################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccIII AclI AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspI AviII AvrII BalI BbrPI BbvCI BcgI BciVI BclI BfaI BglI BglII BlnI BsaAI BsaBI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI DraI DraII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FbaI FseI FspI HindIII HpaI KpnI Ksp632I MaeI MamI MfeI MluI MluNI MroI MscI MunI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI PvuII RcaI RsrII SapI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tth111I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 67 enzymes) # ################################### AccI 145 GTCGAC GT^[AC][GT]AC AcsI 13 GAATTC [GA]^AATT[CT] AcyI 343 GGCGCC G[GA]^CG[CT]C AfaI 120 GTAC GT^AC AflII 417 CTTAAG C^TTAAG ApoI 13 GAATTC [GA]^AATT[CT] AseI 478 ATTAAT AT^TAAT AsnI 478 ATTAAT AT^TAAT AvaI 282 CCCGGG C^[CT]CG[GA]G AvaII 360 GGACC G^G[AT]CC BamHI 408 GGATCC G^GATCC BanI 343 GGCGCC G^G[CT][GA]CC BanII 298 GAGCTC G[GA]GC[CT]^C BbeI 343 GGCGCC GGCGC^C BbsI 33 GAAGAC GAAGAC(2/6) BfrI 417 CTTAAG C^TTAAG BlpI 78 GCTCAGC GC^T[ACGT]AGC Bpu1102I 78 GCTCAGC GC^T[ACGT]AGC BpuAI 33 GAAGAC GAAGAC(2/6) BsaHI 343 GGCGCC G[GA]^CG[CT]C BsaI 313 GAGACC GGTCTC(1/5) BsgI 364 CTGCAC GTGCAG(16/14) BsmAI 313 GAGAC GTCTC(1/5) BsoBI 282 CCCGGG C^[CT]CG[GA]G BspMI 362 ACCTGC ACCTGC(4/8) BsrFI 287 GCCGGC [GA]^CCGG[CT] BsrI 472 CCAGT ACTGG(1/-1) BstAPI 366 GCACTGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 78 GCTCAGC GC^T[ACGT]AGC Cfr10I 287 GCCGGC [GA]^CCGG[CT] DraIII 367 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 329 CCGCGG C^C[GA][CT]GG EaeI 128 CGGCCA [CT]^GGCC[GA] EcoNI 228 CCTGATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 13 GAATTC G^AATTC FauI 328 CCCGC CCCGC(4/6) HaeII 343 GGCGCC [GA]GCGC^[CT] Hin1I 343 GGCGCC G[GA]^CG[CT]C KasI 343 GGCGCC G^GCGCC KspI 329 CCGCGG CCGC^GG MaeIII 379 GTGAC ^GT[ACGT]AC MboII 33 GAAGA GAAGA(8/7) MslI 395 CATCTCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 329 CCGCGG C[AC]G^C[GT]G NaeI 287 GCCGGC GCC^GGC NarI 343 GGCGCC GG^CGCC NgoMIV 287 GCCGGC G^CCGGC NlaIII 456 CATG CATG^ PleI 93 GAGTC GAGTC(4/5) PshBI 478 ATTAAT AT^TAAT PstI 211 CTGCAG CTGCA^G RsaI 120 GTAC GT^AC SacI 298 GAGCTC GAGCT^C SacII 329 CCGCGG CCGC^GG SalI 145 GTCGAC G^TCGAC SfaNI 61 GATGC GCATC(5/9) SfcI 211 CTGCAG C^T[GA][CT]AG SfoI 343 GGCGCC GGC^GCC SmaI 282 CCCGGG CCC^GGG SmlI 417 CTTAAG C^T[CT][GA]AG TaqI 146 TCGA T^CGA TfiI 384 GAATC G^A[AT]TC Tsp45I 379 GTGAC ^GT[GC]AC Tsp509I 14 AATT ^AATT TthHB8I 146 TCGA T^CGA XmaCI 282 CCCGGG C^CCGGG XmaI 282 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccII 271 CGCG CG^CG 330 CGCG AluI 134 AGCT AG^CT 299 AGCT AspHI 76 GTGCTC G[AT]GC[AT]^C 298 GAGCTC BmyI 76 GTGCTC G[AGT]GC[ACT]^C 298 GAGCTC BpmI 23 CTGGAG CTGGAG(16/14) 67 CTGGAG BsiHKAI 76 GTGCTC G[AT]GC[AT]^C 298 GAGCTC Bsp1286I 76 GTGCTC G[AGT]GC[ACT]^C 298 GAGCTC BstNI 66 CCTGG CC^[AT]GG 234 CCAGG BstUI 271 CGCG CG^CG 330 CGCG BstYI 215 AGATCC [GA]^GATC[CT] 408 GGATCC DdeI 79 CTCAG C^T[ACGT]AG 91 CTGAG EcoRII 66 CCTGG ^CC[AT]GG 234 CCAGG HgaI 319 GACGC GACGC(5/10) 272 GCGTC HincII 145 GTCGAC GT[CT]^[GA]AC 422 GTCAAC HindII 145 GTCGAC GT[CT]^[GA]AC 422 GTCAAC HinfI 93 GAGTC G^A[ACGT]TC 384 GAATC HphI 378 GGTGA GGTGA(8/7) 224 TCACC MaeII 143 ACGT A^CGT 158 ACGT MflI 215 AGATCC [GA]^GATC[CT] 408 GGATCC MseI 418 TTAA T^TAA 479 TTAA MvaI 66 CCTGG CC^[AT]GG 234 CCAGG MvnI 271 CGCG CG^CG 330 CGCG Tru9I 418 TTAA T^TAA 479 TTAA XhoII 215 AGATCC [GA]^GATC[CT] 408 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### AciI AlwI BbvI BcnI BsaJI BsiYI BslI BssKI BstF5I Cac8I CfoI Cfr13I DpnII Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII ItaI MboI MnlI MspI MwoI NciI NdeII NlaIV Sau3AI Sau96I ScrFI TseI TspRI