Restriction map for
pF1KB8433
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII AccI AccII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
AocI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BseRI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI Eam1105I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI Hin1I HincII
HindII HindIII HinfI HpaI KasI
KspI MaeI MaeII MamI MfeI
MluI MseI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tru9I Tsp509I Tth111I Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 395 GGTACC G^GTACC
AccIII 617 TCCGGA T^CCGGA
AflIII 449 ACATGT A^C[GA][CT]GT
AlwI 519 GATCC GGATC(4/5)
Aor51HI 836 AGCGCT AGC^GCT
Asp718I 395 GGTACC G^GTACC
AvaI 341 CCCGGG C^[CT]CG[GA]G
BalI 158 TGGCCA TGG^CCA
BanI 395 GGTACC G^G[CT][GA]CC
BbsI 318 GAAGAC GAAGAC(2/6)
BpuAI 318 GAAGAC GAAGAC(2/6)
BsaWI 617 TCCGGA [AT]^CCGG[AT]
BseAI 617 TCCGGA T^CCGGA
BsiEI 707 CGGCCG CG[GA][CT]^CG
BsoBI 341 CCCGGG C^[CT]CG[GA]G
BspEI 617 TCCGGA T^CCGGA
BspHI 296 TCATGA T^CATGA
BspLU11I 449 ACATGT A^CATGT
BspMI 222 GCAGGT ACCTGC(4/8)
BstXI 161 CCAGCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 518 AGATCC [GA]^GATC[CT]
DdeI 200 CTCAG C^T[ACGT]AG
DsaI 655 CCATGG C^C[GA][CT]GG
EagI 707 CGGCCG C^GGCCG
EarI 497 CTCTTC CTCTTC(1/4)
EclXI 707 CGGCCG C^GGCCG
Eco47III 836 AGCGCT AGC^GCT
Eco52I 707 CGGCCG C^GGCCG
HaeII 836 AGCGCT [GA]GCGC^[CT]
HgaI 66 GCGTC GACGC(5/10)
HphI 132 GGTGA GGTGA(8/7)
KpnI 395 GGTACC GGTAC^C
Ksp632I 497 CTCTTC CTCTTC(1/4)
MflI 518 AGATCC [GA]^GATC[CT]
MluNI 158 TGGCCA TGG^CCA
MroI 617 TCCGGA T^CCGGA
MscI 158 TGGCCA TGG^CCA
MslI 444 CATCGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 655 CCATGG C^CATGG
NspI 449 ACATGT [GA]CATG^[CT]
PstI 220 CTGCAG CTGCA^G
PvuII 544 CAGCTG CAG^CTG
RcaI 296 TCATGA T^CATGA
SfcI 220 CTGCAG C^T[GA][CT]AG
SmaI 341 CCCGGG CCC^GGG
StuI 493 AGGCCT AGG^CCT
Tsp45I 385 GTCAC ^GT[GC]AC
XcmI 587 CCAAGCTGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 518 AGATCC [GA]^GATC[CT]
XmaCI 341 CCCGGG C^CCGGG
XmaI 341 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AfaI 396 GTAC GT^AC
609 GTAC
AvaII 153 GGACC G^G[AT]CC
273 GGACC
EcoT14I 377 CCAAGG C^C[AT][AT]GG
655 CCATGG
FauI 39 GCGGG CCCGC(4/6)
801 GCGGG
MaeIII 140 GTTAC ^GT[ACGT]AC
385 GTCAC
MspA1I 323 CAGCGG C[AC]G^C[GT]G
544 CAGCTG
PpuMI 152 AGGACCT [GA]G^G[AT]CC[CT]
272 GGGACCT
RsaI 396 GTAC GT^AC
609 GTAC
SacI 726 GAGCTC GAGCT^C
766 GAGCTC
StyI 377 CCAAGG C^C[AT][AT]GG
655 CCATGG
TaqI 446 TCGA T^CGA
530 TCGA
TspRI 561 CAGTG CA[GC]TG[ACGT][ACGT]^
596 CACTG
TthHB8I 446 TCGA T^CGA
530 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AluI AlwNI AspHI BanII
BbvI BcnI BmyI BpmI BsaJI
BsgI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DpnII DraII
EaeI EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
ItaI MboI MboII MnlI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
TseI