Restriction map for
pF1KB7636
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AviII BanI
BbeI BbrPI BbvCI BcgI BciVI
BlpI Bpu1102I BsaHI BsaI BsiEI
BsiWI BsmAI BsmBI Bsp1407I BspDI
BspLU11I BspMI BsrBI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstPI
BstUI Bsu36I CelII CfoI ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FseI FspI
HaeII HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MfeI MluI MunI
MvnI NarI NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 751 GTATAC GT^[AC][GT]AC
AccIII 270 TCCGGA T^CCGGA
AflII 862 CTTAAG C^TTAAG
AflIII 638 ACGTGT A^C[GA][CT]GT
AspHI 822 GTGCTC G[AT]GC[AT]^C
AspI 588 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 64 CCCGAG C^[CT]CG[GA]G
BamHI 813 GGATCC G^GATCC
BanII 1072 GAGCCC G[GA]GC[CT]^C
BbsI 556 GAAGAC GAAGAC(2/6)
BclI 264 TGATCA T^GATCA
BfrI 862 CTTAAG C^TTAAG
BglII 725 AGATCT A^GATCT
BpuAI 556 GAAGAC GAAGAC(2/6)
BsaAI 520 TACGTG [CT]AC^GT[GA]
BsaWI 270 TCCGGA [AT]^CCGG[AT]
BseAI 270 TCCGGA T^CCGGA
BsiHKAI 822 GTGCTC G[AT]GC[AT]^C
BsoBI 64 CCCGAG C^[CT]CG[GA]G
BspEI 270 TCCGGA T^CCGGA
BsrDI 1067 GCAATG GCAATG(2/0)
Bst1107I 751 GTATAC GTA^TAC
BstZ17I 751 GTATAC GTA^TAC
Cac8I 1055 GCCGGC GC[ACGT]^[ACGT]GC
DdeI 986 CTGAG C^T[ACGT]AG
EcoNI 654 CCTAGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 264 TGATCA T^GATCA
HgaI 559 GACGC GACGC(5/10)
MboII 556 GAAGA GAAGA(8/7)
MroI 270 TCCGGA T^CCGGA
NaeI 1055 GCCGGC GCC^GGC
NgoMIV 1055 GCCGGC G^CCGGC
NspI 1122 GCATGT [GA]CATG^[CT]
PflFI 588 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 954 GACCGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 226 CTGCAG CTGCA^G
SmlI 862 CTTAAG C^T[CT][GA]AG
TaqI 936 TCGA T^CGA
Tth111I 588 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 936 TCGA T^CGA
XcmI 188 CCATGGAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AciI 1065 CCGC CCGC(-3/-1)
22 GCGG
AcsI 502 AAATTT [GA]^AATT[CT]
604 AAATTC
AlwNI 34 CAGTCGCTG CAG[ACGT][ACGT][ACGT]^CTG
308 CAGCCACTG
ApoI 502 AAATTT [GA]^AATT[CT]
604 AAATTC
AvaII 42 GGTCC G^G[AT]CC
1021 GGTCC
AvrII 654 CCTAGG C^CTAGG
966 CCTAGG
BalI 92 TGGCCA TGG^CCA
200 TGGCCA
BcnI 45 CCCGG CC^[GC]GG
956 CCGGG
BglI 248 GCCAAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
925 GCCAACACGGC
BlnI 654 CCTAGG C^CTAGG
966 CCTAGG
BsgI 535 CTGCAC GTGCAG(16/14)
598 CTGCAC
BsmI 123 GCATTC GAATGC(1/-1)
562 GCATTC
BspHI 584 TCATGA T^CATGA
999 TCATGA
BsrFI 18 ACCGGC [GA]^CCGG[CT]
1055 GCCGGC
BstXI 540 CCATGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
770 CCAAAGCAGTGG
Cfr10I 18 ACCGGC [GA]^CCGG[CT]
1055 GCCGGC
DsaI 188 CCATGG C^C[GA][CT]GG
540 CCATGG
HphI 634 GGTGA GGTGA(8/7)
572 TCACC
MaeII 521 ACGT A^CGT
638 ACGT
MaeIII 83 GTGAC ^GT[ACGT]AC
635 GTGAC
MluNI 92 TGGCCA TGG^CCA
200 TGGCCA
MscI 92 TGGCCA TGG^CCA
200 TGGCCA
MspA1I 362 CAGCTG C[AC]G^C[GT]G
787 CAGCTG
MwoI 248 GCCAAGAGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
925 GCCAACACGGC
NciI 45 CCCGG CC^[GC]GG
956 CCGGG
NcoI 188 CCATGG C^CATGG
540 CCATGG
NdeI 140 CATATG CA^TATG
1016 CATATG
PvuII 362 CAGCTG CAG^CTG
787 CAGCTG
RcaI 584 TCATGA T^CATGA
999 TCATGA
SspI 483 AATATT AAT^ATT
716 AATATT
TfiI 1082 GAATC G^A[AT]TC
1113 GAATC
Tsp45I 83 GTGAC ^GT[GC]AC
635 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AfaI AluI AlwI BbvI BfaI
BmyI BpmI BsaBI BsaJI BseRI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cfr13I
DpnII EaeI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
ItaI MaeI MamI MboI MflI
MnlI MseI MslI MspI MvaI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI Tru9I TseI Tsp509I TspRI
XhoII