Restriction map for
pF1KB8442
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# NO CUT ENZYMES ( 179 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AviII AvrII BanI
BanII BbeI BbrPI BbvCI BbvI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI BmyI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmFI Bsp1286I Bsp1407I
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
CpoI DraI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HgaI Hin1I
HincII HindII HindIII HpaI HphI
KasI KpnI KspI MaeI MaeIII
MamI MfeI MluI MroI MslI
MspA1I MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TseI Tsp45I Tsp509I
Tth111I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AluI 104 AGCT AG^CT
Asp700I 15 GAATACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 92 CCCGGG C^[CT]CG[GA]G
AvaII 207 GGACC G^G[AT]CC
BalI 168 TGGCCA TGG^CCA
BamHI 121 GGATCC G^GATCC
BbsI 33 GAAGAC GAAGAC(2/6)
BpuAI 33 GAAGAC GAAGAC(2/6)
BsaWI 209 ACCGGA [AT]^CCGG[AT]
BseRI 41 CTCCTC GAGGAG(10/8)
BsmAI 184 GAGAC GTCTC(1/5)
BsmI 72 GCATTC GAATGC(1/-1)
BsoBI 92 CCCGGG C^[CT]CG[GA]G
BspDI 83 ATCGAT AT^CGAT
BspMI 68 ACCTGC ACCTGC(4/8)
BstF5I 348 GGATG GGATG(2/0)
BstNI 138 CCTGG CC^[AT]GG
BstYI 121 GGATCC [GA]^GATC[CT]
Cac8I 233 GCAGGC GC[ACGT]^[ACGT]GC
CfoI 225 GCGC GCG^C
Cfr13I 207 GGACC G^G[ACGT]CC
ClaI 83 ATCGAT AT^CGAT
DdeI 355 CTGAG C^T[ACGT]AG
EaeI 168 TGGCCA [CT]^GGCC[GA]
EcoRII 138 CCTGG ^CC[AT]GG
Fnu4HI 273 GCGGC GC^[ACGT]GC
FokI 348 GGATG GGATG(9/13)
HaeII 224 AGCGCC [GA]GCGC^[CT]
HaeIII 169 GGCC GG^CC
HhaI 225 GCGC GCG^C
HinP1I 225 GCGC G^CGC
ItaI 273 GCGGC GC^[ACGT]GC
MaeII 19 ACGT A^CGT
MflI 121 GGATCC [GA]^GATC[CT]
MluNI 168 TGGCCA TGG^CCA
MscI 168 TGGCCA TGG^CCA
MseI 331 TTAA T^TAA
MvaI 138 CCTGG CC^[AT]GG
MwoI 237 GCGGAACCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII 300 CATG CATG^
NspI 299 ACATGC [GA]CATG^[CT]
PleI 310 GAGTC GAGTC(4/5)
Sau96I 207 GGACC G^G[ACGT]CC
SfaNI 55 GCATC GCATC(5/9)
SmaI 92 CCCGGG CCC^GGG
TaqI 84 TCGA T^CGA
TfiI 322 GAATC G^A[AT]TC
Tru9I 331 TTAA T^TAA
TthHB8I 84 TCGA T^CGA
XhoII 121 GGATCC [GA]^GATC[CT]
XmaCI 92 CCCGGG C^CCGGG
XmaI 92 CCCGGG C^CCGGG
XmnI 15 GAATACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 11 enzymes) #
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BcnI 92 CCCGG CC^[GC]GG
93 CCGGG
BsaJI 43 CCTCGG C^C[ACGT][ACGT]GG
92 CCCGGG
BsrI 345 ACTGG ACTGG(1/-1)
171 CCAGT
EarI 256 GAAGAG CTCTTC(1/4)
369 GAAGAG
HinfI 310 GAGTC G^A[ACGT]TC
322 GAATC
Ksp632I 256 GAAGAG CTCTTC(1/4)
369 GAAGAG
NciI 92 CCCGG CC^[GC]GG
93 CCGGG
NlaIV 121 GGATCC GG[ACGT]^[ACGT]CC
239 GGAACC
PflMI 243 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
327 CCACTTAATGG
TspRI 172 CAGTG CA[GC]TG[ACGT][ACGT]^
315 CACTG
Van91I 243 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
327 CCACTTAATGG
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# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AciI AlwI BsiYI BslI BssKI
DpnII HapII HpaII MboI MboII
MnlI MspI NdeII Sau3AI ScrFI