################################## # NO CUT ENZYMES ( 124 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI AlwI ApaI ApoI AscI AseI AsnI Asp718I AspEI AvrII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlnI BsaAI BsaBI BsaI BsaWI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I Cfr10I ClaI CpoI DraI DrdI DsaI EagI Eam1105I EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KasI KpnI KspI MaeIII MamI MfeI MflI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I RcaI RsrII SacI SacII SalI SbfI ScaI SfoI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TfiI Tsp45I XbaI XhoI XhoII ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccI 889 GTCTAC GT^[AC][GT]AC AccII 163 CGCG CG^CG AcyI 955 GACGCC G[GA]^CG[CT]C Alw44I 608 GTGCAC G^TGCAC AocI 904 CCTGAGG CC^T[ACGT]AGG Aor51HI 1001 AGCGCT AGC^GCT ApaLI 608 GTGCAC G^TGCAC AspHI 608 GTGCAC G[AT]GC[AT]^C BalI 665 TGGCCA TGG^CCA BfaI 589 CTAG C^TAG BlpI 354 GCTCAGC GC^T[ACGT]AGC BmyI 608 GTGCAC G[AGT]GC[ACT]^C Bpu1102I 354 GCTCAGC GC^T[ACGT]AGC BsaHI 955 GACGCC G[GA]^CG[CT]C BsgI 940 GTGCAG GTGCAG(16/14) BsiEI 732 CGATCG CG[GA][CT]^CG BsiHKAI 608 GTGCAC G[AT]GC[AT]^C BsmBI 52 GAGACG CGTCTC(1/5) Bsp1286I 608 GTGCAC G[AGT]GC[ACT]^C BsrBI 283 GAGCGG CCGCTC(-3/-3) BstAPI 942 GCAGATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 163 CGCG CG^CG BstXI 560 CCAATTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 904 CCTGAGG CC^T[ACGT]AGG CelII 354 GCTCAGC GC^T[ACGT]AGC DraIII 538 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 1001 AGCGCT AGC^GCT Eco81I 904 CCTGAGG CC^T[ACGT]AGG EcoNI 900 CCTCCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 1052 CCAAGG C^C[AT][AT]GG FauI 789 GCGGG CCCGC(4/6) Hin1I 955 GACGCC G[GA]^CG[CT]C HphI 426 GGTGA GGTGA(8/7) MaeI 589 CTAG C^TAG MluNI 665 TGGCCA TGG^CCA MscI 665 TGGCCA TGG^CCA MseI 722 TTAA T^TAA MslI 27 CATTGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 163 CGCG CG^CG PflMI 738 CCAGTACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 636 GACAATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 949 CTGCAG CTGCA^G PvuI 732 CGATCG CGAT^CG PvuII 268 CAGCTG CAG^CTG SapI 327 GAAGAGC GCTCTTC(1/4) StyI 1052 CCAAGG C^C[AT][AT]GG Tru9I 722 TTAA T^TAA TspRI 1128 CAGTG CA[GC]TG[ACGT][ACGT]^ Van91I 738 CCAGTACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 959 CCATCAGCAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### Asp700I 918 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1093 GAAGCTGTTC AspI 529 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC 877 GACCCCGTC BbsI 46 GAAGAC GAAGAC(2/6) 402 GAAGAC BglI 863 GCCCCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1042 GCCAGCGAGGC BpuAI 46 GAAGAC GAAGAC(2/6) 402 GAAGAC BspMI 621 GCAGGT ACCTGC(4/8) 1069 GCAGGT BstF5I 504 GGATG GGATG(2/0) 1122 GGATG DpnII 474 GATC ^GATC 733 GATC EarI 82 CTCTTC CTCTTC(1/4) 327 GAAGAG FokI 504 GGATG GGATG(9/13) 1122 GGATG HaeII 245 AGCGCC [GA]GCGC^[CT] 1001 AGCGCT HinfI 415 GACTC G^A[ACGT]TC 895 GAGTC Ksp632I 82 CTCTTC CTCTTC(1/4) 327 GAAGAG MboI 474 GATC ^GATC 733 GATC NdeII 474 GATC ^GATC 733 GATC PflFI 529 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC 877 GACCCCGTC PleI 415 GACTC GAGTC(4/5) 895 GAGTC Sau3AI 474 GATC ^GATC 733 GATC SexAI 539 ACCTGGT A^CC[AT]GGT 566 ACCTGGT SfcI 9 CTACAG C^T[GA][CT]AG 949 CTGCAG SfiI 862 GGCCCCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 1041 GGCCAGCGAGGCC SmaI 104 CCCGGG CCC^GGG 689 CCCGGG SphI 332 GCATGC GCATG^C 488 GCATGC Tsp509I 562 AATT ^AATT 650 AATT Tth111I 529 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC 877 GACCCCGTC XmaCI 104 CCCGGG C^CCGGG 689 CCCGGG XmaI 104 CCCGGG C^CCGGG 689 CCCGGG XmnI 918 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1093 GAAGCTGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 56 enzymes) # ######################################### AciI AfaI AluI AlwNI AvaI AvaII AviII BbvI BcnI BpmI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI BsrI BssKI BstNI Cac8I CfoI Cfr13I DdeI DraII EaeI EcoO109I EcoRII Fnu4HI FspI HaeIII HapII HgaI HhaI HinP1I HpaII ItaI MaeII MboII MnlI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV NspI PpuMI RsaI Sau96I ScrFI SfaNI TaqI TseI TthHB8I