##################################
# NO CUT ENZYMES (  192 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BcnI        BfrI        
BglI        BlnI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaHI       BsaI        
BsaWI       BseAI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsmI        Bsp1286I    Bsp1407I    BspDI       
BspEI       BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      ClaI        CpoI        DdeI        DraI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        HpaII       KasI        KpnI        
KspI        MaeII       MaeIII      MfeI        MluI        
MluNI       MroI        MscI        MspA1I      MspI        
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NlaIV       
NotI        NruI        NsiI        NspV        PacI        
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RsrII       
SacI        SacII       SalI        SbfI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       StuI        StyI        SwaI        Tsp45I      
Tth111I     Van91I      XbaI        XcmI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AflIII        101  ACATGT               A^C[GA][CT]GT
AluI          228  AGCT                 AG^CT
AlwI            7  GATCC                GGATC(4/5)
AvaI          230  CTCGAG               C^[CT]CG[GA]G
AvaII         252  GGACC                G^G[AT]CC
BbvI           17  GCAGC                GCAGC(8/12)
BfaI           26  CTAG                 C^TAG
BsaBI          79  GATGACAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI         358  CCTGGG               C^C[ACGT][ACGT]GG
BseRI         145  GAGGAG               GAGGAG(10/8)
BsoBI         230  CTCGAG               C^[CT]CG[GA]G
BspHI          72  TCATGA               T^CATGA
BspLU11I      101  ACATGT               A^CATGT
BsrI          267  CCAGT                ACTGG(1/-1)
BssKI         358  CCTGG                ^CC[ACGT]GG
BstF5I        107  GGATG                GGATG(2/0)
BstNI         358  CCTGG                CC^[AT]GG
Cfr13I        252  GGACC                G^G[ACGT]CC
EcoRI         124  GAATTC               G^AATTC
EcoRII        358  CCTGG                ^CC[AT]GG
Fnu4HI         17  GCAGC                GC^[ACGT]GC
FokI          107  GGATG                GGATG(9/13)
HinfI         333  GAATC                G^A[ACGT]TC
HphI          363  GGTGA                GGTGA(8/7)
ItaI           17  GCAGC                GC^[ACGT]GC
MaeI           26  CTAG                 C^TAG
MamI           79  GATGACAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvaI          358  CCTGG                CC^[AT]GG
MwoI           20  GCAGTACTAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI          101  ACATGT               [GA]CATG^[CT]
PaeR7I        230  CTCGAG               C^TCGAG
RcaI           72  TCATGA               T^CATGA
Sau96I        252  GGACC                G^G[ACGT]CC
ScaI           22  AGTACT               AGT^ACT
ScrFI         358  CCTGG                CC^[ACGT]GG
SfaNI          35  GCATC                GCATC(5/9)
SmlI          230  CTCGAG               C^T[CT][GA]AG
SspI          376  AATATT               AAT^ATT
TaqI          231  TCGA                 T^CGA
TfiI          333  GAATC                G^A[AT]TC
TseI           17  GCAGC                G^C[AT]GC
TspRI         288  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       231  TCGA                 T^CGA
XhoI          230  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

AcsI          124  GAATTC               [GA]^AATT[CT]
              163  GAATTT              
ApoI          124  GAATTC               [GA]^AATT[CT]
              163  GAATTT              
BglII         234  AGATCT               A^GATCT
              309  AGATCT              
MnlI          145  GAGG                 CCTC(7/6)
              199  GAGG                
MseI           59  TTAA                 T^TAA
              272  TTAA                
SapI          205  GAAGAGC              GCTCTTC(1/4)
              296  GCTCTTC             
Tru9I          59  TTAA                 T^TAA
              272  TTAA                
Tsp509I       125  AATT                 ^AATT
              164  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   14 enzymes) #
#########################################

AfaI        BstYI       DpnII       EarI        Ksp632I     
MboI        MboII       MflI        MslI        NdeII       
NlaIII      RsaI        Sau3AI      XhoII