Restriction map for
pF1KB8428
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AlwI AocI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AvrII BamHI BbeI BbrPI
BcgI BglI BglII BlnI BsaAI
BsaBI BsaHI BsaI BseAI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI DsaI EagI EarI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FseI HaeII Hin1I
HincII HindII HpaI KasI KpnI
Ksp632I KspI MamI MfeI MflI
MluI MroI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
PvuII RsrII SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI TfiI Van91I XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccII 12 CGCG CG^CG
AflII 637 CTTAAG C^TTAAG
AflIII 1085 ACATGT A^C[GA][CT]GT
AgeI 527 ACCGGT A^CCGGT
AhdI 940 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1081 GTGCAC G^TGCAC
ApaI 1215 GGGCCC GGGCC^C
ApaLI 1081 GTGCAC G^TGCAC
AspEI 940 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1280 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 752 TGCGCA TGC^GCA
BalI 228 TGGCCA TGG^CCA
BanI 1175 GGCACC G^G[CT][GA]CC
BbsI 565 GAAGAC GAAGAC(2/6)
BbvCI 486 CCTCAGC CCTCAGC(-5/-2)
BciVI 235 GGATAC GTATCC(6/5)
BclI 812 TGATCA T^GATCA
BfrI 637 CTTAAG C^TTAAG
BpuAI 565 GAAGAC GAAGAC(2/6)
BsmBI 694 CGTCTC CGTCTC(1/5)
BspHI 1182 TCATGA T^CATGA
BspLU11I 1085 ACATGT A^CATGT
BsrDI 325 GCAATG GCAATG(2/0)
BstAPI 1164 GCAGATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 12 CGCG CG^CG
EaeI 228 TGGCCA [CT]^GGCC[GA]
Eam1105I 940 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 812 TGATCA T^GATCA
FspI 752 TGCGCA TGC^GCA
HgaI 693 GCGTC GACGC(5/10)
HindIII 601 AAGCTT A^AGCTT
HinfI 1357 GAGTC G^A[ACGT]TC
HphI 299 TCACC GGTGA(8/7)
MluNI 228 TGGCCA TGG^CCA
MscI 228 TGGCCA TGG^CCA
MseI 638 TTAA T^TAA
MvnI 12 CGCG CG^CG
PflFI 1280 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 527 ACCGGT A^CCGGT
PleI 1357 GAGTC GAGTC(4/5)
PpuMI 797 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1352 GACGGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1182 TCATGA T^CATGA
SacI 1126 GAGCTC GAGCT^C
SexAI 1109 ACCTGGT A^CC[AT]GGT
SmaI 1196 CCCGGG CCC^GGG
SphI 155 GCATGC GCATG^C
StuI 1136 AGGCCT AGG^CCT
TaqI 1342 TCGA T^CGA
Tru9I 638 TTAA T^TAA
Tsp509I 1339 AATT ^AATT
Tth111I 1280 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1342 TCGA T^CGA
XbaI 30 TCTAGA T^CTAGA
XcmI 1304 CCATCATTGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1196 CCCGGG C^CCGGG
XmaI 1196 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AspHI 1081 GTGCAC G[AT]GC[AT]^C
1126 GAGCTC
BfaI 31 CTAG C^TAG
1210 CTAG
BpmI 1365 CTCCAG CTGGAG(16/14)
1036 CTGGAG
BsaWI 527 ACCGGT [AT]^CCGG[AT]
704 ACCGGA
BsgI 347 GTGCAG GTGCAG(16/14)
397 GTGCAG
BsiHKAI 1081 GTGCAC G[AT]GC[AT]^C
1126 GAGCTC
BsrBI 577 GAGCGG CCGCTC(-3/-3)
778 GAGCGG
DpnII 813 GATC ^GATC
1011 GATC
EcoNI 374 CCTTTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
738 CCTGCTACAGG
EcoT14I 280 CCTTGG C^C[AT][AT]GG
1295 CCAAGG
MaeI 31 CTAG C^TAG
1210 CTAG
MaeII 345 ACGT A^CGT
611 ACGT
MaeIII 221 GTGAC ^GT[ACGT]AC
958 GTCAC
MboI 813 GATC ^GATC
1011 GATC
NaeI 903 GCCGGC GCC^GGC
1260 GCCGGC
NdeII 813 GATC ^GATC
1011 GATC
NgoMIV 903 GCCGGC G^CCGGC
1260 GCCGGC
NspI 155 GCATGC [GA]CATG^[CT]
1085 ACATGT
Sau3AI 813 GATC ^GATC
1011 GATC
StyI 280 CCTTGG C^C[AT][AT]GG
1295 CCAAGG
Tsp45I 221 GTGAC ^GT[GC]AC
958 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaI
AvaII BanII BbvI BcnI BlpI
BmyI Bpu1102I BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DraII EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII ItaI MboII
MnlI MslI MspA1I MspI MvaI
MwoI NciI NlaIII NlaIV PstI
RsaI Sau96I ScrFI SfaNI SfcI
SmlI TseI TspRI