################################## # NO CUT ENZYMES ( 152 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BcgI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaWI BseAI BsgI BsiHKAI BsiWI BsmBI BsmFI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BssSI Bst1107I BstBI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII Eam1105I Eco47III Eco81I EcoNI EcoRI EcoT14I EcoT22I FauI FseI FspI HaeII HgaI Hin1I HindIII KasI KpnI MaeII MfeI MflI MluI MluNI MroI MscI MslI MunI NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AlwNI 65 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG BbvCI 603 GCTGAGG CCTCAGC(-5/-2) BbvI 589 GCTGC GCAGC(8/12) BciVI 201 GGATAC GTATCC(6/5) BclI 359 TGATCA T^GATCA BcnI 260 CCGGG CC^[GC]GG BfaI 340 CTAG C^TAG BpmI 423 CTCCAG CTGGAG(16/14) BsaBI 391 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 268 CTCCTC GAGGAG(10/8) BsiEI 257 CGGCCG CG[GA][CT]^CG BsiYI 249 CCGAGGCGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 249 CCGAGGCGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 597 GAGAC GTCTC(1/5) BsmI 335 GAATGC GAATGC(1/-1) Bsp1407I 501 TGTACA T^GTACA BsrGI 501 TGTACA T^GTACA BsrI 70 ACTGG ACTGG(1/-1) BssHII 618 GCGCGC G^CGCGC BstAPI 74 GCAAATCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 608 GGTCACC G^GT[ACGT]ACC BstNI 387 CCTGG CC^[AT]GG BstPI 608 GGTCACC G^GT[ACGT]ACC DrdI 9 GACGGCCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 613 CCGCGG C^C[GA][CT]GG EagI 257 CGGCCG C^GGCCG EarI 490 GAAGAG CTCTTC(1/4) EclXI 257 CGGCCG C^GGCCG Eco52I 257 CGGCCG C^GGCCG EcoO65I 608 GGTCACC G^GT[ACGT]ACC EcoRII 387 CCTGG ^CC[AT]GG EcoRV 280 GATATC GAT^ATC FbaI 359 TGATCA T^GATCA HapII 260 CCGG C^CGG HincII 321 GTTAAC GT[CT]^[GA]AC HindII 321 GTTAAC GT[CT]^[GA]AC HpaI 321 GTTAAC GTT^AAC HpaII 260 CCGG C^CGG Ksp632I 490 GAAGAG CTCTTC(1/4) KspI 613 CCGCGG CCGC^GG MaeI 340 CTAG C^TAG MamI 391 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboII 490 GAAGA GAAGA(8/7) MspI 260 CCGG C^CGG MvaI 387 CCTGG CC^[AT]GG NciI 260 CCGGG CC^[GC]GG NheI 339 GCTAGC G^CTAGC NlaIII 15 CATG CATG^ PleI 121 GACTC GAGTC(4/5) PpuMI 383 AGGACCT [GA]G^G[AT]CC[CT] PvuII 601 CAGCTG CAG^CTG SacII 613 CCGCGG CCGC^GG SspBI 501 TGTACA T^GTACA TaqI 533 TCGA T^CGA TseI 589 GCTGC G^C[AT]GC TspRI 505 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 533 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcsI 109 AAATTC [GA]^AATT[CT] 145 GAATTT AfaI 502 GTAC GT^AC 579 GTAC ApoI 109 AAATTC [GA]^AATT[CT] 145 GAATTT BanII 263 GGGCTC G[GA]GC[CT]^C 564 GGGCTC BmyI 263 GGGCTC G[AGT]GC[ACT]^C 564 GGGCTC Bsp1286I 263 GGGCTC G[AGT]GC[ACT]^C 564 GGGCTC BssKI 260 CCGGG ^CC[ACGT]GG 387 CCTGG BstF5I 363 CATCC GGATG(2/0) 390 GGATG DpnII 360 GATC ^GATC 397 GATC DraII 383 AGGACCT [GA]G^G[ACGT]CC[CT] 626 AGGCCCC EaeI 11 CGGCCA [CT]^GGCC[GA] 257 CGGCCG EcoO109I 383 AGGACCT [GA]G^G[ACGT]CC[CT] 626 AGGCCCC FokI 363 CATCC GGATG(9/13) 390 GGATG MboI 360 GATC ^GATC 397 GATC MspA1I 601 CAGCTG C[AC]G^C[GT]G 613 CCGCGG NdeII 360 GATC ^GATC 397 GATC NlaIV 549 GGACCC GG[ACGT]^[ACGT]CC 627 GGCCCC RsaI 502 GTAC GT^AC 579 GTAC Sau3AI 360 GATC ^GATC 397 GATC ScrFI 260 CCGGG CC^[ACGT]GG 387 CCTGG SmlI 434 CTCAAG C^T[CT][GA]AG 594 CTTGAG TfiI 221 GATTC G^A[AT]TC 535 GAATC Tsp45I 232 GTGAC ^GT[GC]AC 609 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 26 enzymes) # ######################################### AccII AciI AluI AvaII BsaJI BstUI Cac8I CfoI Cfr13I DdeI Fnu4HI HaeIII HhaI HinP1I HinfI HphI ItaI MaeIII MnlI MseI MvnI MwoI Sau96I SfaNI Tru9I Tsp509I