Restriction map for
pF1KB8381
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# NO CUT ENZYMES ( 129 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Aor51HI ApaI AscI
Asp718I AspEI AspI AviII BalI
BamHI BbeI BbrPI BbsI BbvCI
BciVI BclI BfrI BglI BglII
BpuAI BsaHI BsaI BsaWI BseAI
BseRI BsiEI BsiWI BsmBI Bsp1407I
BspEI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstEII
BstPI BstUI BstYI BstZ17I CfoI
Cfr10I CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I FauI FbaI FseI FspI
HaeII HhaI Hin1I HinP1I HindIII
KasI KpnI KspI MfeI MflI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NdeI NgoMIV
NotI NruI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I XcmI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Alw44I 565 GTGCAC G^TGCAC
AlwNI 91 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 988 CCTCAGG CC^T[ACGT]AGG
ApaLI 565 GTGCAC G^TGCAC
AseI 331 ATTAAT AT^TAAT
AsnI 331 ATTAAT AT^TAAT
Asp700I 917 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 841 CCCGAG C^[CT]CG[GA]G
AvaII 531 GGACC G^G[AT]CC
AvrII 487 CCTAGG C^CTAGG
BanII 8 GGGCTC G[GA]GC[CT]^C
BcgI 1011 GCATCGAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 487 CCTAGG C^CTAGG
BsaAI 849 CACGTA [CT]AC^GT[GA]
BsmFI 530 GGGAC GGGAC(10/14)
BsmI 293 GAATGC GAATGC(1/-1)
BsoBI 841 CCCGAG C^[CT]CG[GA]G
BspDI 481 ATCGAT AT^CGAT
BspHI 777 TCATGA T^CATGA
BsrDI 134 CATTGC GCAATG(2/0)
BstAPI 1002 GCAGTGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 290 TTCGAA TT^CGAA
BstXI 1098 CCAAATCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 988 CCTCAGG CC^T[ACGT]AGG
ClaI 481 ATCGAT AT^CGAT
DraII 530 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 822 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 835 CCATGG C^C[GA][CT]GG
Eco81I 988 CCTCAGG CC^T[ACGT]AGG
EcoO109I 530 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 457 GATATC GAT^ATC
EcoT22I 1136 ATGCAT ATGCA^T
HgaI 1158 GCGTC GACGC(5/10)
HincII 1167 GTTAAC GT[CT]^[GA]AC
HindII 1167 GTTAAC GT[CT]^[GA]AC
HpaI 1167 GTTAAC GTT^AAC
MaeIII 958 GTCAC ^GT[ACGT]AC
MslI 1243 CATGAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 835 CCATGG C^CATGG
NheI 70 GCTAGC G^CTAGC
NsiI 1136 ATGCAT ATGCA^T
NspI 1444 GCATGT [GA]CATG^[CT]
NspV 290 TTCGAA TT^CGAA
PpuMI 530 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 331 ATTAAT AT^TAAT
RcaI 777 TCATGA T^CATGA
SfuI 290 TTCGAA TT^CGAA
Tsp45I 958 GTCAC ^GT[GC]AC
XbaI 1293 TCTAGA T^CTAGA
XmnI 917 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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BanI 106 GGTGCC G^G[CT][GA]CC
618 GGTGCC
BcnI 47 CCGGG CC^[GC]GG
80 CCGGG
BlpI 997 GCTCAGC GC^T[ACGT]AGC
1333 GCTCAGC
Bpu1102I 997 GCTCAGC GC^T[ACGT]AGC
1333 GCTCAGC
BsaBI 1095 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1483 GATAGCTATC
BsgI 55 GTGCAG GTGCAG(16/14)
725 GTGCAG
BsrI 96 ACTGG ACTGG(1/-1)
955 CCAGT
BstF5I 1151 GGATG GGATG(2/0)
1248 GGATG
Cac8I 70 GCTAGC GC[ACGT]^[ACGT]GC
691 GCCAGC
CelII 997 GCTCAGC GC^T[ACGT]AGC
1333 GCTCAGC
EarI 154 GAAGAG CTCTTC(1/4)
348 GAAGAG
EcoRI 443 GAATTC G^AATTC
1394 GAATTC
FokI 1151 GGATG GGATG(9/13)
1248 GGATG
Ksp632I 154 GAAGAG CTCTTC(1/4)
348 GAAGAG
MaeII 259 ACGT A^CGT
850 ACGT
MamI 1095 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1483 GATAGCTATC
MspA1I 693 CAGCTG C[AC]G^C[GT]G
1307 CAGCTG
NciI 47 CCGGG CC^[GC]GG
80 CCGGG
PflMI 90 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
868 CCATCACCTGG
PleI 1255 GACTC GAGTC(4/5)
157 GAGTC
PstI 1112 CTGCAG CTGCA^G
1195 CTGCAG
PvuII 693 CAGCTG CAG^CTG
1307 CAGCTG
Van91I 90 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
868 CCATCACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AspHI BbvI BfaI BmyI BpmI
BsaJI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BssKI BstNI Cfr13I DdeI
DpnII EaeI EcoRII EcoT14I Fnu4HI
HaeIII HapII HinfI HpaII HphI
ItaI MaeI MboI MboII MnlI
MseI MspI MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I