################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI AocI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BlnI BpuAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspEI BspHI BsrDI BsrGI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I MaeI MamI MfeI MluI MroI MslI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tsp509I Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AflIII 683 ACATGT A^C[GA][CT]GT AlwI 574 GATCC GGATC(4/5) AlwNI 649 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 14 GGGCCC GGGCC^C BalI 2 TGGCCA TGG^CCA BglI 111 GCCTCCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 752 AGATCT A^GATCT BlpI 72 GCTGAGC GC^T[ACGT]AGC Bpu1102I 72 GCTGAGC GC^T[ACGT]AGC BseRI 482 CTCCTC GAGGAG(10/8) BsgI 159 GTGCAG GTGCAG(16/14) BsmAI 557 GAGAC GTCTC(1/5) BspDI 571 ATCGAT AT^CGAT BspLU11I 683 ACATGT A^CATGT BsrBI 549 GAGCGG CCGCTC(-3/-3) BsrFI 380 GCCGGC [GA]^CCGG[CT] BssHII 607 GCGCGC G^CGCGC BssSI 195 CTCGTG CACGAG(-5/-1) BstYI 752 AGATCT [GA]^GATC[CT] CelII 72 GCTGAGC GC^T[ACGT]AGC Cfr10I 380 GCCGGC [GA]^CCGG[CT] ClaI 571 ATCGAT AT^CGAT EcoT14I 173 CCAAGG C^C[AT][AT]GG HinfI 37 GACTC G^A[ACGT]TC KspI 62 CCGCGG CCGC^GG MaeIII 425 GTGAC ^GT[ACGT]AC MboII 783 GAAGA GAAGA(8/7) MflI 752 AGATCT [GA]^GATC[CT] MluNI 2 TGGCCA TGG^CCA MscI 2 TGGCCA TGG^CCA MseI 395 TTAA T^TAA NaeI 380 GCCGGC GCC^GGC NgoMIV 380 GCCGGC G^CCGGC PflMI 648 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 37 GACTC GAGTC(4/5) PpuMI 97 GGGACCC [GA]G^G[AT]CC[CT] SacII 62 CCGCGG CCGC^GG StyI 173 CCAAGG C^C[AT][AT]GG Tru9I 395 TTAA T^TAA Tsp45I 425 GTGAC ^GT[GC]AC Van91I 648 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 752 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AluI 71 AGCT AG^CT 163 AGCT Alw44I 208 GTGCAC G^TGCAC 274 GTGCAC ApaLI 208 GTGCAC G^TGCAC 274 GTGCAC AvaII 98 GGACC G^G[AT]CC 699 GGACC BanII 14 GGGCCC G[GA]GC[CT]^C 337 GAGCCC BsaAI 31 TACGTG [CT]AC^GT[GA] 271 TACGTG BstF5I 66 GGATG GGATG(2/0) 773 GGATG DdeI 73 CTGAG C^T[ACGT]AG 539 CTGAG DpnII 574 GATC ^GATC 753 GATC DsaI 62 CCGCGG C^C[GA][CT]GG 290 CCGTGG FokI 66 GGATG GGATG(9/13) 773 GGATG HgaI 58 GACGC GACGC(5/10) 129 GACGC MaeII 32 ACGT A^CGT 272 ACGT MboI 574 GATC ^GATC 753 GATC NdeII 574 GATC ^GATC 753 GATC NspI 320 ACATGC [GA]CATG^[CT] 683 ACATGT Sau3AI 574 GATC ^GATC 753 GATC TaqI 572 TCGA T^CGA 720 TCGA TspRI 9 CACTG CA[GC]TG[ACGT][ACGT]^ 206 CAGTG TthHB8I 572 TCGA T^CGA 720 TCGA XcmI 8 CCACTGGGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 586 CCAGCAGCCAACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AccII AciI AcyI AfaI AspHI BanI BbeI BbvI BcnI BmyI BpmI BsaHI BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BspMI BsrI BssKI BstNI BstUI Cac8I CfoI Cfr13I DraII EaeI EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII HphI ItaI KasI MnlI MspA1I MspI MvaI MvnI MwoI NarI NciI NlaIII NlaIV RsaI Sau96I ScrFI SfoI TseI