Restriction map for
pF1KB8407
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AseI AsnI Asp700I Asp718I
AspEI AvaI AviII AvrII BalI
BamHI BanII BbeI BbrPI BbvCI
BcgI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BseAI BseRI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspMI BsrDI BsrGI BssSI
Bst1107I BstBI BstEII BstPI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraII Eam1105I Eco47III Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI MaeII MamI
MfeI MflI MluI MluNI MroI
MscI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tsp509I TthHB8I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AflIII 727 ACATGT A^C[GA][CT]GT
AlwI 186 GATCC GGATC(4/5)
AscI 120 GGCGCGCC GG^CGCGCC
AspHI 178 GAGCAC G[AT]GC[AT]^C
AspI 586 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 678 GGACC G^G[AT]CC
BanI 148 GGTGCC G^G[CT][GA]CC
BciVI 6 GTATCC GTATCC(6/5)
BcnI 50 CCGGG CC^[GC]GG
BpmI 76 CTGGAG CTGGAG(16/14)
BsaI 637 GAGACC GGTCTC(1/5)
BsaWI 145 TCCGGT [AT]^CCGG[AT]
BsgI 25 GTGCAG GTGCAG(16/14)
BsiEI 47 CGGCCG CG[GA][CT]^CG
BsiHKAI 178 GAGCAC G[AT]GC[AT]^C
BsmFI 255 GTCCC GGGAC(10/14)
BspLU11I 727 ACATGT A^CATGT
BsrBI 44 GAGCGG CCGCTC(-3/-3)
BssHII 121 GCGCGC G^CGCGC
BstAPI 560 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 818 CATCC GGATG(2/0)
BstXI 503 CCACTAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 216 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 679 GACCCTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 47 CGGCCG C^GGCCG
EclXI 47 CGGCCG C^GGCCG
Eco52I 47 CGGCCG C^GGCCG
FauI 10 CCCGC CCCGC(4/6)
FokI 818 CATCC GGATG(9/13)
KspI 32 CCGCGG CCGC^GG
MslI 504 CACTAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 15 GCCGGC GCC^GGC
NciI 50 CCGGG CC^[GC]GG
NcoI 321 CCATGG C^CATGG
NgoMIV 15 GCCGGC G^CCGGC
PflFI 586 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 558 CTGCAG CTGCA^G
PvuII 701 CAGCTG CAG^CTG
SacII 32 CCGCGG CCGC^GG
SapI 372 GAAGAGC GCTCTTC(1/4)
SfaNI 814 GCATC GCATC(5/9)
SfcI 558 CTGCAG C^T[GA][CT]AG
TfiI 831 GATTC G^A[AT]TC
Tth111I 586 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 453 GTAC GT^AC
759 GTAC
AlwNI 259 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
561 CAGCCTCTG
BbsI 465 GAAGAC GAAGAC(2/6)
877 GAAGAC
BfaI 313 CTAG C^TAG
358 CTAG
BmyI 149 GTGCCC G[AGT]GC[ACT]^C
178 GAGCAC
BpuAI 465 GAAGAC GAAGAC(2/6)
877 GAAGAC
Bsp1286I 149 GTGCCC G[AGT]GC[ACT]^C
178 GAGCAC
BsrFI 15 GCCGGC [GA]^CCGG[CT]
156 GCCGGT
Cfr10I 15 GCCGGC [GA]^CCGG[CT]
156 GCCGGT
DpnII 186 GATC ^GATC
367 GATC
EaeI 17 CGGCCA [CT]^GGCC[GA]
47 CGGCCG
EcoT14I 321 CCATGG C^C[AT][AT]GG
872 CCTTGG
HphI 209 TCACC GGTGA(8/7)
799 TCACC
MaeI 313 CTAG C^TAG
358 CTAG
MboI 186 GATC ^GATC
367 GATC
MseI 337 TTAA T^TAA
484 TTAA
NdeII 186 GATC ^GATC
367 GATC
NspI 288 ACATGC [GA]CATG^[CT]
727 ACATGT
RsaI 453 GTAC GT^AC
759 GTAC
Sau3AI 186 GATC ^GATC
367 GATC
StyI 321 CCATGG C^C[AT][AT]GG
872 CCTTGG
Tru9I 337 TTAA T^TAA
484 TTAA
Tsp45I 688 GTCAC ^GT[GC]AC
798 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AccII AciI AluI BbvI BsaJI
BsiYI BslI BsmAI BsrI BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DsaI EarI EcoRII Fnu4HI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI Ksp632I MaeIII MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NlaIII NlaIV PflMI PleI
Sau96I ScrFI TseI TspRI Van91I