Restriction map for
pF1KB8358
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BpuAI BsaAI BsaWI BseAI BsiHKAI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstYI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraIII
Eam1105I EarI Eco47III Eco81I EcoO65I
EcoRV FbaI FseI FspI HindIII
HpaI KasI KpnI Ksp632I MfeI
MflI MluI MluNI MroI MscI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccI 235 GTCTAC GT^[AC][GT]AC
AccII 340 CGCG CG^CG
AcsI 406 GAATTC [GA]^AATT[CT]
AlwI 17 GGATC GGATC(4/5)
ApaI 444 GGGCCC GGGCC^C
ApoI 406 GAATTC [GA]^AATT[CT]
AspI 229 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BfaI 712 CTAG C^TAG
BglI 916 GCCAGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 198 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 198 GCTGAGC GC^T[ACGT]AGC
BsaBI 18 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 142 GAGACC GGTCTC(1/5)
BsgI 172 GTGCAG GTGCAG(16/14)
BsiEI 190 CGGCCG CG[GA][CT]^CG
BsmAI 142 GAGAC GTCTC(1/5)
BsmI 105 GCATTC GAATGC(1/-1)
BstAPI 116 GCAAAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 340 CGCG CG^CG
BstXI 86 CCACTATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 198 GCTGAGC GC^T[ACGT]AGC
DpnII 18 GATC ^GATC
DraI 259 TTTAAA TTT^AAA
DrdI 169 GACGTGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 190 CGGCCG [CT]^GGCC[GA]
EagI 190 CGGCCG C^GGCCG
EclXI 190 CGGCCG C^GGCCG
Eco52I 190 CGGCCG C^GGCCG
EcoNI 306 CCTGACGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 406 GAATTC G^AATTC
HaeII 1009 GGCGCT [GA]GCGC^[CT]
HincII 1003 GTCAAC GT[CT]^[GA]AC
HindII 1003 GTCAAC GT[CT]^[GA]AC
HinfI 769 GAGTC G^A[ACGT]TC
HphI 707 TCACC GGTGA(8/7)
KspI 339 CCGCGG CCGC^GG
MaeI 712 CTAG C^TAG
MaeII 170 ACGT A^CGT
MaeIII 167 GTGAC ^GT[ACGT]AC
MamI 18 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 18 GATC ^GATC
MboII 438 GAAGA GAAGA(8/7)
MvnI 340 CGCG CG^CG
NdeII 18 GATC ^GATC
PflFI 229 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 844 CCATCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 769 GAGTC GAGTC(4/5)
PpuMI 314 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 309 GACGGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 961 CTGCAG CTGCA^G
PvuII 932 CAGCTG CAG^CTG
SacII 339 CCGCGG CCGC^GG
Sau3AI 18 GATC ^GATC
SfcI 961 CTGCAG C^T[GA][CT]AG
SmlI 673 CTCAAG C^T[CT][GA]AG
Tsp45I 167 GTGAC ^GT[GC]AC
Tth111I 229 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 844 CCATCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 83 CCACCACTATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AcyI 79 GACGCC G[GA]^CG[CT]C
756 GGCGTC
BanII 368 GGGCTC G[GA]GC[CT]^C
444 GGGCCC
BcnI 188 CCCGG CC^[GC]GG
429 CCGGG
BsaHI 79 GACGCC G[GA]^CG[CT]C
756 GGCGTC
BseRI 816 CTCCTC GAGGAG(10/8)
819 CTCCTC
BspMI 121 ACCTGC ACCTGC(4/8)
174 GCAGGT
BsrI 860 CCAGT ACTGG(1/-1)
917 CCAGT
DdeI 199 CTGAG C^T[ACGT]AG
373 CTGAG
EcoT14I 849 CCATGG C^C[AT][AT]GG
867 CCATGG
EcoT22I 1 ATGCAT ATGCA^T
523 ATGCAT
HapII 189 CCGG C^CGG
429 CCGG
HgaI 79 GACGC GACGC(5/10)
757 GCGTC
Hin1I 79 GACGCC G[GA]^CG[CT]C
756 GGCGTC
HpaII 189 CCGG C^CGG
429 CCGG
MseI 260 TTAA T^TAA
1027 TTAA
MslI 87 CACTATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
618 CACCCTCATG
MspI 189 CCGG C^CGG
429 CCGG
NciI 188 CCCGG CC^[GC]GG
429 CCGGG
NcoI 849 CCATGG C^CATGG
867 CCATGG
NsiI 1 ATGCAT ATGCA^T
523 ATGCAT
StyI 849 CCATGG C^C[AT][AT]GG
867 CCATGG
Tru9I 260 TTAA T^TAA
1027 TTAA
Tsp509I 242 AATT ^AATT
407 AATT
TspRI 602 CACTG CA[GC]TG[ACGT][ACGT]^
918 CAGTG
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AfaI AluI AvaII BanI
BbvI BmyI BpmI BsaJI BsiYI
BslI BsmFI Bsp1286I BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DraII
DsaI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HhaI HinP1I ItaI
MnlI MspA1I MvaI MwoI NlaIII
NlaIV RsaI Sau96I ScrFI SfaNI
TaqI TseI TthHB8I