Restriction map for
pF1KB8384
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII AccII AccIII AclI AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BalI BcgI BclI
BlnI BlpI Bpu1102I BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BspDI BspEI BspHI BssHII BssSI
Bst1107I BstBI BstF5I BstUI BstXI
BstZ17I CelII ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoRV EcoT22I FbaI
FokI FseI FspI HgaI HincII
HindII HindIII HpaI KspI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI StuI SwaI Tth111I
XbaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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Acc65I 106 GGTACC G^GTACC
AccI 1753 GTAGAC GT^[AC][GT]AC
AcyI 1400 GGCGCC G[GA]^CG[CT]C
AflII 736 CTTAAG C^TTAAG
AflIII 266 ACATGT A^C[GA][CT]GT
Asp718I 106 GGTACC G^GTACC
BbeI 1400 GGCGCC GGCGC^C
BbrPI 902 CACGTG CAC^GTG
BbsI 498 GAAGAC GAAGAC(2/6)
BbvCI 64 GCTGAGG CCTCAGC(-5/-2)
BciVI 1368 GGATAC GTATCC(6/5)
BfrI 736 CTTAAG C^TTAAG
BglI 970 GCCCTAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 422 AGATCT A^GATCT
BpuAI 498 GAAGAC GAAGAC(2/6)
BsaAI 902 CACGTG [CT]AC^GT[GA]
BsaBI 343 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1400 GGCGCC G[GA]^CG[CT]C
BseRI 259 GAGGAG GAGGAG(10/8)
Bsp1407I 156 TGTACA T^GTACA
BspLU11I 266 ACATGT A^CATGT
BsrDI 254 GCAATG GCAATG(2/0)
BsrFI 889 GCCGGT [GA]^CCGG[CT]
BsrGI 156 TGTACA T^GTACA
CfoI 1401 GCGC GCG^C
Cfr10I 889 GCCGGT [GA]^CCGG[CT]
EaeI 456 TGGCCG [CT]^GGCC[GA]
EcoRI 1437 GAATTC G^AATTC
FauI 680 CCCGC CCCGC(4/6)
HaeII 1400 GGCGCC [GA]GCGC^[CT]
HhaI 1401 GCGC GCG^C
Hin1I 1400 GGCGCC G[GA]^CG[CT]C
HinP1I 1401 GCGC G^CGC
HphI 1261 GGTGA GGTGA(8/7)
KasI 1400 GGCGCC G^GCGCC
KpnI 106 GGTACC GGTAC^C
MaeII 903 ACGT A^CGT
MamI 343 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 1400 GGCGCC GG^CGCC
PaeR7I 587 CTCGAG C^TCGAG
PmaCI 902 CACGTG CAC^GTG
PmlI 902 CACGTG CAC^GTG
PpuMI 210 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 313 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 958 CTGCAG CTGCA^G
SapI 301 GAAGAGC GCTCTTC(1/4)
SbfI 957 CCTGCAGG CCTGCA^GG
SfoI 1400 GGCGCC GGC^GCC
Sse8387I 957 CCTGCAGG CCTGCA^GG
SspBI 156 TGTACA T^GTACA
SspI 473 AATATT AAT^ATT
TfiI 1471 GAATC G^A[AT]TC
XcmI 1495 CCATGGGAGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 587 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwNI 1124 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1457 CAGCAGCTG
AocI 206 CCTCAGG CC^T[ACGT]AGG
862 CCTAAGG
BfaI 973 CTAG C^TAG
1103 CTAG
BpmI 221 CTGGAG CTGGAG(16/14)
1535 CTGGAG
BsmFI 1212 GGGAC GGGAC(10/14)
1681 GTCCC
BspMI 956 ACCTGC ACCTGC(4/8)
960 GCAGGT
BsrBI 637 GAGCGG CCGCTC(-3/-3)
910 GAGCGG
BstEII 1261 GGTGACC G^GT[ACGT]ACC
1490 GGTTACC
BstPI 1261 GGTGACC G^GT[ACGT]ACC
1490 GGTTACC
Bsu36I 206 CCTCAGG CC^T[ACGT]AGG
862 CCTAAGG
DrdI 501 GACCTTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1279 GACAAGGGTGTC
Eco81I 206 CCTCAGG CC^T[ACGT]AGG
862 CCTAAGG
EcoO65I 1261 GGTGACC G^GT[ACGT]ACC
1490 GGTTACC
MaeI 973 CTAG C^TAG
1103 CTAG
MslI 724 CATGCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1140 CATTAACATG
PflMI 796 CCAAAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
817 CCAGAGGGTGG
SacI 933 GAGCTC GAGCT^C
1199 GAGCTC
ScaI 356 AGTACT AGT^ACT
1584 AGTACT
SmaI 1001 CCCGGG CCC^GGG
1226 CCCGGG
Tsp45I 626 GTGAC ^GT[GC]AC
1262 GTGAC
Van91I 796 CCAAAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
817 CCAGAGGGTGG
XmaCI 1001 CCCGGG C^CCGGG
1226 CCCGGG
XmaI 1001 CCCGGG C^CCGGG
1226 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApaI ApoI AspHI AvaI AvaII
BamHI BanI BanII BbvI BcnI
BmyI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsoBI Bsp1286I BsrI
BssKI BstAPI BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII DsaI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
HaeIII HapII HinfI HpaII ItaI
Ksp632I MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI Tru9I TseI
Tsp509I TspRI TthHB8I XhoII