTC
AvaI 923 CCCGGG C^[CT]CG[GA]G
1177 CCCGAG
BbvCI 700 GCTGAGG CCTCAGC(-5/-2)
769 GCTGAGG
BclI 371 TGATCA T^GATCA
740 TGATCA
BglI 838 GCCGCCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1327 GCCGCCACGGC
BsaHI 566 GACGCC G[GA]^CG[CT]C
1434 GACGCC
BsaI 591 GAGACC GGTCTC(1/5)
892 GAGACC
BsiHKAI 1093 GAGCAC G[AT]GC[AT]^C
1182 GAGCTC
BsoBI 923 CCCGGG C^[CT]CG[GA]G
1177 CCCGAG
BstAPI 829 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1229 GCAGCGTCTGC
DsaI 1313 CCGCGG C^C[GA][CT]GG
1331 CCACGG
EarI 109 GAAGAG CTCTTC(1/4)
990 GAAGAG
FbaI 371 TGATCA T^GATCA
740 TGATCA
HaeII 251 GGCGCT [GA]GCGC^[CT]
1069 AGCGCC
Hin1I 566 GACGCC G[GA]^CG[CT]C
1434 GACGCC
HindIII 227 AAGCTT A^AGCTT
319 AAGCTT
Ksp632I 109 GAAGAG CTCTTC(1/4)
990 GAAGAG
MseI 83 TTAA T^TAA
323 TTAA
PflMI 544 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
729 CCAGGTCATGG
TaqI 425 TCGA T^CGA
941 TCGA
Tru9I 83 TTAA T^TAA
323 TTAA
Tsp45I 266 GTGAC ^GT[GC]AC
1441 GTGAC
TspRI 1142 CACTG CA[GC]TG[ACGT][ACGT]^
1268 CACTG
TthHB8I 425 TCGA T^CGA
941 TCGA
Van91I 544 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
729 CCAGGTCATGG
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# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaII
BanII BbsI BbvI BcnI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrBI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeIII MboI MboII
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI StyI TseI