Restriction map for
pF1KB8429
##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AclI AcsI AflII AhdI ApoI
AscI AseI AsnI Asp700I AspEI
AviII AvrII BamHI BbeI BbrPI
BbsI BbvCI BciVI BclI BfaI
BfrI BglII BlnI BpuAI BsaAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BsrGI BssHII BssSI BstBI BstEII
BstPI ClaI CpoI DraI DrdI
Eam1105I EcoO65I EcoRI EcoRV EcoT22I
FbaI FspI HincII HindII HindIII
HpaI KasI KspI MaeI MfeI
MluI MslI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tsp509I TthHB8I Van91I XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AatII 967 GACGTC GACGT^C
Acc65I 1194 GGTACC G^GTACC
AccI 870 GTATAC GT^[AC][GT]AC
AccII 547 CGCG CG^CG
AccIII 920 TCCGGA T^CCGGA
AflIII 767 ACATGT A^C[GA][CT]GT
AlwI 198 GATCC GGATC(4/5)
AocI 880 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1289 AGCGCT AGC^GCT
ApaI 773 GGGCCC GGGCC^C
Asp718I 1194 GGTACC G^GTACC
AspI 538 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 655 CTCGGG C^[CT]CG[GA]G
BalI 807 TGGCCA TGG^CCA
BcgI 407 GCATCTGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 1184 CCCGG CC^[GC]GG
BglI 1276 GCCATCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 633 GATAACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 587 GAGACC GGTCTC(1/5)
BseAI 920 TCCGGA T^CCGGA
BsoBI 655 CTCGGG C^[CT]CG[GA]G
BspEI 920 TCCGGA T^CCGGA
BspHI 438 TCATGA T^CATGA
BspLU11I 767 ACATGT A^CATGT
BsrBI 247 GAGCGG CCGCTC(-3/-3)
Bst1107I 870 GTATAC GTA^TAC
BstAPI 1143 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 547 CGCG CG^CG
BstYI 197 AGATCC [GA]^GATC[CT]
BstZ17I 870 GTATAC GTA^TAC
Bsu36I 880 CCTGAGG CC^T[ACGT]AGG
DraIII 906 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1289 AGCGCT AGC^GCT
Eco81I 880 CCTGAGG CC^T[ACGT]AGG
FauI 834 CCCGC CCCGC(4/6)
FseI 739 GGCCGGCC GGCCGG^CC
HinfI 373 GAATC G^A[ACGT]TC
KpnI 1194 GGTACC GGTAC^C
MamI 633 GATAACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 197 AGATCC [GA]^GATC[CT]
MluNI 807 TGGCCA TGG^CCA
MroI 920 TCCGGA T^CCGGA
MscI 807 TGGCCA TGG^CCA
MseI 389 TTAA T^TAA
MvnI 547 CGCG CG^CG
NaeI 740 GCCGGC GCC^GGC
NciI 1184 CCCGG CC^[GC]GG
NgoMIV 740 GCCGGC G^CCGGC
PflFI 538 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 680 GGGACCT [GA]G^G[AT]CC[CT]
PstI 475 CTGCAG CTGCA^G
RcaI 438 TCATGA T^CATGA
ScaI 278 AGTACT AGT^ACT
SfcI 475 CTGCAG C^T[GA][CT]AG
TfiI 373 GAATC G^A[AT]TC
Tru9I 389 TTAA T^TAA
Tth111I 538 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1256 CCATGACCTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 197 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 967 GACGTC G[GA]^CG[CT]C
1180 GACGCC
AgeI 132 ACCGGT A^CCGGT
1224 ACCGGT
Alw44I 319 GTGCAC G^TGCAC
580 GTGCAC
ApaLI 319 GTGCAC G^TGCAC
580 GTGCAC
BanI 135 GGTGCC G^G[CT][GA]CC
1194 GGTACC
BlpI 504 GCTCAGC GC^T[ACGT]AGC
732 GCTCAGC
BpmI 208 CTGGAG CTGGAG(16/14)
973 CTGGAG
Bpu1102I 504 GCTCAGC GC^T[ACGT]AGC
732 GCTCAGC
BsaHI 967 GACGTC G[GA]^CG[CT]C
1180 GACGCC
BsiEI 738 CGGCCG CG[GA][CT]^CG
742 CGGCCG
BsrDI 993 CATTGC GCAATG(2/0)
1067 GCAATG
BstXI 50 CCAAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
377 CCAGCCGGATGG
CelII 504 GCTCAGC GC^T[ACGT]AGC
732 GCTCAGC
DpnII 198 GATC ^GATC
855 GATC
DsaI 327 CCACGG C^C[GA][CT]GG
750 CCGTGG
EagI 738 CGGCCG C^GGCCG
742 CGGCCG
EclXI 738 CGGCCG C^GGCCG
742 CGGCCG
Eco52I 738 CGGCCG C^GGCCG
742 CGGCCG
EcoNI 333 CCTCTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
684 CCTGCTGAAGG
HaeII 950 AGCGCC [GA]GCGC^[CT]
1289 AGCGCT
Hin1I 967 GACGTC G[GA]^CG[CT]C
1180 GACGCC
MaeIII 764 GTGAC ^GT[ACGT]AC
1096 GTGAC
MboI 198 GATC ^GATC
855 GATC
NdeII 198 GATC ^GATC
855 GATC
NspI 767 ACATGT [GA]CATG^[CT]
1133 ACATGC
PinAI 132 ACCGGT A^CCGGT
1224 ACCGGT
PvuII 190 CAGCTG CAG^CTG
1144 CAGCTG
SacI 841 GAGCTC GAGCT^C
1167 GAGCTC
Sau3AI 198 GATC ^GATC
855 GATC
SgrAI 131 CACCGGTG C[GA]^CCGG[CT]G
1223 CACCGGTG
Tsp45I 764 GTGAC ^GT[GC]AC
1096 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
AvaII BanII BbvI BmyI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr10I Cfr13I DdeI
DraII EaeI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HpaII HphI
ItaI Ksp632I MaeII MboII MnlI
MspA1I MspI MvaI MwoI NlaIII
NlaIV RsaI SapI Sau96I ScrFI
SfaNI StyI TseI TspRI