################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII AccI AccII AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AvaII AviII BamHI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BpmI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraII DraIII EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HpaI KspI MamI MluI MroI MvnI MwoI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### Acc65I 388 GGTACC G^GTACC AcyI 235 GGCGCC G[GA]^CG[CT]C Aor51HI 524 AGCGCT AGC^GCT Asp718I 388 GGTACC G^GTACC AvaI 584 CTCGGG C^[CT]CG[GA]G AvrII 538 CCTAGG C^CTAGG BbeI 235 GGCGCC GGCGC^C BcnI 575 CCCGG CC^[GC]GG BfaI 539 CTAG C^TAG BglII 849 AGATCT A^GATCT BlnI 538 CCTAGG C^CTAGG BlpI 604 GCTCAGC GC^T[ACGT]AGC Bpu1102I 604 GCTCAGC GC^T[ACGT]AGC BsaHI 235 GGCGCC G[GA]^CG[CT]C BsmBI 337 GAGACG CGTCTC(1/5) BsoBI 584 CTCGGG C^[CT]CG[GA]G Bsp1407I 179 TGTACA T^GTACA BsrGI 179 TGTACA T^GTACA BstYI 849 AGATCT [GA]^GATC[CT] CelII 604 GCTCAGC GC^T[ACGT]AGC Cfr13I 168 GGGCC G^G[ACGT]CC DrdI 130 GACCCCAACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 894 CCACGG C^C[GA][CT]GG EarI 31 GAAGAG CTCTTC(1/4) Eco47III 524 AGCGCT AGC^GCT EcoT14I 538 CCTAGG C^C[AT][AT]GG HapII 576 CCGG C^CGG Hin1I 235 GGCGCC G[GA]^CG[CT]C HincII 313 GTTGAC GT[CT]^[GA]AC HindII 313 GTTGAC GT[CT]^[GA]AC HindIII 737 AAGCTT A^AGCTT HpaII 576 CCGG C^CGG KasI 235 GGCGCC G^GCGCC KpnI 388 GGTACC GGTAC^C Ksp632I 31 GAAGAG CTCTTC(1/4) MaeI 539 CTAG C^TAG MfeI 530 CAATTG C^AATTG MflI 849 AGATCT [GA]^GATC[CT] MseI 854 TTAA T^TAA MslI 363 CACGGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 576 CCGG C^CGG MunI 530 CAATTG C^AATTG NarI 235 GGCGCC GG^CGCC NciI 575 CCCGG CC^[GC]GG NlaIII 267 CATG CATG^ SacI 878 GAGCTC GAGCT^C Sau96I 168 GGGCC G^G[ACGT]CC ScaI 504 AGTACT AGT^ACT SfoI 235 GGCGCC GGC^GCC SspBI 179 TGTACA T^GTACA StyI 538 CCTAGG C^C[AT][AT]GG Tru9I 854 TTAA T^TAA Tsp45I 492 GTCAC ^GT[GC]AC XhoII 849 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AlwNI 329 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG 407 CAGCTGCTG AspHI 696 GAGCAC G[AT]GC[AT]^C 878 GAGCTC BalI 216 TGGCCA TGG^CCA 708 TGGCCA BbsI 76 GAAGAC GAAGAC(2/6) 148 GTCTTC BpuAI 76 GAAGAC GAAGAC(2/6) 148 GTCTTC BsiHKAI 696 GAGCAC G[AT]GC[AT]^C 878 GAGCTC BsmAI 337 GAGAC GTCTC(1/5) 635 GAGAC CfoI 236 GCGC GCG^C 525 GCGC EaeI 216 TGGCCA [CT]^GGCC[GA] 708 TGGCCA HaeII 235 GGCGCC [GA]GCGC^[CT] 524 AGCGCT HgaI 339 GACGC GACGC(5/10) 624 GCGTC HhaI 236 GCGC GCG^C 525 GCGC HinP1I 236 GCGC G^CGC 525 GCGC MluNI 216 TGGCCA TGG^CCA 708 TGGCCA MscI 216 TGGCCA TGG^CCA 708 TGGCCA PleI 543 GACTC GAGTC(4/5) 582 GACTC SfaNI 307 GATGC GCATC(5/9) 104 GCATC TaqI 834 TCGA T^CGA 876 TCGA Tsp509I 476 AATT ^AATT 531 AATT TthHB8I 834 TCGA T^CGA 876 TCGA XcmI 359 CCAGCACGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 711 CCAGAGTAAAGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AciI AfaI AluI AlwI BanI BanII BbvI BmyI BsaJI BsgI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeII MaeIII MboI MboII MnlI MspA1I MvaI NdeII NlaIV PstI PvuII RsaI Sau3AI ScrFI SfcI TfiI TseI TspRI