Restriction map for
pF1KB8322
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# NO CUT ENZYMES ( 82 enzymes) #
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AatII Acc65I AclI AcsI AflII
AgeI ApaI ApoI AscI AseI
AsnI Asp700I Asp718I AvrII BbeI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsiWI BsmBI
BspDI BspHI BsrBI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I CelII
ClaI CpoI DraI EcoNI EcoO65I
EcoRI EcoRV EcoT14I FbaI FseI
HpaI KasI KpnI MamI MfeI
MunI NarI NcoI NdeI NheI
NotI NruI NspV PacI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SspI StuI StyI SwaI XbaI
XcmI XmnI
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# ONE CUT ENZYMES ( 69 enzymes) #
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AccI 1422 GTCGAC GT^[AC][GT]AC
AccIII 1077 TCCGGA T^CCGGA
AcyI 913 GGCGTC G[GA]^CG[CT]C
AhdI 457 GACACGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1239 CCTGAGG CC^T[ACGT]AGG
AspEI 457 GACACGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1401 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 81 TGGCCA TGG^CCA
BamHI 389 GGATCC G^GATCC
BbrPI 1819 CACGTG CAC^GTG
BbvCI 1030 GCTGAGG CCTCAGC(-5/-2)
BglI 1886 GCCCCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 1810 CTGGAG CTGGAG(16/14)
BsaAI 1819 CACGTG [CT]AC^GT[GA]
BsaHI 913 GGCGTC G[GA]^CG[CT]C
BsaWI 1077 TCCGGA [AT]^CCGG[AT]
BseAI 1077 TCCGGA T^CCGGA
BsiEI 305 CGGCCG CG[GA][CT]^CG
BsmI 11 GCATTC GAATGC(1/-1)
Bsp1407I 254 TGTACA T^GTACA
BspEI 1077 TCCGGA T^CCGGA
BspLU11I 1568 ACATGT A^CATGT
BsrFI 151 GCCGGC [GA]^CCGG[CT]
BsrGI 254 TGTACA T^GTACA
BssHII 126 GCGCGC G^CGCGC
BssSI 589 CACGAG CACGAG(-5/-1)
Bsu36I 1239 CCTGAGG CC^T[ACGT]AGG
Cfr10I 151 GCCGGC [GA]^CCGG[CT]
DraII 1846 GGGACCC [GA]G^G[ACGT]CC[CT]
EagI 305 CGGCCG C^GGCCG
Eam1105I 457 GACACGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 305 CGGCCG C^GGCCG
Eco52I 305 CGGCCG C^GGCCG
Eco81I 1239 CCTGAGG CC^T[ACGT]AGG
EcoO109I 1846 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT22I 1987 ATGCAT ATGCA^T
Hin1I 913 GGCGTC G[GA]^CG[CT]C
HindIII 900 AAGCTT A^AGCTT
MluI 524 ACGCGT A^CGCGT
MluNI 81 TGGCCA TGG^CCA
MroI 1077 TCCGGA T^CCGGA
MscI 81 TGGCCA TGG^CCA
MseI 1630 TTAA T^TAA
NaeI 151 GCCGGC GCC^GGC
NgoMIV 151 GCCGGC G^CCGGC
NsiI 1987 ATGCAT ATGCA^T
PaeR7I 52 CTCGAG C^TCGAG
PflFI 1401 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 618 CCACCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1819 CACGTG CAC^GTG
PmlI 1819 CACGTG CAC^GTG
PpuMI 1846 GGGACCC [GA]G^G[AT]CC[CT]
SacI 1719 GAGCTC GAGCT^C
SalI 1422 GTCGAC G^TCGAC
SapI 1716 GAAGAGC GCTCTTC(1/4)
SbfI 1855 CCTGCAGG CCTGCA^GG
SmaI 384 CCCGGG CCC^GGG
SmlI 52 CTCGAG C^T[CT][GA]AG
SphI 119 GCATGC GCATG^C
Sse8387I 1855 CCTGCAGG CCTGCA^GG
SspBI 254 TGTACA T^GTACA
TfiI 835 GAATC G^A[AT]TC
Tru9I 1630 TTAA T^TAA
Tsp509I 43 AATT ^AATT
Tth111I 1401 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 618 CCACCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 52 CTCGAG C^TCGAG
XmaCI 384 CCCGGG C^CCGGG
XmaI 384 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AviII 104 TGCGCA TGC^GCA
734 TGCGCA
BanII 886 GAGCCC G[GA]GC[CT]^C
1719 GAGCTC
BbsI 628 GAAGAC GAAGAC(2/6)
853 GAAGAC
BcgI 1008 CGAGCAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
347 GCACGCGCATCG
BfaI 748 CTAG C^TAG
1057 CTAG
BpuAI 628 GAAGAC GAAGAC(2/6)
853 GAAGAC
BseRI 921 CTCCTC GAGGAG(10/8)
841 GAGGAG
DdeI 1031 CTGAG C^T[ACGT]AG
1240 CTGAG
DraIII 493 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
1651 CACATGGTG
DrdI 907 GACTCGGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1138 GACAAGAGCGTC
EaeI 81 TGGCCA [CT]^GGCC[GA]
305 CGGCCG
FspI 104 TGCGCA TGC^GCA
734 TGCGCA
HgaI 914 GCGTC GACGC(5/10)
1145 GCGTC
HincII 1165 GTCAAC GT[CT]^[GA]AC
1422 GTCGAC
HindII 1165 GTCAAC GT[CT]^[GA]AC
1422 GTCGAC
KspI 415 CCGCGG CCGC^GG
1328 CCGCGG
MaeI 748 CTAG C^TAG
1057 CTAG
SacII 415 CCGCGG CCGC^GG
1328 CCGCGG
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# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
Alw44I AlwI AlwNI Aor51HI ApaLI
AspHI AvaI AvaII BanI BbvI
BcnI BmyI BsaI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DpnII
DsaI EarI Eco47III EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TseI
Tsp45I TspRI TthHB8I XhoII