Restriction map for
pF1KB8410
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# NO CUT ENZYMES ( 156 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BalI BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaJI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
HaeII Hin1I HincII HindII HindIII
HpaI HphI KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MseI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tru9I Tth111I XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AflIII 396 ACATGT A^C[GA][CT]GT
AlwNI 182 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 40 CTCGAG C^[CT]CG[GA]G
AviII 176 TGCGCA TGC^GCA
BamHI 746 GGATCC G^GATCC
BanII 576 GAGCTC G[GA]GC[CT]^C
BcnI 498 CCGGG CC^[GC]GG
BfaI 943 CTAG C^TAG
BseRI 724 GAGGAG GAGGAG(10/8)
BsmFI 549 GTCCC GGGAC(10/14)
BsoBI 40 CTCGAG C^[CT]CG[GA]G
BspLU11I 396 ACATGT A^CATGT
BspMI 9 GCAGGT ACCTGC(4/8)
BssSI 563 CACGAG CACGAG(-5/-1)
BstNI 720 CCAGG CC^[AT]GG
CfoI 177 GCGC GCG^C
EaeI 495 TGGCCG [CT]^GGCC[GA]
EcoNI 714 CCTGTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 720 CCAGG ^CC[AT]GG
FauI 839 GCGGG CCCGC(4/6)
FspI 176 TGCGCA TGC^GCA
HapII 498 CCGG C^CGG
HgaI 49 GACGC GACGC(5/10)
HhaI 177 GCGC GCG^C
HinP1I 177 GCGC G^CGC
HpaII 498 CCGG C^CGG
MaeI 943 CTAG C^TAG
MaeII 243 ACGT A^CGT
MslI 397 CATGTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 498 CCGG C^CGG
MvaI 720 CCAGG CC^[AT]GG
NciI 498 CCGGG CC^[GC]GG
NlaIV 746 GGATCC GG[ACGT]^[ACGT]CC
NspI 396 ACATGT [GA]CATG^[CT]
PaeR7I 40 CTCGAG C^TCGAG
PflMI 426 CCAGATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 293 CTGCAG CTGCA^G
PvuII 286 CAGCTG CAG^CTG
SacI 576 GAGCTC GAGCT^C
SapI 916 GAAGAGC GCTCTTC(1/4)
SmlI 40 CTCGAG C^T[CT][GA]AG
Van91I 426 CCAGATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 942 TCTAGA T^CTAGA
XhoI 40 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 151 AAATTT [GA]^AATT[CT]
272 AAATTT
ApoI 151 AAATTT [GA]^AATT[CT]
272 AAATTT
AspHI 81 GAGCAC G[AT]GC[AT]^C
576 GAGCTC
AvaII 261 GGACC G^G[AT]CC
559 GGTCC
BmyI 81 GAGCAC G[AGT]GC[ACT]^C
576 GAGCTC
BsiHKAI 81 GAGCAC G[AT]GC[AT]^C
576 GAGCTC
BsiYI 426 CCAGATGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
714 CCTGTGCCAGG
BslI 426 CCAGATGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
714 CCTGTGCCAGG
BsmAI 566 GAGAC GTCTC(1/5)
65 GTCTC
Bsp1286I 81 GAGCAC G[AGT]GC[ACT]^C
576 GAGCTC
BssKI 498 CCGGG ^CC[ACGT]GG
720 CCAGG
BstAPI 9 GCAGGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
18 GCAGGCGGTGC
BstF5I 370 CATCC GGATG(2/0)
732 GGATG
EarI 613 GAAGAG CTCTTC(1/4)
916 GAAGAG
FokI 370 CATCC GGATG(9/13)
732 GGATG
Ksp632I 613 GAAGAG CTCTTC(1/4)
916 GAAGAG
MspA1I 286 CAGCTG C[AC]G^C[GT]G
837 CAGCGG
ScrFI 498 CCGGG CC^[ACGT]GG
720 CCAGG
SfaNI 199 GATGC GCATC(5/9)
733 GATGC
SfcI 293 CTGCAG C^T[GA][CT]AG
850 CTACAG
TaqI 41 TCGA T^CGA
635 TCGA
Tsp45I 143 GTGAC ^GT[GC]AC
664 GTCAC
TspRI 797 CAGTG CA[GC]TG[ACGT][ACGT]^
853 CAGTG
TthHB8I 41 TCGA T^CGA
635 TCGA
XcmI 341 CCATGAGAGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
562 CCACGAGACTTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BpmI BsgI BsrI BstYI Cac8I
Cfr13I DdeI DpnII Fnu4HI HaeIII
HinfI ItaI MaeIII MboI MboII
MflI MnlI MwoI NdeII NlaIII
PleI RsaI Sau3AI Sau96I TfiI
TseI Tsp509I XhoII