##################################
# NO CUT ENZYMES (  203 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiWI       BsiYI       
BslI        BsmAI       BsmBI       BsoBI       Bsp1407I    
BspDI       BspEI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstNI       BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      ClaI        CpoI        DpnII       DraI        
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRII      EcoRV       
EcoT22I     FauI        FbaI        FokI        FseI        
FspI        HaeII       HaeIII      HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HpaI        
HphI        KasI        KpnI        Ksp632I     KspI        
MaeI        MaeII       MamI        MboI        MflI        
MluI        MluNI       MroI        MscI        MvaI        
MvnI        NaeI        NarI        NcoI        NdeI        
NdeII       NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        RsaI        RsrII       SacI        SacII       
SalI        SapI        Sau3AI      SbfI        ScaI        
SexAI       SfaNI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TaqI        Tsp45I      Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AspHI         351  GAGCAC               G[AT]GC[AT]^C
AvaII         301  GGTCC                G^G[AT]CC
BcnI          304  CCCGG                CC^[GC]GG
BmyI          351  GAGCAC               G[AGT]GC[ACT]^C
BpmI          356  CTCCAG               CTGGAG(16/14)
BsaJI         207  CCTTGG               C^C[ACGT][ACGT]GG
BsiHKAI       351  GAGCAC               G[AT]GC[AT]^C
BsmFI         302  GTCCC                GGGAC(10/14)
BsmI          240  GAATGC               GAATGC(1/-1)
Bsp1286I      351  GAGCAC               G[AGT]GC[ACT]^C
BspHI         266  TCATGA               T^CATGA
BssKI         304  CCCGG                ^CC[ACGT]GG
Cfr13I        301  GGTCC                G^G[ACGT]CC
DraII         300  GGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoO109I      300  GGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoRI         212  GAATTC               G^AATTC
EcoT14I       207  CCTTGG               C^C[AT][AT]GG
HapII         305  CCGG                 C^CGG
HindIII       181  AAGCTT               A^AGCTT
HpaII         305  CCGG                 C^CGG
MaeIII         39  GTTAC                ^GT[ACGT]AC
MboII          81  TCTTC                GAAGA(8/7)
MfeI          128  CAATTG               C^AATTG
MnlI          377  GAGG                 CCTC(7/6)
MslI          267  CATGACGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          305  CCGG                 C^CGG
MunI          128  CAATTG               C^AATTG
NciI          304  CCCGG                CC^[GC]GG
PleI           55  GACTC                GAGTC(4/5)
PpuMI         300  GGGTCCC              [GA]G^G[AT]CC[CT]
RcaI          266  TCATGA               T^CATGA
Sau96I        301  GGTCC                G^G[ACGT]CC
ScrFI         304  CCCGG                CC^[ACGT]GG
SfcI          155  CTATAG               C^T[GA][CT]AG
StyI          207  CCTTGG               C^C[AT][AT]GG
TspRI         231  CAGTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

BbvI          277  GCAGC                GCAGC(8/12)
              383  GCTGC               
BsrI          230  CCAGT                ACTGG(1/-1)
              358  CCAGT               
Fnu4HI        277  GCAGC                GC^[ACGT]GC
              383  GCTGC               
ItaI          277  GCAGC                GC^[ACGT]GC
              383  GCTGC               
MseI          287  TTAA                 T^TAA
              341  TTAA                
MspA1I        278  CAGCTG               C[AC]G^C[GT]G
              314  CAGCTG              
NlaIII          9  CATG                 CATG^
              267  CATG                
NlaIV         300  GGGTCC               GG[ACGT]^[ACGT]CC
              301  GGTCCC              
PvuII         278  CAGCTG               CAG^CTG
              314  CAGCTG              
TfiI           12  GAATC                G^A[AT]TC
              202  GATTC               
Tru9I         287  TTAA                 T^TAA
              341  TTAA                
TseI          277  GCAGC                G^C[AT]GC
              383  GCTGC               


#########################################
# 3 OR MORE CUT ENZYMES (    7 enzymes) #
#########################################

AcsI        AluI        ApoI        DdeI        HinfI       
MwoI        Tsp509I