Restriction map for
pF1KB8373
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# NO CUT ENZYMES ( 180 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BcnI BfrI BglI BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsgI BsiEI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspHI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I Cfr13I ClaI CpoI
DraI DraII DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FseI
FspI HaeII HaeIII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HphI KasI KpnI KspI
MaeII MamI MfeI MluI MluNI
MscI MslI MspA1I MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NlaIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI Sau96I SbfI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StuI StyI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccIII 195 TCCGGA T^CCGGA
AluI 68 AGCT AG^CT
AlwNI 23 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
BclI 59 TGATCA T^GATCA
BfaI 124 CTAG C^TAG
BglII 117 AGATCT A^GATCT
BsaJI 247 CCGTGG C^C[ACGT][ACGT]GG
BsaWI 195 TCCGGA [AT]^CCGG[AT]
BseAI 195 TCCGGA T^CCGGA
BseRI 508 GAGGAG GAGGAG(10/8)
BsmAI 462 GAGAC GTCTC(1/5)
BspEI 195 TCCGGA T^CCGGA
BsrDI 349 GCAATG GCAATG(2/0)
BstF5I 404 CATCC GGATG(2/0)
BstYI 117 AGATCT [GA]^GATC[CT]
DdeI 337 CTAAG C^T[ACGT]AG
DsaI 247 CCGTGG C^C[GA][CT]GG
FbaI 59 TGATCA T^GATCA
FokI 404 CATCC GGATG(9/13)
HapII 196 CCGG C^CGG
HpaII 196 CCGG C^CGG
MaeI 124 CTAG C^TAG
MaeIII 44 GTGAC ^GT[ACGT]AC
MflI 117 AGATCT [GA]^GATC[CT]
MroI 195 TCCGGA T^CCGGA
MseI 106 TTAA T^TAA
MspI 196 CCGG C^CGG
NspI 371 GCATGC [GA]CATG^[CT]
PflMI 156 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 192 GACTC GAGTC(4/5)
ScaI 485 AGTACT AGT^ACT
SmlI 271 CTTGAG C^T[CT][GA]AG
SphI 371 GCATGC GCATG^C
TaqI 476 TCGA T^CGA
TfiI 333 GAATC G^A[AT]TC
Tru9I 106 TTAA T^TAA
Tsp45I 44 GTGAC ^GT[GC]AC
TthHB8I 476 TCGA T^CGA
Van91I 156 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 117 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 7 AAATTC [GA]^AATT[CT]
323 AAATTC
AfaI 228 GTAC GT^AC
486 GTAC
AlwI 15 GATCC GGATC(4/5)
316 GGATC
ApoI 7 AAATTC [GA]^AATT[CT]
323 AAATTC
AspHI 142 GAGCAC G[AT]GC[AT]^C
184 GAGCAC
BmyI 142 GAGCAC G[AGT]GC[ACT]^C
184 GAGCAC
BsiHKAI 142 GAGCAC G[AT]GC[AT]^C
184 GAGCAC
BsiYI 156 CCACTGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
247 CCGTGGATTGG
BslI 156 CCACTGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
247 CCGTGGATTGG
Bsp1286I 142 GAGCAC G[AGT]GC[ACT]^C
184 GAGCAC
BsrI 188 ACTGG ACTGG(1/-1)
446 CCAGT
BssKI 162 CCTGG ^CC[ACGT]GG
342 CCAGG
BstNI 162 CCTGG CC^[AT]GG
342 CCAGG
Cac8I 69 GCTGGC GC[ACGT]^[ACGT]GC
371 GCATGC
EarI 425 GAAGAG CTCTTC(1/4)
451 GAAGAG
EcoRII 162 CCTGG ^CC[AT]GG
342 CCAGG
HinfI 192 GACTC G^A[ACGT]TC
333 GAATC
Ksp632I 425 GAAGAG CTCTTC(1/4)
451 GAAGAG
MvaI 162 CCTGG CC^[AT]GG
342 CCAGG
PstI 39 CTGCAG CTGCA^G
363 CTGCAG
RsaI 228 GTAC GT^AC
486 GTAC
ScrFI 162 CCTGG CC^[ACGT]GG
342 CCAGG
SfcI 39 CTGCAG C^T[GA][CT]AG
363 CTGCAG
Tsp509I 8 AATT ^AATT
324 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
AciI BbvI DpnII Fnu4HI ItaI
MboI MboII MnlI MwoI NdeII
NlaIII Sau3AI TseI TspRI