Restriction map for pF1KB8426
##################################
# NO CUT ENZYMES (  152 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanI        
BbeI        BbrPI       BbsI        BcgI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaAI       BsaHI       BsaI        BsaWI       
BseAI       BsgI        BsiHKAI     BsiWI       BsmBI       
BsmFI       BsoBI       BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BssSI       Bst1107I    BstBI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraI        DraIII      Eam1105I    Eco47III    
Eco81I      EcoNI       EcoRI       EcoT14I     EcoT22I     
FauI        FseI        FspI        HaeII       HgaI        
Hin1I       HindIII     KasI        KpnI        MaeII       
MfeI        MflI        MluI        MluNI       MroI        
MscI        MslI        MunI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacI        SalI        
SapI        SbfI        ScaI        SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        StyI        SwaI        Tth111I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

AlwNI          65  CAGGAACTG            CAG[ACGT][ACGT][ACGT]^CTG
BbvCI         603  GCTGAGG              CCTCAGC(-5/-2)
BbvI          589  GCTGC                GCAGC(8/12)
BciVI         201  GGATAC               GTATCC(6/5)
BclI          359  TGATCA               T^GATCA
BcnI          260  CCGGG                CC^[GC]GG
BfaI          340  CTAG                 C^TAG
BpmI          423  CTCCAG               CTGGAG(16/14)
BsaBI         391  GATGCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI         268  CTCCTC               GAGGAG(10/8)
BsiEI         257  CGGCCG               CG[GA][CT]^CG
BsiYI         249  CCGAGGCGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          249  CCGAGGCGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         597  GAGAC                GTCTC(1/5)
BsmI          335  GAATGC               GAATGC(1/-1)
Bsp1407I      501  TGTACA               T^GTACA
BsrGI         501  TGTACA               T^GTACA
BsrI           70  ACTGG                ACTGG(1/-1)
BssHII        618  GCGCGC               G^CGCGC
BstAPI         74  GCAAATCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        608  GGTCACC              G^GT[ACGT]ACC
BstNI         387  CCTGG                CC^[AT]GG
BstPI         608  GGTCACC              G^GT[ACGT]ACC
DrdI            9  GACGGCCATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          613  CCGCGG               C^C[GA][CT]GG
EagI          257  CGGCCG               C^GGCCG
EarI          490  GAAGAG               CTCTTC(1/4)
EclXI         257  CGGCCG               C^GGCCG
Eco52I        257  CGGCCG               C^GGCCG
EcoO65I       608  GGTCACC              G^GT[ACGT]ACC
EcoRII        387  CCTGG                ^CC[AT]GG
EcoRV         280  GATATC               GAT^ATC
FbaI          359  TGATCA               T^GATCA
HapII         260  CCGG                 C^CGG
HincII        321  GTTAAC               GT[CT]^[GA]AC
HindII        321  GTTAAC               GT[CT]^[GA]AC
HpaI          321  GTTAAC               GTT^AAC
HpaII         260  CCGG                 C^CGG
Ksp632I       490  GAAGAG               CTCTTC(1/4)
KspI          613  CCGCGG               CCGC^GG
MaeI          340  CTAG                 C^TAG
MamI          391  GATGCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII         490  GAAGA                GAAGA(8/7)
MspI          260  CCGG                 C^CGG
MvaI          387  CCTGG                CC^[AT]GG
NciI          260  CCGGG                CC^[GC]GG
NheI          339  GCTAGC               G^CTAGC
NlaIII         15  CATG                 CATG^
PleI          121  GACTC                GAGTC(4/5)
PpuMI         383  AGGACCT              [GA]G^G[AT]CC[CT]
PvuII         601  CAGCTG               CAG^CTG
SacII         613  CCGCGG               CCGC^GG
SspBI         501  TGTACA               T^GTACA
TaqI          533  TCGA                 T^CGA
TseI          589  GCTGC                G^C[AT]GC
TspRI         505  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       533  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AcsI          109  AAATTC               [GA]^AATT[CT]
              145  GAATTT              
AfaI          502  GTAC                 GT^AC
              579  GTAC                
ApoI          109  AAATTC               [GA]^AATT[CT]
              145  GAATTT              
BanII         263  GGGCTC               G[GA]GC[CT]^C
              564  GGGCTC              
BmyI          263  GGGCTC               G[AGT]GC[ACT]^C
              564  GGGCTC              
Bsp1286I      263  GGGCTC               G[AGT]GC[ACT]^C
              564  GGGCTC              
BssKI         260  CCGGG                ^CC[ACGT]GG
              387  CCTGG               
BstF5I        363  CATCC                GGATG(2/0)
              390  GGATG               
DpnII         360  GATC                 ^GATC
              397  GATC                
DraII         383  AGGACCT              [GA]G^G[ACGT]CC[CT]
              626  AGGCCCC             
EaeI           11  CGGCCA               [CT]^GGCC[GA]
              257  CGGCCG              
EcoO109I      383  AGGACCT              [GA]G^G[ACGT]CC[CT]
              626  AGGCCCC             
FokI          363  CATCC                GGATG(9/13)
              390  GGATG               
MboI          360  GATC                 ^GATC
              397  GATC                
MspA1I        601  CAGCTG               C[AC]G^C[GT]G
              613  CCGCGG              
NdeII         360  GATC                 ^GATC
              397  GATC                
NlaIV         549  GGACCC               GG[ACGT]^[ACGT]CC
              627  GGCCCC              
RsaI          502  GTAC                 GT^AC
              579  GTAC                
Sau3AI        360  GATC                 ^GATC
              397  GATC                
ScrFI         260  CCGGG                CC^[ACGT]GG
              387  CCTGG               
SmlI          434  CTCAAG               C^T[CT][GA]AG
              594  CTTGAG              
TfiI          221  GATTC                G^A[AT]TC
              535  GAATC               
Tsp45I        232  GTGAC                ^GT[GC]AC
              609  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   26 enzymes) #
#########################################

AccII       AciI        AluI        AvaII       BsaJI       
BstUI       Cac8I       CfoI        Cfr13I      DdeI        
Fnu4HI      HaeIII      HhaI        HinP1I      HinfI       
HphI        ItaI        MaeIII      MnlI        MseI        
MvnI        MwoI        Sau96I      SfaNI       Tru9I       
Tsp509I     
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