Restriction map for
pF1KB8399
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvrII BalI
BamHI BbrPI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsiWI BsmI Bsp1407I BspDI BspHI
BspLU11I BspMI BsrGI BsrI BssHII
Bst1107I BstAPI BstBI BstZ17I Bsu36I
CelII ClaI CpoI DdeI DraI
DraIII DrdI Eam1105I EarI Eco47III
Eco81I EcoNI EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HpaI KpnI Ksp632I KspI MaeI
MaeII MamI MboII MfeI MluI
MluNI MscI MseI MspA1I MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI TfiI
Tru9I Tsp509I TspRI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccII 540 CGCG CG^CG
AccIII 663 TCCGGA T^CCGGA
AfaI 348 GTAC GT^AC
ApaI 36 GGGCCC GGGCC^C
AspHI 15 GAGCAC G[AT]GC[AT]^C
AvaII 177 GGACC G^G[AT]CC
AviII 137 TGCGCA TGC^GCA
BglI 364 GCCTCGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 655 AGATCT A^GATCT
BpmI 562 CTGGAG CTGGAG(16/14)
BsaI 51 GGTCTC GGTCTC(1/5)
BseAI 663 TCCGGA T^CCGGA
BseRI 565 GAGGAG GAGGAG(10/8)
BsgI 388 CTGCAC GTGCAG(16/14)
BsiEI 188 CGGCCG CG[GA][CT]^CG
BsiHKAI 15 GAGCAC G[AT]GC[AT]^C
BsmFI 581 GGGAC GGGAC(10/14)
BspEI 663 TCCGGA T^CCGGA
BsrDI 299 GCAATG GCAATG(2/0)
BsrFI 235 ACCGGC [GA]^CCGG[CT]
BssSI 636 CTCGTG CACGAG(-5/-1)
BstUI 540 CGCG CG^CG
BstYI 655 AGATCT [GA]^GATC[CT]
Cfr10I 235 ACCGGC [GA]^CCGG[CT]
EaeI 188 CGGCCG [CT]^GGCC[GA]
EagI 188 CGGCCG C^GGCCG
EclXI 188 CGGCCG C^GGCCG
Eco52I 188 CGGCCG C^GGCCG
FspI 137 TGCGCA TGC^GCA
MflI 655 AGATCT [GA]^GATC[CT]
MroI 663 TCCGGA T^CCGGA
MvnI 540 CGCG CG^CG
PstI 574 CTGCAG CTGCA^G
RsaI 348 GTAC GT^AC
SfcI 574 CTGCAG C^T[GA][CT]AG
SgrAI 234 CACCGGCG C[GA]^CCGG[CT]G
Tsp45I 326 GTCAC ^GT[GC]AC
XhoII 655 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 90 GGCGCC G[GA]^CG[CT]C
252 GGCGCC
BanII 36 GGGCCC G[GA]GC[CT]^C
354 GGGCTC
BbeI 90 GGCGCC GGCGC^C
252 GGCGCC
BsaHI 90 GGCGCC G[GA]^CG[CT]C
252 GGCGCC
BsaWI 110 ACCGGA [AT]^CCGG[AT]
663 TCCGGA
BsmBI 313 GAGACG CGTCTC(1/5)
335 GAGACG
BsrBI 27 CCGCTC CCGCTC(-3/-3)
144 CCGCTC
BstEII 304 GGTTACC G^GT[ACGT]ACC
325 GGTCACC
BstF5I 341 GGATG GGATG(2/0)
375 GGATG
BstPI 304 GGTTACC G^GT[ACGT]ACC
325 GGTCACC
BstXI 294 CCATGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
446 CCAAGTCTGTGG
DpnII 555 GATC ^GATC
656 GATC
DraII 35 GGGGCCC [GA]G^G[ACGT]CC[CT]
36 GGGCCCC
DsaI 128 CCATGG C^C[GA][CT]GG
294 CCATGG
EcoO109I 35 GGGGCCC [GA]G^G[ACGT]CC[CT]
36 GGGCCCC
EcoO65I 304 GGTTACC G^GT[ACGT]ACC
325 GGTCACC
EcoT14I 128 CCATGG C^C[AT][AT]GG
294 CCATGG
FokI 341 GGATG GGATG(9/13)
375 GGATG
HgaI 538 GACGC GACGC(5/10)
274 GCGTC
Hin1I 90 GGCGCC G[GA]^CG[CT]C
252 GGCGCC
HphI 544 GGTGA GGTGA(8/7)
327 TCACC
KasI 90 GGCGCC G^GCGCC
252 GGCGCC
MaeIII 305 GTTAC ^GT[ACGT]AC
326 GTCAC
MboI 555 GATC ^GATC
656 GATC
NarI 90 GGCGCC GG^CGCC
252 GGCGCC
NcoI 128 CCATGG C^CATGG
294 CCATGG
NdeII 555 GATC ^GATC
656 GATC
Sau3AI 555 GATC ^GATC
656 GATC
SfoI 90 GGCGCC GGC^GCC
252 GGCGCC
StyI 128 CCATGG C^C[AT][AT]GG
294 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AluI AvaI BanI BbvI
BcnI BmyI BsaJI BsiYI BslI
BsmAI BsoBI Bsp1286I BssKI BstNI
Cac8I CfoI Cfr13I EcoRII FauI
Fnu4HI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MnlI
MslI MspI MvaI MwoI NciI
NlaIII NlaIV PleI Sau96I ScrFI
SfaNI SmaI TseI XmaCI XmaI