Restriction map for
pF1KB8341
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# NO CUT ENZYMES ( 175 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BanI BanII BbeI
BbrPI BbvCI BbvI BcgI BciVI
BclI BcnI BfaI BfrI BglI
BglII BlnI BlpI BmyI Bpu1102I
BsaBI BsaHI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsoBI
Bsp1286I BspDI BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I Cac8I
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT22I FbaI
Fnu4HI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI ItaI KasI KpnI KspI
MaeI MamI MfeI MluI MroI
MspA1I MunI MvnI MwoI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI SwaI
TaqI TseI Tth111I TthHB8I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AciI 1103 GCGG CCGC(-3/-1)
AvaII 69 GGACC G^G[AT]CC
BalI 32 TGGCCA TGG^CCA
BamHI 1733 GGATCC G^GATCC
BsaAI 1451 TACGTG [CT]AC^GT[GA]
BsaI 1164 GAGACC GGTCTC(1/5)
BsaJI 401 CCAAGG C^C[ACGT][ACGT]GG
BsaWI 1240 ACCGGA [AT]^CCGG[AT]
BsiYI 6 CCACTTGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 6 CCACTTGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 83 GGGAC GGGAC(10/14)
BssKI 66 CCTGG ^CC[ACGT]GG
BstNI 66 CCTGG CC^[AT]GG
BstYI 1733 GGATCC [GA]^GATC[CT]
DraII 1693 AGGGCCT [GA]G^G[ACGT]CC[CT]
EaeI 32 TGGCCA [CT]^GGCC[GA]
EcoO109I 1693 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRII 66 CCTGG ^CC[AT]GG
EcoT14I 401 CCAAGG C^C[AT][AT]GG
FauI 1103 GCGGG CCCGC(4/6)
HapII 1241 CCGG C^CGG
HindIII 689 AAGCTT A^AGCTT
HpaII 1241 CCGG C^CGG
MflI 1733 GGATCC [GA]^GATC[CT]
MluNI 32 TGGCCA TGG^CCA
MscI 32 TGGCCA TGG^CCA
MseI 506 TTAA T^TAA
MspI 1241 CCGG C^CGG
MvaI 66 CCTGG CC^[AT]GG
PflMI 6 CCACTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ScrFI 66 CCTGG CC^[ACGT]GG
SfaNI 1725 GCATC GCATC(5/9)
SfcI 114 CTACAG C^T[GA][CT]AG
StyI 401 CCAAGG C^C[AT][AT]GG
Tru9I 506 TTAA T^TAA
Van91I 6 CCACTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1733 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflIII 320 ACATGT A^C[GA][CT]GT
1759 ACATGT
AlwI 1734 GATCC GGATC(4/5)
1733 GGATC
BbsI 1107 GAAGAC GAAGAC(2/6)
1440 GAAGAC
BpuAI 1107 GAAGAC GAAGAC(2/6)
1440 GAAGAC
BsmAI 260 GAGAC GTCTC(1/5)
1164 GAGAC
Bsp1407I 89 TGTACA T^GTACA
413 TGTACA
BspLU11I 320 ACATGT A^CATGT
1759 ACATGT
BsrGI 89 TGTACA T^GTACA
413 TGTACA
BstF5I 27 GGATG GGATG(2/0)
96 GGATG
Cfr13I 69 GGACC G^G[ACGT]CC
1694 GGGCC
EarI 372 CTCTTC CTCTTC(1/4)
800 CTCTTC
FokI 27 GGATG GGATG(9/13)
96 GGATG
HinfI 142 GAATC G^A[ACGT]TC
196 GAATC
Ksp632I 372 CTCTTC CTCTTC(1/4)
800 CTCTTC
MaeII 225 ACGT A^CGT
1452 ACGT
NdeI 749 CATATG CA^TATG
1005 CATATG
NlaIV 69 GGACCC GG[ACGT]^[ACGT]CC
1733 GGATCC
Sau96I 69 GGACC G^G[ACGT]CC
1694 GGGCC
SspBI 89 TGTACA T^GTACA
413 TGTACA
TfiI 142 GAATC G^A[AT]TC
196 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AcsI AfaI AluI ApoI BpmI
BsmI BsrI DdeI DpnII EcoRI
HaeIII HphI MaeIII MboI MboII
MnlI MslI NdeII NlaIII NspI
RsaI Sau3AI StuI Tsp45I Tsp509I
TspRI