################################## # NO CUT ENZYMES ( 181 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BbvI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI Fnu4HI FokI FseI FspI HaeII HhaI HinP1I HincII HindII HindIII HpaI ItaI KasI KpnI KspI MaeIII MamI MfeI MluI MluNI MroI MscI MseI MslI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AcyI 13 GACGCC G[GA]^CG[CT]C AlwI 60 GATCC GGATC(4/5) AocI 547 CCTGAGG CC^T[ACGT]AGG AspHI 41 GTGCTC G[AT]GC[AT]^C AvaI 423 CCCGGG C^[CT]CG[GA]G BbsI 229 GAAGAC GAAGAC(2/6) BfaI 181 CTAG C^TAG BglII 460 AGATCT A^GATCT BpmI 310 CTCCAG CTGGAG(16/14) BpuAI 229 GAAGAC GAAGAC(2/6) BsaHI 13 GACGCC G[GA]^CG[CT]C BsaI 27 GAGACC GGTCTC(1/5) BsiHKAI 41 GTGCTC G[AT]GC[AT]^C BsmBI 8 GAGACG CGTCTC(1/5) BsoBI 423 CCCGGG C^[CT]CG[GA]G BssSI 454 CACGAG CACGAG(-5/-1) Bsu36I 547 CCTGAGG CC^T[ACGT]AGG Eco81I 547 CCTGAGG CC^T[ACGT]AGG EcoNI 17 CCTTTGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HapII 424 CCGG C^CGG HgaI 13 GACGC GACGC(5/10) Hin1I 13 GACGCC G[GA]^CG[CT]C HpaII 424 CCGG C^CGG HphI 522 GGTGA GGTGA(8/7) MaeI 181 CTAG C^TAG MaeII 285 ACGT A^CGT MspA1I 517 CAGCTG C[AC]G^C[GT]G MspI 424 CCGG C^CGG MwoI 336 GCCCAAGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NspI 483 ACATGC [GA]CATG^[CT] PvuII 517 CAGCTG CAG^CTG SfaNI 385 GCATC GCATC(5/9) SmaI 423 CCCGGG CCC^GGG StuI 68 AGGCCT AGG^CCT TaqI 74 TCGA T^CGA TthHB8I 74 TCGA T^CGA XmaCI 423 CCCGGG C^CCGGG XmaI 423 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AluI 413 AGCT AG^CT 518 AGCT Asp700I 132 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 359 GAAATGATTC AvaII 466 GGACC G^G[AT]CC 491 GGACC BcnI 423 CCCGG CC^[GC]GG 424 CCGGG BmyI 41 GTGCTC G[AGT]GC[ACT]^C 417 GTGCCC BsmAI 8 GAGAC GTCTC(1/5) 27 GAGAC Bsp1286I 41 GTGCTC G[AGT]GC[ACT]^C 417 GTGCCC BstYI 59 AGATCC [GA]^GATC[CT] 460 AGATCT Cac8I 305 GCAGGC GC[ACGT]^[ACGT]GC 345 GCCAGC DsaI 32 CCACGG C^C[GA][CT]GG 267 CCGTGG MflI 59 AGATCC [GA]^GATC[CT] 460 AGATCT NciI 423 CCCGG CC^[GC]GG 424 CCGGG XhoII 59 AGATCC [GA]^GATC[CT] 460 AGATCT XmnI 132 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 359 GAAATGATTC ######################################### # 3 OR MORE CUT ENZYMES ( 25 enzymes) # ######################################### BcgI BsaJI BsiYI BslI BssKI BstNI Cfr13I DdeI DpnII EarI EcoRII HaeIII HinfI Ksp632I MboI MboII MnlI MvaI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TfiI