Restriction map for
pF1KB8376
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BamHI BbvCI
BciVI BclI BfrI BglII BlnI
BsaWI BseAI BsiWI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssHII
Bst1107I BstZ17I Bsu36I ClaI CpoI
DraI Eam1105I EarI Eco47III Eco81I
EcoRI EcoT22I FbaI FseI FspI
HindIII HpaI KpnI Ksp632I KspI
MfeI MluI MroI MseI MunI
NcoI NdeI NotI NruI NsiI
NspI PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tsp509I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AatII 703 GACGTC GACGT^C
AspI 898 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 1394 GCAGCGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1849 CTAG C^TAG
BglI 910 GCCTGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 1578 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1026 GGTCTC GGTCTC(1/5)
BsiEI 1139 CGGCCG CG[GA][CT]^CG
BsmI 924 GAATGC GAATGC(1/-1)
BspMI 591 GCAGGT ACCTGC(4/8)
BsrBI 1369 GAGCGG CCGCTC(-3/-3)
BsrDI 1346 GCAATG GCAATG(2/0)
BstAPI 606 GCACTTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 97 TTCGAA TT^CGAA
BstEII 882 GGTGACC G^GT[ACGT]ACC
BstPI 882 GGTGACC G^GT[ACGT]ACC
DraIII 607 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 826 GACGGGACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1139 CGGCCG C^GGCCG
EclXI 1139 CGGCCG C^GGCCG
Eco52I 1139 CGGCCG C^GGCCG
EcoNI 837 CCTCCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 882 GGTGACC G^GT[ACGT]ACC
EcoRV 406 GATATC GAT^ATC
EcoT14I 1820 CCAAGG C^C[AT][AT]GG
HincII 412 GTCAAC GT[CT]^[GA]AC
HindII 412 GTCAAC GT[CT]^[GA]AC
HinfI 1252 GAGTC G^A[ACGT]TC
MaeI 1849 CTAG C^TAG
MamI 1578 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI 1848 GCTAGC G^CTAGC
NspV 97 TTCGAA TT^CGAA
PflFI 898 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 636 CCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1252 GAGTC GAGTC(4/5)
SfuI 97 TTCGAA TT^CGAA
StuI 653 AGGCCT AGG^CCT
StyI 1820 CCAAGG C^C[AT][AT]GG
Tth111I 898 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 636 CCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AlwI 1092 GATCC GGATC(4/5)
1732 GGATC
AlwNI 1090 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
1285 CAGTGCCTG
BalI 933 TGGCCA TGG^CCA
1194 TGGCCA
BbrPI 105 CACGTG CAC^GTG
766 CACGTG
BbsI 895 GAAGAC GAAGAC(2/6)
217 GTCTTC
BlpI 309 GCTCAGC GC^T[ACGT]AGC
513 GCTGAGC
Bpu1102I 309 GCTCAGC GC^T[ACGT]AGC
513 GCTGAGC
BpuAI 895 GAAGAC GAAGAC(2/6)
217 GTCTTC
BsaAI 105 CACGTG [CT]AC^GT[GA]
766 CACGTG
BsmBI 111 CGTCTC CGTCTC(1/5)
1021 GAGACG
BsrFI 560 GCCGGC [GA]^CCGG[CT]
1137 GCCGGC
BstXI 422 CCAACATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1487 CCAAGAGCCTGG
BstYI 1091 AGATCC [GA]^GATC[CT]
1732 GGATCT
CelII 309 GCTCAGC GC^T[ACGT]AGC
513 GCTGAGC
Cfr10I 560 GCCGGC [GA]^CCGG[CT]
1137 GCCGGC
MflI 1091 AGATCC [GA]^GATC[CT]
1732 GGATCT
MluNI 933 TGGCCA TGG^CCA
1194 TGGCCA
MscI 933 TGGCCA TGG^CCA
1194 TGGCCA
NaeI 560 GCCGGC GCC^GGC
1137 GCCGGC
NgoMIV 560 GCCGGC G^CCGGC
1137 GCCGGC
PmaCI 105 CACGTG CAC^GTG
766 CACGTG
PmlI 105 CACGTG CAC^GTG
766 CACGTG
PpuMI 278 GGGACCT [GA]G^G[AT]CC[CT]
356 GGGACCT
PstI 676 CTGCAG CTGCA^G
1123 CTGCAG
PvuII 471 CAGCTG CAG^CTG
1126 CAGCTG
ScaI 341 AGTACT AGT^ACT
1166 AGTACT
SfcI 676 CTGCAG C^T[GA][CT]AG
1123 CTGCAG
XhoII 1091 AGATCC [GA]^GATC[CT]
1732 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaI AvaII BanI BanII
BbeI BbvI BcnI BmyI BpmI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BssSI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HpaII HphI ItaI
KasI MaeII MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI SfoI TaqI TseI
Tsp45I TspRI TthHB8I