################################## # NO CUT ENZYMES ( 82 enzymes) # ################################## AatII Acc65I AclI AcsI AflII AgeI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AvrII BbeI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsiWI BsmBI BspDI BspHI BsrBI Bst1107I BstBI BstEII BstPI BstXI BstZ17I CelII ClaI CpoI DraI EcoNI EcoO65I EcoRI EcoRV EcoT14I FbaI FseI HpaI KasI KpnI MamI MfeI MunI NarI NcoI NdeI NheI NotI NruI NspV PacI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SspI StuI StyI SwaI XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 69 enzymes) # ################################### AccI 1422 GTCGAC GT^[AC][GT]AC AccIII 1077 TCCGGA T^CCGGA AcyI 913 GGCGTC G[GA]^CG[CT]C AhdI 457 GACACGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1239 CCTGAGG CC^T[ACGT]AGG AspEI 457 GACACGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1401 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 81 TGGCCA TGG^CCA BamHI 389 GGATCC G^GATCC BbrPI 1819 CACGTG CAC^GTG BbvCI 1030 GCTGAGG CCTCAGC(-5/-2) BglI 1886 GCCCCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 1810 CTGGAG CTGGAG(16/14) BsaAI 1819 CACGTG [CT]AC^GT[GA] BsaHI 913 GGCGTC G[GA]^CG[CT]C BsaWI 1077 TCCGGA [AT]^CCGG[AT] BseAI 1077 TCCGGA T^CCGGA BsiEI 305 CGGCCG CG[GA][CT]^CG BsmI 11 GCATTC GAATGC(1/-1) Bsp1407I 254 TGTACA T^GTACA BspEI 1077 TCCGGA T^CCGGA BspLU11I 1568 ACATGT A^CATGT BsrFI 151 GCCGGC [GA]^CCGG[CT] BsrGI 254 TGTACA T^GTACA BssHII 126 GCGCGC G^CGCGC BssSI 589 CACGAG CACGAG(-5/-1) Bsu36I 1239 CCTGAGG CC^T[ACGT]AGG Cfr10I 151 GCCGGC [GA]^CCGG[CT] DraII 1846 GGGACCC [GA]G^G[ACGT]CC[CT] EagI 305 CGGCCG C^GGCCG Eam1105I 457 GACACGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 305 CGGCCG C^GGCCG Eco52I 305 CGGCCG C^GGCCG Eco81I 1239 CCTGAGG CC^T[ACGT]AGG EcoO109I 1846 GGGACCC [GA]G^G[ACGT]CC[CT] EcoT22I 1987 ATGCAT ATGCA^T Hin1I 913 GGCGTC G[GA]^CG[CT]C HindIII 900 AAGCTT A^AGCTT MluI 524 ACGCGT A^CGCGT MluNI 81 TGGCCA TGG^CCA MroI 1077 TCCGGA T^CCGGA MscI 81 TGGCCA TGG^CCA MseI 1630 TTAA T^TAA NaeI 151 GCCGGC GCC^GGC NgoMIV 151 GCCGGC G^CCGGC NsiI 1987 ATGCAT ATGCA^T PaeR7I 52 CTCGAG C^TCGAG PflFI 1401 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 618 CCACCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1819 CACGTG CAC^GTG PmlI 1819 CACGTG CAC^GTG PpuMI 1846 GGGACCC [GA]G^G[AT]CC[CT] SacI 1719 GAGCTC GAGCT^C SalI 1422 GTCGAC G^TCGAC SapI 1716 GAAGAGC GCTCTTC(1/4) SbfI 1855 CCTGCAGG CCTGCA^GG SmaI 384 CCCGGG CCC^GGG SmlI 52 CTCGAG C^T[CT][GA]AG SphI 119 GCATGC GCATG^C Sse8387I 1855 CCTGCAGG CCTGCA^GG SspBI 254 TGTACA T^GTACA TfiI 835 GAATC G^A[AT]TC Tru9I 1630 TTAA T^TAA Tsp509I 43 AATT ^AATT Tth111I 1401 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 618 CCACCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 52 CTCGAG C^TCGAG XmaCI 384 CCCGGG C^CCGGG XmaI 384 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AviII 104 TGCGCA TGC^GCA 734 TGCGCA BanII 886 GAGCCC G[GA]GC[CT]^C 1719 GAGCTC BbsI 628 GAAGAC GAAGAC(2/6) 853 GAAGAC BcgI 1008 CGAGCAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 347 GCACGCGCATCG BfaI 748 CTAG C^TAG 1057 CTAG BpuAI 628 GAAGAC GAAGAC(2/6) 853 GAAGAC BseRI 921 CTCCTC GAGGAG(10/8) 841 GAGGAG DdeI 1031 CTGAG C^T[ACGT]AG 1240 CTGAG DraIII 493 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG 1651 CACATGGTG DrdI 907 GACTCGGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1138 GACAAGAGCGTC EaeI 81 TGGCCA [CT]^GGCC[GA] 305 CGGCCG FspI 104 TGCGCA TGC^GCA 734 TGCGCA HgaI 914 GCGTC GACGC(5/10) 1145 GCGTC HincII 1165 GTCAAC GT[CT]^[GA]AC 1422 GTCGAC HindII 1165 GTCAAC GT[CT]^[GA]AC 1422 GTCGAC KspI 415 CCGCGG CCGC^GG 1328 CCGCGG MaeI 748 CTAG C^TAG 1057 CTAG SacII 415 CCGCGG CCGC^GG 1328 CCGCGG ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AccII AciI AfaI AflIII AluI Alw44I AlwI AlwNI Aor51HI ApaLI AspHI AvaI AvaII BanI BbvI BcnI BmyI BsaI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DpnII DsaI EarI Eco47III EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TseI Tsp45I TspRI TthHB8I XhoII