##################################
# NO CUT ENZYMES (  191 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BanI        BbeI        BbrPI       BbvCI       BclI        
BcnI        BfaI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmFI       BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstF5I      BstPI       BstXI       BstZ17I     
Bsu36I      CelII       Cfr10I      Cfr13I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        FokI        FseI        
FspI        HaeII       HapII       Hin1I       HincII      
HindII      HindIII     HpaI        HpaII       KasI        
KpnI        Ksp632I     KspI        MaeI        MaeII       
MamI        MfeI        MluI        MluNI       MroI        
MscI        MseI        MslI        MspI        MunI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NlaIV       NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        Sau96I      
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StyI        SwaI        Tru9I       Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AccII         288  CGCG                 CG^CG
AciI          289  GCGG                 CCGC(-3/-1)
AcsI           34  AAATTT               [GA]^AATT[CT]
AfaI           15  GTAC                 GT^AC
AlwI          258  GATCC                GGATC(4/5)
ApoI           34  AAATTT               [GA]^AATT[CT]
BanII         371  GAGCCC               G[GA]GC[CT]^C
BcgI           21  GCAGCAGCGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI          81  GGATAC               GTATCC(6/5)
BglI           46  GCCTTCTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI          215  CTGGAG               CTGGAG(16/14)
BsaJI         314  CCTGGG               C^C[ACGT][ACGT]GG
BsgI          376  CTGCAC               GTGCAG(16/14)
BsmAI         179  GAGAC                GTCTC(1/5)
BstUI         288  CGCG                 CG^CG
BstYI         257  AGATCC               [GA]^GATC[CT]
CfoI          287  GCGC                 GCG^C
DpnII         258  GATC                 ^GATC
HaeIII        312  GGCC                 GG^CC
HgaI           27  GCGTC                GACGC(5/10)
HhaI          287  GCGC                 GCG^C
HinP1I        287  GCGC                 G^CGC
HinfI         454  GAATC                G^A[ACGT]TC
HphI          404  TCACC                GGTGA(8/7)
MboI          258  GATC                 ^GATC
MflI          257  AGATCC               [GA]^GATC[CT]
MvnI          288  CGCG                 CG^CG
NdeII         258  GATC                 ^GATC
NlaIII        237  CATG                 CATG^
RsaI           15  GTAC                 GT^AC
Sau3AI        258  GATC                 ^GATC
SfaNI         495  GATGC                GCATC(5/9)
SmlI          446  CTCAAG               C^T[CT][GA]AG
StuI          311  AGGCCT               AGG^CCT
TaqI           30  TCGA                 T^CGA
TfiI          454  GAATC                G^A[AT]TC
TthHB8I        30  TCGA                 T^CGA
XhoII         257  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

BsmI          388  GAATGC               GAATGC(1/-1)
               55  GCATTC              
BssKI         309  CCAGG                ^CC[ACGT]GG
              314  CCTGG               
BstNI         309  CCAGG                CC^[AT]GG
              314  CCTGG               
DdeI          221  CTGAG                C^T[ACGT]AG
              458  CTAAG               
EcoRII        309  CCAGG                ^CC[AT]GG
              314  CCTGG               
MaeIII        326  GTGAC                ^GT[ACGT]AC
              403  GTCAC               
MnlI          445  CCTC                 CCTC(7/6)
              202  GAGG                
MspA1I         41  CAGCTG               C[AC]G^C[GT]G
               74  CAGCTG              
MvaI          309  CCAGG                CC^[AT]GG
              314  CCTGG               
PvuII          41  CAGCTG               CAG^CTG
               74  CAGCTG              
ScrFI         309  CCAGG                CC^[ACGT]GG
              314  CCTGG               
Tsp45I        326  GTGAC                ^GT[GC]AC
              403  GTCAC               
Tsp509I        35  AATT                 ^AATT
              281  AATT                
TspRI         151  CAGTG                CA[GC]TG[ACGT][ACGT]^
              174  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   15 enzymes) #
#########################################

AluI        BbsI        BbvI        BmyI        BpuAI       
BsiYI       BslI        Bsp1286I    BsrI        Cac8I       
Fnu4HI      ItaI        MboII       MwoI        TseI