Restriction map for
pF1KB8441
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# NO CUT ENZYMES ( 151 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflIII AgeI
AhdI Alw44I Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BglI BglII BlnI BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV FbaI FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MamI
MfeI MluI MroI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tsp509I Tth111I Van91I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 67 enzymes) #
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AccII 224 CGCG CG^CG
AflII 340 CTTAAG C^TTAAG
AlwI 368 GATCC GGATC(4/5)
AocI 213 CCTCAGG CC^T[ACGT]AGG
ApaI 218 GGGCCC GGGCC^C
AvaI 379 CCCGGG C^[CT]CG[GA]G
BalI 248 TGGCCA TGG^CCA
BanII 218 GGGCCC G[GA]GC[CT]^C
BfrI 340 CTTAAG C^TTAAG
BlpI 450 GCTCAGC GC^T[ACGT]AGC
BmyI 218 GGGCCC G[AGT]GC[ACT]^C
Bpu1102I 450 GCTCAGC GC^T[ACGT]AGC
BsaI 40 GAGACC GGTCTC(1/5)
BsgI 463 CTGCAC GTGCAG(16/14)
BsmFI 194 GGGAC GGGAC(10/14)
BsmI 145 GAATGC GAATGC(1/-1)
BsoBI 379 CCCGGG C^[CT]CG[GA]G
Bsp1286I 218 GGGCCC G[AGT]GC[ACT]^C
BspMI 467 ACCTGC ACCTGC(4/8)
BstAPI 465 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 303 GGATG GGATG(2/0)
BstUI 224 CGCG CG^CG
BstYI 367 AGATCC [GA]^GATC[CT]
Bsu36I 213 CCTCAGG CC^T[ACGT]AGG
CelII 450 GCTCAGC GC^T[ACGT]AGC
DpnII 368 GATC ^GATC
DsaI 77 CCATGG C^C[GA][CT]GG
EaeI 248 TGGCCA [CT]^GGCC[GA]
EarI 106 GAAGAG CTCTTC(1/4)
Eco81I 213 CCTCAGG CC^T[ACGT]AGG
EcoT14I 77 CCATGG C^C[AT][AT]GG
EcoT22I 147 ATGCAT ATGCA^T
FokI 303 GGATG GGATG(9/13)
HapII 380 CCGG C^CGG
HgaI 163 GACGC GACGC(5/10)
HpaII 380 CCGG C^CGG
HphI 332 TCACC GGTGA(8/7)
Ksp632I 106 GAAGAG CTCTTC(1/4)
MaeII 206 ACGT A^CGT
MaeIII 161 GTGAC ^GT[ACGT]AC
MboI 368 GATC ^GATC
MboII 106 GAAGA GAAGA(8/7)
MflI 367 AGATCC [GA]^GATC[CT]
MluNI 248 TGGCCA TGG^CCA
MscI 248 TGGCCA TGG^CCA
MseI 341 TTAA T^TAA
MslI 30 CATCTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 380 CCGG C^CGG
MvnI 224 CGCG CG^CG
NcoI 77 CCATGG C^CATGG
NdeII 368 GATC ^GATC
NsiI 147 ATGCAT ATGCA^T
PleI 100 GACTC GAGTC(4/5)
Sau3AI 368 GATC ^GATC
SfaNI 188 GCATC GCATC(5/9)
SmaI 379 CCCGGG CCC^GGG
StyI 77 CCATGG C^C[AT][AT]GG
TaqI 134 TCGA T^CGA
TfiI 136 GATTC G^A[AT]TC
Tru9I 341 TTAA T^TAA
Tsp45I 161 GTGAC ^GT[GC]AC
TspRI 159 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 134 TCGA T^CGA
XcmI 251 CCATGCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 367 AGATCC [GA]^GATC[CT]
XmaCI 379 CCCGGG C^CCGGG
XmaI 379 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 12 enzymes) #
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AluI 59 AGCT AG^CT
236 AGCT
AlwNI 58 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
403 CAGGTGCTG
BcnI 379 CCCGG CC^[GC]GG
380 CCGGG
BpmI 21 CTCCAG CTGGAG(16/14)
363 CTGGAG
BsmAI 26 GAGAC GTCTC(1/5)
40 GAGAC
FauI 222 CCCGC CCCGC(4/6)
455 GCGGG
HaeII 414 GGCGCT [GA]GCGC^[CT]
432 GGCGCT
HinfI 100 GACTC G^A[ACGT]TC
136 GATTC
MspA1I 445 CCGCTG C[AC]G^C[GT]G
453 CAGCGG
NciI 379 CCCGG CC^[GC]GG
380 CCGGG
PpuMI 110 AGGACCT [GA]G^G[AT]CC[CT]
382 GGGTCCT
SmlI 340 CTTAAG C^T[CT][GA]AG
475 CTCAAG
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AciI AvaII BbvI BsaJI BsiYI
BslI BssKI BstNI Cac8I CfoI
Cfr13I DdeI DraII EcoO109I EcoRII
Fnu4HI HaeIII HhaI HinP1I ItaI
MnlI MvaI MwoI NlaIII NlaIV
Sau96I ScrFI TseI