Restriction map for
pF1KB8379
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# NO CUT ENZYMES ( 103 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AcyI AflII AgeI Aor51HI ApoI
AscI AseI AsnI Asp718I AviII
AvrII BalI BbeI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BsaBI BsaHI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssSI Bst1107I BstBI BstZ17I
ClaI CpoI DraI EarI Eco47III
EcoRI EcoRV EcoT22I FbaI FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MamI MfeI MluI MluNI
MroI MscI MseI MunI NarI
NdeI NheI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SspBI SspI SwaI TfiI
Tru9I XbaI XhoI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AhdI 1138 GACAACGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1786 GTGCAC G^TGCAC
AocI 1272 CCTGAGG CC^T[ACGT]AGG
ApaLI 1786 GTGCAC G^TGCAC
AspEI 1138 GACAACGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 221 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 952 CACGTG CAC^GTG
BbvCI 91 GCTGAGG CCTCAGC(-5/-2)
BglI 1327 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 952 CACGTG [CT]AC^GT[GA]
BsiEI 1639 CGGCCG CG[GA][CT]^CG
BsmI 149 GCATTC GAATGC(1/-1)
BsrBI 777 GAGCGG CCGCTC(-3/-3)
BsrDI 599 GCAATG GCAATG(2/0)
BsrFI 137 GCCGGC [GA]^CCGG[CT]
BssHII 1665 GCGCGC G^CGCGC
BstEII 667 GGTGACC G^GT[ACGT]ACC
BstPI 667 GGTGACC G^GT[ACGT]ACC
Bsu36I 1272 CCTGAGG CC^T[ACGT]AGG
Cfr10I 137 GCCGGC [GA]^CCGG[CT]
DraIII 850 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1303 GACCACAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1639 CGGCCG C^GGCCG
Eam1105I 1138 GACAACGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1639 CGGCCG C^GGCCG
Eco52I 1639 CGGCCG C^GGCCG
Eco81I 1272 CCTGAGG CC^T[ACGT]AGG
EcoO65I 667 GGTGACC G^GT[ACGT]ACC
HinfI 1144 GAGTC G^A[ACGT]TC
MslI 339 CACCGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 137 GCCGGC GCC^GGC
NcoI 215 CCATGG C^CATGG
NgoMIV 137 GCCGGC G^CCGGC
NotI 1638 GCGGCCGC GC^GGCCGC
NspI 722 GCATGC [GA]CATG^[CT]
PflFI 221 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1144 GAGTC GAGTC(4/5)
PmaCI 952 CACGTG CAC^GTG
PmlI 952 CACGTG CAC^GTG
SacI 1773 GAGCTC GAGCT^C
SbfI 1062 CCTGCAGG CCTGCA^GG
SmlI 962 CTCAAG C^T[CT][GA]AG
SphI 722 GCATGC GCATG^C
Sse8387I 1062 CCTGCAGG CCTGCA^GG
StuI 1673 AGGCCT AGG^CCT
Tsp509I 1706 AATT ^AATT
Tth111I 221 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 803 CCACGGTGGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AccI 271 GTAGAC GT^[AC][GT]AC
1366 GTCTAC
AflIII 392 ACGTGT A^C[GA][CT]GT
485 ACGTGT
ApaI 171 GGGCCC GGGCC^C
1118 GGGCCC
AspHI 1773 GAGCTC G[AT]GC[AT]^C
1786 GTGCAC
BamHI 1460 GGATCC G^GATCC
1554 GGATCC
BbsI 159 GAAGAC GAAGAC(2/6)
973 GTCTTC
BpuAI 159 GAAGAC GAAGAC(2/6)
973 GTCTTC
BsaI 1571 GAGACC GGTCTC(1/5)
607 GGTCTC
BsaWI 240 ACCGGA [AT]^CCGG[AT]
977 TCCGGT
BsgI 22 CTGCAC GTGCAG(16/14)
640 GTGCAG
BsiHKAI 1773 GAGCTC G[AT]GC[AT]^C
1786 GTGCAC
BsmFI 219 GGGAC GGGAC(10/14)
735 GTCCC
BstXI 836 CCACGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1329 CCAGCTGGCTGG
EcoNI 256 CCTTGGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1272 CCTGAGGGAGG
HaeII 1714 AGCGCC [GA]GCGC^[CT]
1742 AGCGCC
PflMI 303 CCAATATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
849 CCACCTTGTGG
PvuII 841 CAGCTG CAG^CTG
1330 CAGCTG
Van91I 303 CCAATATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
849 CCACCTTGTGG
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# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI Asp700I AvaI AvaII BanI
BanII BbvI BcnI BlpI BmyI
BpmI Bpu1102I BsaJI BseRI BsiYI
BslI BsmAI BsoBI Bsp1286I BspMI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI StyI
TaqI TseI Tsp45I TspRI TthHB8I
XhoII XmaCI XmaI XmnI