Restriction map for
pF1KB8426
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# NO CUT ENZYMES ( 152 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbsI BcgI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaWI
BseAI BsgI BsiHKAI BsiWI BsmBI
BsmFI BsoBI BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BssSI Bst1107I BstBI BstXI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII Eam1105I Eco47III
Eco81I EcoNI EcoRI EcoT14I EcoT22I
FauI FseI FspI HaeII HgaI
Hin1I HindIII KasI KpnI MaeII
MfeI MflI MluI MluNI MroI
MscI MslI MunI NaeI NarI
NcoI NdeI NgoMIV NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI StyI SwaI Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AlwNI 65 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
BbvCI 603 GCTGAGG CCTCAGC(-5/-2)
BbvI 589 GCTGC GCAGC(8/12)
BciVI 201 GGATAC GTATCC(6/5)
BclI 359 TGATCA T^GATCA
BcnI 260 CCGGG CC^[GC]GG
BfaI 340 CTAG C^TAG
BpmI 423 CTCCAG CTGGAG(16/14)
BsaBI 391 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 268 CTCCTC GAGGAG(10/8)
BsiEI 257 CGGCCG CG[GA][CT]^CG
BsiYI 249 CCGAGGCGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 249 CCGAGGCGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 597 GAGAC GTCTC(1/5)
BsmI 335 GAATGC GAATGC(1/-1)
Bsp1407I 501 TGTACA T^GTACA
BsrGI 501 TGTACA T^GTACA
BsrI 70 ACTGG ACTGG(1/-1)
BssHII 618 GCGCGC G^CGCGC
BstAPI 74 GCAAATCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 608 GGTCACC G^GT[ACGT]ACC
BstNI 387 CCTGG CC^[AT]GG
BstPI 608 GGTCACC G^GT[ACGT]ACC
DrdI 9 GACGGCCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 613 CCGCGG C^C[GA][CT]GG
EagI 257 CGGCCG C^GGCCG
EarI 490 GAAGAG CTCTTC(1/4)
EclXI 257 CGGCCG C^GGCCG
Eco52I 257 CGGCCG C^GGCCG
EcoO65I 608 GGTCACC G^GT[ACGT]ACC
EcoRII 387 CCTGG ^CC[AT]GG
EcoRV 280 GATATC GAT^ATC
FbaI 359 TGATCA T^GATCA
HapII 260 CCGG C^CGG
HincII 321 GTTAAC GT[CT]^[GA]AC
HindII 321 GTTAAC GT[CT]^[GA]AC
HpaI 321 GTTAAC GTT^AAC
HpaII 260 CCGG C^CGG
Ksp632I 490 GAAGAG CTCTTC(1/4)
KspI 613 CCGCGG CCGC^GG
MaeI 340 CTAG C^TAG
MamI 391 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 490 GAAGA GAAGA(8/7)
MspI 260 CCGG C^CGG
MvaI 387 CCTGG CC^[AT]GG
NciI 260 CCGGG CC^[GC]GG
NheI 339 GCTAGC G^CTAGC
NlaIII 15 CATG CATG^
PleI 121 GACTC GAGTC(4/5)
PpuMI 383 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 601 CAGCTG CAG^CTG
SacII 613 CCGCGG CCGC^GG
SspBI 501 TGTACA T^GTACA
TaqI 533 TCGA T^CGA
TseI 589 GCTGC G^C[AT]GC
TspRI 505 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 533 TCGA T^CGA
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 109 AAATTC [GA]^AATT[CT]
145 GAATTT
AfaI 502 GTAC GT^AC
579 GTAC
ApoI 109 AAATTC [GA]^AATT[CT]
145 GAATTT
BanII 263 GGGCTC G[GA]GC[CT]^C
564 GGGCTC
BmyI 263 GGGCTC G[AGT]GC[ACT]^C
564 GGGCTC
Bsp1286I 263 GGGCTC G[AGT]GC[ACT]^C
564 GGGCTC
BssKI 260 CCGGG ^CC[ACGT]GG
387 CCTGG
BstF5I 363 CATCC GGATG(2/0)
390 GGATG
DpnII 360 GATC ^GATC
397 GATC
DraII 383 AGGACCT [GA]G^G[ACGT]CC[CT]
626 AGGCCCC
EaeI 11 CGGCCA [CT]^GGCC[GA]
257 CGGCCG
EcoO109I 383 AGGACCT [GA]G^G[ACGT]CC[CT]
626 AGGCCCC
FokI 363 CATCC GGATG(9/13)
390 GGATG
MboI 360 GATC ^GATC
397 GATC
MspA1I 601 CAGCTG C[AC]G^C[GT]G
613 CCGCGG
NdeII 360 GATC ^GATC
397 GATC
NlaIV 549 GGACCC GG[ACGT]^[ACGT]CC
627 GGCCCC
RsaI 502 GTAC GT^AC
579 GTAC
Sau3AI 360 GATC ^GATC
397 GATC
ScrFI 260 CCGGG CC^[ACGT]GG
387 CCTGG
SmlI 434 CTCAAG C^T[CT][GA]AG
594 CTTGAG
TfiI 221 GATTC G^A[AT]TC
535 GAATC
Tsp45I 232 GTGAC ^GT[GC]AC
609 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AccII AciI AluI AvaII BsaJI
BstUI Cac8I CfoI Cfr13I DdeI
Fnu4HI HaeIII HhaI HinP1I HinfI
HphI ItaI MaeIII MnlI MseI
MvnI MwoI Sau96I SfaNI Tru9I
Tsp509I