Restriction map for
pF1KB8403
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspHI AvaI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BfrI BglI BglII BlnI BsaAI
BsaBI BsaI BsaJI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI
BstUI BstXI Bsu36I CfoI Cfr10I
ClaI CpoI DraIII DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoRV EcoT14I EcoT22I
FseI FspI HaeII HaeIII HgaI
HhaI HinP1I HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NcoI NgoMIV NheI
NlaIV NotI NruI NsiI PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PstI PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI StyI SwaI Van91I XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 499 GACGTC GACGT^C
AccI 469 GTATAC GT^[AC][GT]AC
AcyI 499 GACGTC G[GA]^CG[CT]C
AspI 453 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 574 GGGCTC G[GA]GC[CT]^C
BbsI 289 GAAGAC GAAGAC(2/6)
BclI 48 TGATCA T^GATCA
BlpI 832 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 832 GCTCAGC GC^T[ACGT]AGC
BpuAI 289 GAAGAC GAAGAC(2/6)
BsaHI 499 GACGTC G[GA]^CG[CT]C
BseRI 1622 GAGGAG GAGGAG(10/8)
BsiYI 718 CCTGGTATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 718 CCTGGTATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 1117 GGGAC GGGAC(10/14)
Bsp1407I 931 TGTACA T^GTACA
BsrGI 931 TGTACA T^GTACA
Bst1107I 469 GTATAC GTA^TAC
BstBI 1172 TTCGAA TT^CGAA
BstEII 189 GGTTACC G^GT[ACGT]ACC
BstF5I 77 CATCC GGATG(2/0)
BstNI 718 CCTGG CC^[AT]GG
BstPI 189 GGTTACC G^GT[ACGT]ACC
BstZ17I 469 GTATAC GTA^TAC
CelII 832 GCTCAGC GC^T[ACGT]AGC
DraII 1833 AGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 1507 GACCAAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 1833 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 189 GGTTACC G^GT[ACGT]ACC
EcoRI 1474 GAATTC G^AATTC
EcoRII 718 CCTGG ^CC[AT]GG
FbaI 48 TGATCA T^GATCA
FokI 77 CATCC GGATG(9/13)
Hin1I 499 GACGTC G[GA]^CG[CT]C
MslI 1341 CACTCATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 424 CAGCTG C[AC]G^C[GT]G
MvaI 718 CCTGG CC^[AT]GG
NdeI 1345 CATATG CA^TATG
NspV 1172 TTCGAA TT^CGAA
PflFI 453 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1833 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 424 CAGCTG CAG^CTG
SexAI 717 ACCTGGT A^CC[AT]GGT
SfuI 1172 TTCGAA TT^CGAA
SspBI 931 TGTACA T^GTACA
SspI 179 AATATT AAT^ATT
TaqI 1173 TCGA T^CGA
Tsp45I 451 GTGAC ^GT[GC]AC
Tth111I 453 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1173 TCGA T^CGA
XbaI 1618 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AlwNI 421 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2009 CAGATTCTG
AseI 1029 ATTAAT AT^TAAT
1468 ATTAAT
AsnI 1029 ATTAAT AT^TAAT
1468 ATTAAT
Asp700I 1351 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1573 GAAAAATTTC
AvaII 889 GGACC G^G[AT]CC
1834 GGACC
BcgI 25 GCAGCCAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1059 GCACTATGGTCG
BciVI 839 GTATCC GTATCC(6/5)
1705 GTATCC
BcnI 1380 CCGGG CC^[GC]GG
1729 CCGGG
BmyI 574 GGGCTC G[AGT]GC[ACT]^C
1083 GTGCCC
BpmI 1929 CTCCAG CTGGAG(16/14)
590 CTGGAG
Bsp1286I 574 GGGCTC G[AGT]GC[ACT]^C
1083 GTGCCC
BspHI 45 TCATGA T^CATGA
1980 TCATGA
BspMI 193 ACCTGC ACCTGC(4/8)
491 ACCTGC
Cfr13I 889 GGACC G^G[ACGT]CC
1834 GGACC
EarI 411 GAAGAG CTCTTC(1/4)
2109 GAAGAG
FauI 299 CCCGC CCCGC(4/6)
549 GCGGG
HapII 1380 CCGG C^CGG
1729 CCGG
HincII 677 GTCAAC GT[CT]^[GA]AC
1504 GTTGAC
HindII 677 GTCAAC GT[CT]^[GA]AC
1504 GTTGAC
HpaII 1380 CCGG C^CGG
1729 CCGG
Ksp632I 411 GAAGAG CTCTTC(1/4)
2109 GAAGAG
MaeII 14 ACGT A^CGT
500 ACGT
MspI 1380 CCGG C^CGG
1729 CCGG
NciI 1380 CCGGG CC^[GC]GG
1729 CCGGG
PshBI 1029 ATTAAT AT^TAAT
1468 ATTAAT
RcaI 45 TCATGA T^CATGA
1980 TCATGA
Sau96I 889 GGACC G^G[ACGT]CC
1834 GGACC
SmlI 1847 CTCAAG C^T[CT][GA]AG
2054 CTCAAG
XmnI 1351 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1573 GAAAAATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI BbvI BfaI BsmAI
BspLU11I BsrI BssKI BstYI Cac8I
DdeI DpnII DraI Fnu4HI HinfI
HphI ItaI MaeI MaeIII MboI
MboII MflI MnlI MseI MwoI
NdeII NlaIII NspI PleI RsaI
Sau3AI ScrFI SfaNI SfcI TfiI
Tru9I TseI Tsp509I TspRI XhoII