Restriction map for
pF1KB8387
##################################
# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII AccIII AclI AcyI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI AspEI
AspHI AviII BalI BamHI BanII
BbeI BbrPI BbsI BbvCI BcgI
BclI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BseAI BseRI BsiHKAI
BsmBI Bsp1407I BspDI BspEI BspMI
BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstEII BstPI Bsu36I CelII
ClaI CpoI DraII DraIII EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HgaI Hin1I HincII
HindII HpaI KasI Ksp632I MamI
MfeI MluI MluNI MroI MscI
MunI NarI NdeI NheI NotI
NruI NsiI PacI PaeR7I PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccI 499 GTATAC GT^[AC][GT]AC
AccII 24 CGCG CG^CG
AflIII 1683 ACATGT A^C[GA][CT]GT
AgeI 781 ACCGGT A^CCGGT
AspI 464 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1112 CTCGGG C^[CT]CG[GA]G
AvrII 1272 CCTAGG C^CTAGG
BciVI 1188 GGATAC GTATCC(6/5)
BcnI 7 CCCGG CC^[GC]GG
BglI 19 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1443 AGATCT A^GATCT
BlnI 1272 CCTAGG C^CTAGG
BmyI 1290 GGGCAC G[AGT]GC[ACT]^C
BsaWI 781 ACCGGT [AT]^CCGG[AT]
BsgI 962 GTGCAG GTGCAG(16/14)
BsiEI 99 CGACCG CG[GA][CT]^CG
BsiWI 896 CGTACG C^GTACG
BsoBI 1112 CTCGGG C^[CT]CG[GA]G
Bsp1286I 1290 GGGCAC G[AGT]GC[ACT]^C
BspLU11I 1683 ACATGT A^CATGT
Bst1107I 499 GTATAC GTA^TAC
BstBI 449 TTCGAA TT^CGAA
BstUI 24 CGCG CG^CG
BstZ17I 499 GTATAC GTA^TAC
Cac8I 56 GCCGGC GC[ACGT]^[ACGT]GC
DraI 757 TTTAAA TTT^AAA
DrdI 461 GACGACGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 14 TGGCCG [CT]^GGCC[GA]
HindIII 135 AAGCTT A^AGCTT
HphI 717 GGTGA GGTGA(8/7)
KspI 23 CCGCGG CCGC^GG
MaeII 146 ACGT A^CGT
MslI 1224 CACTTGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 23 CCGCGG C[AC]G^C[GT]G
MvnI 24 CGCG CG^CG
NaeI 56 GCCGGC GCC^GGC
NciI 7 CCCGG CC^[GC]GG
NcoI 981 CCATGG C^CATGG
NgoMIV 56 GCCGGC G^CCGGC
NspI 1683 ACATGT [GA]CATG^[CT]
NspV 449 TTCGAA TT^CGAA
PflFI 464 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 166 CCATATATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 781 ACCGGT A^CCGGT
PstI 1196 CTGCAG CTGCA^G
SacII 23 CCGCGG CCGC^GG
SfuI 449 TTCGAA TT^CGAA
TfiI 1018 GAATC G^A[AT]TC
Tsp45I 124 GTGAC ^GT[GC]AC
Tth111I 464 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 166 CCATATATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 438 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 175 GGTACC G^GTACC
977 GGTACC
AflII 307 CTTAAG C^TTAAG
346 CTTAAG
Asp718I 175 GGTACC G^GTACC
977 GGTACC
BbvI 1219 GCAGC GCAGC(8/12)
1195 GCTGC
BfrI 307 CTTAAG C^TTAAG
346 CTTAAG
BsaI 1582 GAGACC GGTCTC(1/5)
1308 GGTCTC
BsmAI 1582 GAGAC GTCTC(1/5)
1309 GTCTC
BsmFI 702 GGGAC GGGAC(10/14)
470 GTCCC
BsmI 1107 GAATGC GAATGC(1/-1)
1315 GCATTC
BspHI 953 TCATGA T^CATGA
1425 TCATGA
BsrFI 56 GCCGGC [GA]^CCGG[CT]
781 ACCGGT
BsrI 394 ACTGG ACTGG(1/-1)
431 CCAGT
BstXI 1223 CCACTTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1439 CCACAGATCTGG
BstYI 882 AGATCC [GA]^GATC[CT]
1443 AGATCT
CfoI 287 GCGC GCG^C
915 GCGC
Cfr10I 56 GCCGGC [GA]^CCGG[CT]
781 ACCGGT
DsaI 23 CCGCGG C^C[GA][CT]GG
981 CCATGG
EcoT14I 981 CCATGG C^C[AT][AT]GG
1272 CCTAGG
HhaI 287 GCGC GCG^C
915 GCGC
HinP1I 287 GCGC G^CGC
915 GCGC
KpnI 175 GGTACC GGTAC^C
977 GGTACC
MflI 882 AGATCC [GA]^GATC[CT]
1443 AGATCT
RcaI 953 TCATGA T^CATGA
1425 TCATGA
SfcI 156 CTGTAG C^T[GA][CT]AG
1196 CTGCAG
SspI 630 AATATT AAT^ATT
1609 AATATT
StyI 981 CCATGG C^C[AT][AT]GG
1272 CCTAGG
TaqI 450 TCGA T^CGA
1680 TCGA
TseI 1195 GCTGC G^C[AT]GC
1219 GCAGC
TthHB8I 450 TCGA T^CGA
1680 TCGA
XhoII 882 AGATCC [GA]^GATC[CT]
1443 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AvaII BanI BfaI
BpmI BsaJI BsiYI BslI BssKI
BstF5I BstNI Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII ItaI MaeI MaeIII
MboI MboII MnlI MseI MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI Tru9I Tsp509I TspRI
XmnI