Restriction map for pF1KB8372
##################################
# NO CUT ENZYMES (  134 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BalI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BfaI        BglI        BglII       BlnI        
BsaAI       BsaBI       BsaWI       BseAI       BseRI       
BsiEI       BsiWI       BsmBI       BsmFI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstXI       
BstZ17I     Bsu36I      ClaI        CpoI        DraI        
DraII       DrdI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoO109I    
EcoO65I     EcoRV       EcoT14I     EcoT22I     FbaI        
FseI        FspI        HindIII     HpaI        KpnI        
Ksp632I     MaeI        MamI        MfeI        MluI        
MluNI       MroI        MscI        MunI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SapI        SbfI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        Tth111I     Van91I      
XbaI        XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   67 enzymes) #
###################################

AccI          145  GTCGAC               GT^[AC][GT]AC
AcsI           13  GAATTC               [GA]^AATT[CT]
AcyI          343  GGCGCC               G[GA]^CG[CT]C
AfaI          120  GTAC                 GT^AC
AflII         417  CTTAAG               C^TTAAG
ApoI           13  GAATTC               [GA]^AATT[CT]
AseI          478  ATTAAT               AT^TAAT
AsnI          478  ATTAAT               AT^TAAT
AvaI          282  CCCGGG               C^[CT]CG[GA]G
AvaII         360  GGACC                G^G[AT]CC
BamHI         408  GGATCC               G^GATCC
BanI          343  GGCGCC               G^G[CT][GA]CC
BanII         298  GAGCTC               G[GA]GC[CT]^C
BbeI          343  GGCGCC               GGCGC^C
BbsI           33  GAAGAC               GAAGAC(2/6)
BfrI          417  CTTAAG               C^TTAAG
BlpI           78  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I       78  GCTCAGC              GC^T[ACGT]AGC
BpuAI          33  GAAGAC               GAAGAC(2/6)
BsaHI         343  GGCGCC               G[GA]^CG[CT]C
BsaI          313  GAGACC               GGTCTC(1/5)
BsgI          364  CTGCAC               GTGCAG(16/14)
BsmAI         313  GAGAC                GTCTC(1/5)
BsoBI         282  CCCGGG               C^[CT]CG[GA]G
BspMI         362  ACCTGC               ACCTGC(4/8)
BsrFI         287  GCCGGC               [GA]^CCGG[CT]
BsrI          472  CCAGT                ACTGG(1/-1)
BstAPI        366  GCACTGCGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII          78  GCTCAGC              GC^T[ACGT]AGC
Cfr10I        287  GCCGGC               [GA]^CCGG[CT]
DraIII        367  CACTGCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          329  CCGCGG               C^C[GA][CT]GG
EaeI          128  CGGCCA               [CT]^GGCC[GA]
EcoNI         228  CCTGATCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI          13  GAATTC               G^AATTC
FauI          328  CCCGC                CCCGC(4/6)
HaeII         343  GGCGCC               [GA]GCGC^[CT]
Hin1I         343  GGCGCC               G[GA]^CG[CT]C
KasI          343  GGCGCC               G^GCGCC
KspI          329  CCGCGG               CCGC^GG
MaeIII        379  GTGAC                ^GT[ACGT]AC
MboII          33  GAAGA                GAAGA(8/7)
MslI          395  CATCTCAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        329  CCGCGG               C[AC]G^C[GT]G
NaeI          287  GCCGGC               GCC^GGC
NarI          343  GGCGCC               GG^CGCC
NgoMIV        287  GCCGGC               G^CCGGC
NlaIII        456  CATG                 CATG^
PleI           93  GAGTC                GAGTC(4/5)
PshBI         478  ATTAAT               AT^TAAT
PstI          211  CTGCAG               CTGCA^G
RsaI          120  GTAC                 GT^AC
SacI          298  GAGCTC               GAGCT^C
SacII         329  CCGCGG               CCGC^GG
SalI          145  GTCGAC               G^TCGAC
SfaNI          61  GATGC                GCATC(5/9)
SfcI          211  CTGCAG               C^T[GA][CT]AG
SfoI          343  GGCGCC               GGC^GCC
SmaI          282  CCCGGG               CCC^GGG
SmlI          417  CTTAAG               C^T[CT][GA]AG
TaqI          146  TCGA                 T^CGA
TfiI          384  GAATC                G^A[AT]TC
Tsp45I        379  GTGAC                ^GT[GC]AC
Tsp509I        14  AATT                 ^AATT
TthHB8I       146  TCGA                 T^CGA
XmaCI         282  CCCGGG               C^CCGGG
XmaI          282  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccII         271  CGCG                 CG^CG
              330  CGCG                
AluI          134  AGCT                 AG^CT
              299  AGCT                
AspHI          76  GTGCTC               G[AT]GC[AT]^C
              298  GAGCTC              
BmyI           76  GTGCTC               G[AGT]GC[ACT]^C
              298  GAGCTC              
BpmI           23  CTGGAG               CTGGAG(16/14)
               67  CTGGAG              
BsiHKAI        76  GTGCTC               G[AT]GC[AT]^C
              298  GAGCTC              
Bsp1286I       76  GTGCTC               G[AGT]GC[ACT]^C
              298  GAGCTC              
BstNI          66  CCTGG                CC^[AT]GG
              234  CCAGG               
BstUI         271  CGCG                 CG^CG
              330  CGCG                
BstYI         215  AGATCC               [GA]^GATC[CT]
              408  GGATCC              
DdeI           79  CTCAG                C^T[ACGT]AG
               91  CTGAG               
EcoRII         66  CCTGG                ^CC[AT]GG
              234  CCAGG               
HgaI          319  GACGC                GACGC(5/10)
              272  GCGTC               
HincII        145  GTCGAC               GT[CT]^[GA]AC
              422  GTCAAC              
HindII        145  GTCGAC               GT[CT]^[GA]AC
              422  GTCAAC              
HinfI          93  GAGTC                G^A[ACGT]TC
              384  GAATC               
HphI          378  GGTGA                GGTGA(8/7)
              224  TCACC               
MaeII         143  ACGT                 A^CGT
              158  ACGT                
MflI          215  AGATCC               [GA]^GATC[CT]
              408  GGATCC              
MseI          418  TTAA                 T^TAA
              479  TTAA                
MvaI           66  CCTGG                CC^[AT]GG
              234  CCAGG               
MvnI          271  CGCG                 CG^CG
              330  CGCG                
Tru9I         418  TTAA                 T^TAA
              479  TTAA                
XhoII         215  AGATCC               [GA]^GATC[CT]
              408  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   33 enzymes) #
#########################################

AciI        AlwI        BbvI        BcnI        BsaJI       
BsiYI       BslI        BssKI       BstF5I      Cac8I       
CfoI        Cfr13I      DpnII       Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HpaII       
ItaI        MboI        MnlI        MspI        MwoI        
NciI        NdeII       NlaIV       Sau3AI      Sau96I      
ScrFI       TseI        TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com