Restriction map for
pF1KB8388
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# NO CUT ENZYMES ( 110 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI AocI
Aor51HI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BciVI BclI BfaI
BfrI BglII BlnI BsaBI BsaWI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII Bst1107I BstAPI BstBI BstYI
BstZ17I Bsu36I ClaI CpoI DraI
DrdI Eam1105I Eco47III Eco81I EcoNI
EcoRI EcoRV EcoT22I FbaI FspI
HindIII HpaI KpnI MaeI MamI
MfeI MflI MluI MroI MunI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmeI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI SbfI
ScaI SexAI SfaNI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tth111I
XbaI XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 762 GACGTC GACGT^C
AlwI 653 GGATC GGATC(4/5)
BalI 137 TGGCCA TGG^CCA
BcgI 1391 GCAAGCACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaI 721 GAGACC GGTCTC(1/5)
BsgI 1192 GTGCAG GTGCAG(16/14)
BsmBI 10 GAGACG CGTCTC(1/5)
BspMI 423 GCAGGT ACCTGC(4/8)
BssSI 901 CACGAG CACGAG(-5/-1)
BstXI 128 CCAATAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII 654 GATC ^GATC
DraIII 1159 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT14I 1700 CCATGG C^C[AT][AT]GG
FseI 1596 GGCCGGCC GGCCGG^CC
HgaI 12 GACGC GACGC(5/10)
HincII 1585 GTCAAC GT[CT]^[GA]AC
HindII 1585 GTCAAC GT[CT]^[GA]AC
KspI 323 CCGCGG CCGC^GG
MboI 654 GATC ^GATC
MluNI 137 TGGCCA TGG^CCA
MscI 137 TGGCCA TGG^CCA
MseI 392 TTAA T^TAA
MslI 1470 CATCACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 1700 CCATGG C^CATGG
NdeII 654 GATC ^GATC
PpuMI 589 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 1698 GACCATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1375 CTGCAG CTGCA^G
SacI 892 GAGCTC GAGCT^C
SacII 323 CCGCGG CCGC^GG
Sau3AI 654 GATC ^GATC
SfcI 1375 CTGCAG C^T[GA][CT]AG
SmaI 646 CCCGGG CCC^GGG
StuI 977 AGGCCT AGG^CCT
StyI 1700 CCATGG C^C[AT][AT]GG
TaqI 457 TCGA T^CGA
Tru9I 392 TTAA T^TAA
Tsp509I 680 AATT ^AATT
TthHB8I 457 TCGA T^CGA
XmaCI 646 CCCGGG C^CCGGG
XmaI 646 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 324 CGCG CG^CG
940 CGCG
Alw44I 1477 GTGCAC G^TGCAC
1654 GTGCAC
ApaLI 1477 GTGCAC G^TGCAC
1654 GTGCAC
AvaI 646 CCCGGG C^[CT]CG[GA]G
964 CCCGAG
AvaII 590 GGACC G^G[AT]CC
1035 GGACC
BbrPI 68 CACGTG CAC^GTG
857 CACGTG
BbsI 759 GAAGAC GAAGAC(2/6)
1215 GAAGAC
BbvCI 292 CCTCAGC CCTCAGC(-5/-2)
550 CCTCAGC
BlpI 906 GCTGAGC GC^T[ACGT]AGC
1062 GCTGAGC
Bpu1102I 906 GCTGAGC GC^T[ACGT]AGC
1062 GCTGAGC
BpuAI 759 GAAGAC GAAGAC(2/6)
1215 GAAGAC
BsaAI 68 CACGTG [CT]AC^GT[GA]
857 CACGTG
BsiEI 1595 CGGCCG CG[GA][CT]^CG
1634 CGGCCG
BsoBI 646 CCCGGG C^[CT]CG[GA]G
964 CCCGAG
BsrBI 82 GAGCGG CCGCTC(-3/-3)
847 GAGCGG
BstEII 279 GGTGACC G^GT[ACGT]ACC
1704 GGTCACC
BstPI 279 GGTGACC G^GT[ACGT]ACC
1704 GGTCACC
BstUI 324 CGCG CG^CG
940 CGCG
CelII 906 GCTGAGC GC^T[ACGT]AGC
1062 GCTGAGC
EagI 1595 CGGCCG C^GGCCG
1634 CGGCCG
EarI 639 GAAGAG CTCTTC(1/4)
813 GAAGAG
EclXI 1595 CGGCCG C^GGCCG
1634 CGGCCG
Eco52I 1595 CGGCCG C^GGCCG
1634 CGGCCG
EcoO65I 279 GGTGACC G^GT[ACGT]ACC
1704 GGTCACC
Ksp632I 639 GAAGAG CTCTTC(1/4)
813 GAAGAG
MvnI 324 CGCG CG^CG
940 CGCG
PmaCI 68 CACGTG CAC^GTG
857 CACGTG
PmlI 68 CACGTG CAC^GTG
857 CACGTG
SmlI 895 CTCAAG C^T[CT][GA]AG
1735 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwNI
ApaI AspHI BanI BanII BbeI
BbvI BcnI BglI BmyI BpmI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr10I Cfr13I DdeI DraII
DsaI EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI MaeII MaeIII
MboII MnlI MspA1I MspI MvaI
MwoI NaeI NarI NciI NgoMIV
NlaIII NlaIV PflMI PleI PvuII
RsaI Sau96I ScrFI SfoI TseI
Tsp45I TspRI Van91I