Restriction map for
pF1KB8427
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp718I AspI
AvaI AviII AvrII BalI BanII
BbeI BbrPI BbvCI BcgI BciVI
BcnI BglI BlnI BpmI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmFI
BsoBI Bsp1407I BspDI BspEI BspHI
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI FseI
FspI HapII HgaI Hin1I HpaII
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MspI
MunI MvnI NaeI NarI NciI
NcoI NgoMIV NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Tth111I
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AflIII 2251 ACATGT A^C[GA][CT]GT
AhdI 36 GACTGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 36 GACTGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 823 GAGCAC G[AT]GC[AT]^C
AvaII 1515 GGACC G^G[AT]CC
BamHI 873 GGATCC G^GATCC
BanI 409 GGTGCC G^G[CT][GA]CC
BclI 1182 TGATCA T^GATCA
BglII 1923 AGATCT A^GATCT
BlpI 1975 GCTCAGC GC^T[ACGT]AGC
BmyI 823 GAGCAC G[AGT]GC[ACT]^C
Bpu1102I 1975 GCTCAGC GC^T[ACGT]AGC
BseRI 1754 CTCCTC GAGGAG(10/8)
BsiHKAI 823 GAGCAC G[AT]GC[AT]^C
Bsp1286I 823 GAGCAC G[AGT]GC[ACT]^C
BspLU11I 2251 ACATGT A^CATGT
BsrDI 2656 GCAATG GCAATG(2/0)
BsrI 638 CCAGT ACTGG(1/-1)
BstAPI 1654 GCAAACAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1975 GCTCAGC GC^T[ACGT]AGC
DraII 1514 AGGACCC [GA]G^G[ACGT]CC[CT]
Eam1105I 36 GACTGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 1514 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 2488 GATATC GAT^ATC
EcoT22I 1300 ATGCAT ATGCA^T
FauI 310 GCGGG CCCGC(4/6)
FbaI 1182 TGATCA T^GATCA
HaeII 982 AGCGCC [GA]GCGC^[CT]
HindIII 1494 AAGCTT A^AGCTT
MslI 948 CACTCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 931 CAGCTG C[AC]G^C[GT]G
NheI 2400 GCTAGC G^CTAGC
NsiI 1300 ATGCAT ATGCA^T
NspI 2251 ACATGT [GA]CATG^[CT]
PflMI 1047 CCAACCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1514 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 1320 GACAACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 931 CAGCTG CAG^CTG
SapI 2059 GAAGAGC GCTCTTC(1/4)
Van91I 1047 CCAACCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1209 TCTAGA T^CTAGA
XcmI 2664 CCACAGTTTAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 253 GTAGAC GT^[AC][GT]AC
2383 GTAGAC
AciI 90 GCGG CCGC(-3/-1)
310 GCGG
AcsI 395 AAATTT [GA]^AATT[CT]
2406 AAATTT
AflII 219 CTTAAG C^TTAAG
784 CTTAAG
AlwNI 1400 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
2693 CAGAAACTG
ApoI 395 AAATTT [GA]^AATT[CT]
2406 AAATTT
BfrI 219 CTTAAG C^TTAAG
784 CTTAAG
BsmI 160 GAATGC GAATGC(1/-1)
359 GCATTC
BstF5I 1113 GGATG GGATG(2/0)
1869 GGATG
Cfr13I 591 GGCCC G^G[ACGT]CC
1515 GGACC
EcoNI 481 CCTTGTCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1950 CCTTGCACAGG
EcoT14I 1250 CCAAGG C^C[AT][AT]GG
2606 CCTTGG
FokI 1113 GGATG GGATG(9/13)
1869 GGATG
HaeIII 591 GGCC GG^CC
1254 GGCC
HpaI 378 GTTAAC GTT^AAC
1269 GTTAAC
NdeI 940 CATATG CA^TATG
1361 CATATG
Sau96I 591 GGCCC G^G[ACGT]CC
1515 GGACC
ScaI 1632 AGTACT AGT^ACT
2218 AGTACT
SfcI 370 CTACAG C^T[GA][CT]AG
1665 CTATAG
StyI 1250 CCAAGG C^C[AT][AT]GG
2606 CCTTGG
TaqI 570 TCGA T^CGA
684 TCGA
TthHB8I 570 TCGA T^CGA
684 TCGA
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# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AfaI AluI AlwI AseI AsnI
Asp700I BbsI BbvI BfaI BpuAI
BsaJI BsiYI BslI BsmAI BssKI
BstNI BstYI Cac8I CfoI DdeI
DpnII EarI EcoRII Fnu4HI HhaI
HinP1I HincII HindII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
PleI PshBI RsaI Sau3AI ScrFI
SfaNI SmlI SspI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII
XmnI