Restriction map for
pF1KB8419
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# NO CUT ENZYMES ( 172 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AviII
AvrII BalI BamHI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaHI BseAI BsgI BsiEI
BsiHKAI BsiWI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HpaI KasI
KpnI KspI MaeI MamI MfeI
MflI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI Tth111I
TthHB8I XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AccI 382 GTAGAC GT^[AC][GT]AC
AvaI 655 CCCGAG C^[CT]CG[GA]G
BanI 1370 GGCACC G^G[CT][GA]CC
BmyI 819 GTGCCC G[AGT]GC[ACT]^C
BpmI 800 CTGGAG CTGGAG(16/14)
BsaAI 1468 CACGTA [CT]AC^GT[GA]
BsaI 24 GGTCTC GGTCTC(1/5)
BsaJI 1277 CCCTGG C^C[ACGT][ACGT]GG
BsaWI 1373 ACCGGA [AT]^CCGG[AT]
BseRI 304 GAGGAG GAGGAG(10/8)
BsmBI 1569 CGTCTC CGTCTC(1/5)
BsoBI 655 CCCGAG C^[CT]CG[GA]G
Bsp1286I 819 GTGCCC G[AGT]GC[ACT]^C
BsrDI 1106 GCAATG GCAATG(2/0)
BstXI 214 CCACTGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII 707 GATC ^GATC
EcoNI 70 CCTTTCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 369 GAATTC G^AATTC
HaeIII 1180 GGCC GG^CC
HapII 1374 CCGG C^CGG
HpaII 1374 CCGG C^CGG
MboI 707 GATC ^GATC
MspI 1374 CCGG C^CGG
NdeI 728 CATATG CA^TATG
NdeII 707 GATC ^GATC
PflMI 991 CCAGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 1413 CAGCTG CAG^CTG
Sau3AI 707 GATC ^GATC
SmlI 907 CTTGAG C^T[CT][GA]AG
Tsp45I 761 GTCAC ^GT[GC]AC
Van91I 991 CCAGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 30 enzymes) #
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AciI 746 GCGG CCGC(-3/-1)
873 GCGG
AfaI 443 GTAC GT^AC
1409 GTAC
AvaII 455 GGACC G^G[AT]CC
1379 GGACC
BbvI 677 GCAGC GCAGC(8/12)
680 GCAGC
BsmFI 332 GGGAC GGGAC(10/14)
653 GTCCC
BsmI 539 GAATGC GAATGC(1/-1)
1120 GAATGC
BspHI 246 TCATGA T^CATGA
1512 TCATGA
BssKI 324 CCAGG ^CC[ACGT]GG
1278 CCTGG
BstF5I 374 CATCC GGATG(2/0)
198 GGATG
BstNI 324 CCAGG CC^[AT]GG
1278 CCTGG
Cfr13I 455 GGACC G^G[ACGT]CC
1379 GGACC
DraII 454 AGGACCC [GA]G^G[ACGT]CC[CT]
1378 AGGACCC
EcoO109I 454 AGGACCC [GA]G^G[ACGT]CC[CT]
1378 AGGACCC
EcoRII 324 CCAGG ^CC[AT]GG
1278 CCTGG
Fnu4HI 677 GCAGC GC^[ACGT]GC
680 GCAGC
FokI 374 CATCC GGATG(9/13)
198 GGATG
ItaI 677 GCAGC GC^[ACGT]GC
680 GCAGC
MaeII 1469 ACGT A^CGT
1496 ACGT
MaeIII 509 GTAAC ^GT[ACGT]AC
761 GTCAC
MseI 140 TTAA T^TAA
1358 TTAA
MspA1I 871 CAGCGG C[AC]G^C[GT]G
1413 CAGCTG
MvaI 324 CCAGG CC^[AT]GG
1278 CCTGG
MwoI 746 GCGGAAAAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
857 GCCTCATTCGC
PpuMI 454 AGGACCC [GA]G^G[AT]CC[CT]
1378 AGGACCC
RcaI 246 TCATGA T^CATGA
1512 TCATGA
RsaI 443 GTAC GT^AC
1409 GTAC
Sau96I 455 GGACC G^G[ACGT]CC
1379 GGACC
ScrFI 324 CCAGG CC^[ACGT]GG
1278 CCTGG
Tru9I 140 TTAA T^TAA
1358 TTAA
TseI 677 GCAGC G^C[AT]GC
680 GCAGC
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# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AcsI AluI ApoI Asp700I BsiYI
BslI BsmAI BsrI DdeI EarI
HindIII HinfI HphI Ksp632I MboII
MnlI MslI NlaIII NlaIV PleI
SfaNI TfiI Tsp509I TspRI XmnI