Restriction map for
pF1KB8328
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# NO CUT ENZYMES ( 154 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AcsI AfaI AflII AflIII AgeI
AluI Alw44I AlwI AlwNI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspHI AviII AvrII
BalI BamHI BbrPI BbvCI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BlnI BsaAI BsaBI BsaWI
BseAI BsgI BsiHKAI BsiWI BsmBI
BsmFI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EaeI EagI EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HapII HincII
HindII HindIII HpaI HpaII KpnI
KspI MaeI MamI MfeI MluI
MluNI MroI MscI MseI MspI
MunI MvnI NaeI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuII RcaI RsaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI Tru9I
XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AatII 205 GACGTC GACGT^C
AhdI 322 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 342 CCTCAGG CC^T[ACGT]AGG
ApaI 430 GGGCCC GGGCC^C
AspEI 322 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 118 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 233 CTCGAG C^[CT]CG[GA]G
BbeI 22 GGCGCC GGCGC^C
BbsI 142 GTCTTC GAAGAC(2/6)
BglII 155 AGATCT A^GATCT
BlpI 62 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 62 GCTCAGC GC^T[ACGT]AGC
BpuAI 142 GTCTTC GAAGAC(2/6)
BsaI 136 GAGACC GGTCTC(1/5)
BsiEI 464 CGATCG CG[GA][CT]^CG
BsmAI 136 GAGAC GTCTC(1/5)
BsoBI 233 CTCGAG C^[CT]CG[GA]G
BstYI 155 AGATCT [GA]^GATC[CT]
Bsu36I 342 CCTCAGG CC^T[ACGT]AGG
CelII 62 GCTCAGC GC^T[ACGT]AGC
DraII 430 GGGCCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 322 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 501 GAAGAG CTCTTC(1/4)
Eco81I 342 CCTCAGG CC^T[ACGT]AGG
EcoNI 342 CCTCAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 430 GGGCCCT [GA]G^G[ACGT]CC[CT]
HgaI 118 GACGC GACGC(5/10)
HphI 92 TCACC GGTGA(8/7)
KasI 22 GGCGCC G^GCGCC
Ksp632I 501 GAAGAG CTCTTC(1/4)
MaeII 206 ACGT A^CGT
MaeIII 229 GTCAC ^GT[ACGT]AC
MflI 155 AGATCT [GA]^GATC[CT]
MslI 444 CACTGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 57 CCGCTG C[AC]G^C[GT]G
NarI 22 GGCGCC GG^CGCC
NspI 166 ACATGC [GA]CATG^[CT]
PaeR7I 233 CTCGAG C^TCGAG
PflFI 118 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 328 GAGTC GAGTC(4/5)
PvuI 464 CGATCG CGAT^CG
SapI 501 GAAGAGC GCTCTTC(1/4)
SfaNI 13 GCATC GCATC(5/9)
SfcI 333 CTACAG C^T[GA][CT]AG
SfoI 22 GGCGCC GGC^GCC
SmlI 233 CTCGAG C^T[CT][GA]AG
StuI 71 AGGCCT AGG^CCT
TfiI 314 GATTC G^A[AT]TC
Tsp45I 229 GTCAC ^GT[GC]AC
Tsp509I 221 AATT ^AATT
Tth111I 118 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 443 CCACTGAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 233 CTCGAG C^TCGAG
XhoII 155 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AcyI 22 GGCGCC G[GA]^CG[CT]C
205 GACGTC
BanI 22 GGCGCC G^G[CT][GA]CC
298 GGCACC
BanII 82 GAGCCC G[GA]GC[CT]^C
430 GGGCCC
BpmI 79 CTGGAG CTGGAG(16/14)
267 CTGGAG
BsaHI 22 GGCGCC G[GA]^CG[CT]C
205 GACGTC
BseRI 340 CTCCTC GAGGAG(10/8)
325 GAGGAG
FauI 56 CCCGC CCCGC(4/6)
384 CCCGC
Hin1I 22 GGCGCC G[GA]^CG[CT]C
205 GACGTC
HinfI 314 GATTC G^A[ACGT]TC
328 GAGTC
NlaIII 167 CATG CATG^
189 CATG
PflMI 183 CCAGAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
240 CCAGGATGTGG
TaqI 234 TCGA T^CGA
320 TCGA
TthHB8I 234 TCGA T^CGA
320 TCGA
Van91I 183 CCAGAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
240 CCAGGATGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AvaII BbvI BmyI BsaJI
BsiYI BslI Bsp1286I BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeII
HaeIII HhaI HinP1I ItaI MboI
MboII MnlI MvaI MwoI NdeII
NlaIV Sau3AI Sau96I ScrFI TseI
TspRI