Restriction map for
pF1KB8361
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# NO CUT ENZYMES ( 184 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaII
AviII AvrII BalI BanII BbeI
BbrPI BbvCI BcgI BciVI BcnI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmAI
BsmBI BsmFI BsmI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssKI BssSI
Bst1107I BstAPI BstBI BstEII BstNI
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoRII EcoRV EcoT22I FauI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HindIII HpaI HpaII
KasI KpnI Ksp632I KspI MfeI
MluI MluNI MroI MscI MseI
MslI MspA1I MspI MunI MvaI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScrFI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI Tru9I
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AciI 110 CCGC CCGC(-3/-1)
AluI 253 AGCT AG^CT
AspHI 417 GAGCAC G[AT]GC[AT]^C
AvaI 270 CTCGGG C^[CT]CG[GA]G
BamHI 160 GGATCC G^GATCC
BanI 238 GGCACC G^G[CT][GA]CC
BbsI 3 GAAGAC GAAGAC(2/6)
BfaI 356 CTAG C^TAG
BglI 137 GCCACTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 417 GAGCAC G[AGT]GC[ACT]^C
BpmI 220 CTGGAG CTGGAG(16/14)
BpuAI 3 GAAGAC GAAGAC(2/6)
BsaBI 27 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 417 GAGCAC G[AT]GC[AT]^C
BsoBI 270 CTCGGG C^[CT]CG[GA]G
Bsp1286I 417 GAGCAC G[AGT]GC[ACT]^C
BsrI 387 CCAGT ACTGG(1/-1)
BstYI 160 GGATCC [GA]^GATC[CT]
Cac8I 48 GCAGGC GC[ACGT]^[ACGT]GC
Cfr13I 51 GGCCC G^G[ACGT]CC
DsaI 54 CCATGG C^C[GA][CT]GG
EcoNI 42 CCTACTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
MaeI 356 CTAG C^TAG
MaeIII 193 GTCAC ^GT[ACGT]AC
MamI 27 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 3 GAAGA GAAGA(8/7)
MflI 160 GGATCC [GA]^GATC[CT]
NcoI 54 CCATGG C^CATGG
PleI 301 GACTC GAGTC(4/5)
Sau96I 51 GGCCC G^G[ACGT]CC
ScaI 389 AGTACT AGT^ACT
SfaNI 172 GATGC GCATC(5/9)
TfiI 281 GAATC G^A[AT]TC
Tsp45I 193 GTCAC ^GT[GC]AC
XhoII 160 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 384 GTAC GT^AC
390 GTAC
BclI 26 TGATCA T^GATCA
83 TGATCA
BsaJI 54 CCATGG C^C[ACGT][ACGT]GG
167 CCAAGG
BsiYI 12 CCTACTGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
42 CCTACTGCAGG
BslI 12 CCTACTGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
42 CCTACTGCAGG
BspHI 29 TCATGA T^CATGA
198 TCATGA
BstF5I 171 GGATG GGATG(2/0)
232 GGATG
DdeI 115 CTCAG C^T[ACGT]AG
250 CTGAG
EcoT14I 54 CCATGG C^C[AT][AT]GG
167 CCAAGG
FbaI 26 TGATCA T^GATCA
83 TGATCA
FokI 171 GGATG GGATG(9/13)
232 GGATG
HaeIII 40 GGCC GG^CC
51 GGCC
HincII 90 GTTGAC GT[CT]^[GA]AC
314 GTCAAC
HindII 90 GTTGAC GT[CT]^[GA]AC
314 GTCAAC
HinfI 281 GAATC G^A[ACGT]TC
301 GACTC
HphI 66 GGTGA GGTGA(8/7)
284 TCACC
MaeII 216 ACGT A^CGT
228 ACGT
NlaIV 160 GGATCC GG[ACGT]^[ACGT]CC
238 GGCACC
RcaI 29 TCATGA T^CATGA
198 TCATGA
RsaI 384 GTAC GT^AC
390 GTAC
StyI 54 CCATGG C^C[AT][AT]GG
167 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AcsI AlwI ApoI BbvI DpnII
Fnu4HI ItaI MboI MnlI MwoI
NdeII NlaIII PstI Sau3AI SfcI
TseI Tsp509I TspRI