Restriction map for
pF1KB8437
##################################
# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI Alw44I
AlwNI AocI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspI
AviII AvrII BanI BbeI BbrPI
BcgI BciVI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspMI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII Cfr10I ClaI
DraI DraII DraIII DrdI DsaI
EagI EclXI Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tth111I Van91I
XbaI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AfaI 1122 GTAC GT^AC
AflIII 585 ACATGT A^C[GA][CT]GT
AhdI 1468 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1468 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 5 TGGCCA TGG^CCA
BamHI 1261 GGATCC G^GATCC
BanII 535 GAGCTC G[GA]GC[CT]^C
BclI 1076 TGATCA T^GATCA
BglII 750 AGATCT A^GATCT
BsaI 316 GAGACC GGTCTC(1/5)
BseRI 94 GAGGAG GAGGAG(10/8)
BsmAI 316 GAGAC GTCTC(1/5)
BsmFI 593 GTCCC GGGAC(10/14)
BspHI 444 TCATGA T^CATGA
BspLU11I 585 ACATGT A^CATGT
BsrBI 943 GAGCGG CCGCTC(-3/-3)
BstXI 596 CCATCCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 75 CGGTCCG CG^G[AT]CCG
Eam1105I 1468 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI 156 CCCGC CCCGC(4/6)
FbaI 1076 TGATCA T^GATCA
MluNI 5 TGGCCA TGG^CCA
MscI 5 TGGCCA TGG^CCA
MslI 597 CATCCTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI 141 GCTAGC G^CTAGC
PshAI 254 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1117 CAGCTG CAG^CTG
RcaI 444 TCATGA T^CATGA
RsaI 1122 GTAC GT^AC
RsrII 75 CGGTCCG CG^G[AT]CCG
SacI 535 GAGCTC GAGCT^C
SapI 532 GAAGAGC GCTCTTC(1/4)
SfaNI 342 GATGC GCATC(5/9)
StuI 437 AGGCCT AGG^CCT
XcmI 662 CCAAAGCTCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 494 AAATTT [GA]^AATT[CT]
759 GAATTT
Aor51HI 182 AGCGCT AGC^GCT
203 AGCGCT
ApoI 494 AAATTT [GA]^AATT[CT]
759 GAATTT
AspHI 282 GAGCAC G[AT]GC[AT]^C
535 GAGCTC
BbvCI 621 CCTCAGC CCTCAGC(-5/-2)
91 GCTGAGG
BmyI 282 GAGCAC G[AGT]GC[ACT]^C
535 GAGCTC
BpmI 1165 CTCCAG CTGGAG(16/14)
55 CTGGAG
BsiHKAI 282 GAGCAC G[AT]GC[AT]^C
535 GAGCTC
BsmI 925 GAATGC GAATGC(1/-1)
1207 GCATTC
Bsp1286I 282 GAGCAC G[AGT]GC[ACT]^C
535 GAGCTC
BsrI 507 ACTGG ACTGG(1/-1)
913 ACTGG
BssSI 1494 CACGAG CACGAG(-5/-1)
601 CTCGTG
BstYI 750 AGATCT [GA]^GATC[CT]
1261 GGATCC
EarI 1539 CTCTTC CTCTTC(1/4)
532 GAAGAG
Eco47III 182 AGCGCT AGC^GCT
203 AGCGCT
HaeII 182 AGCGCT [GA]GCGC^[CT]
203 AGCGCT
HgaI 88 GACGC GACGC(5/10)
332 GCGTC
Ksp632I 1539 CTCTTC CTCTTC(1/4)
532 GAAGAG
MflI 750 AGATCT [GA]^GATC[CT]
1261 GGATCC
MspA1I 72 CAGCGG C[AC]G^C[GT]G
1117 CAGCTG
NspI 585 ACATGT [GA]CATG^[CT]
1047 GCATGT
Tsp45I 133 GTCAC ^GT[GC]AC
1090 GTCAC
XhoII 750 AGATCT [GA]^GATC[CT]
1261 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AluI AlwI AvaI AvaII
BbsI BbvI BcnI BfaI BpuAI
BsaJI BsiYI BslI BsoBI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII EaeI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MnlI MseI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PaeR7I
PleI PstI Sau3AI Sau96I ScrFI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
XhoI