Restriction map for
pF1KB8444
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BanI BanII BbeI BbrPI
BbvCI BcgI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII ClaI DraII
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRV FbaI FseI FspI
HgaI Hin1I HincII HindII HpaI
KasI KpnI Ksp632I KspI MamI
MfeI MluI MroI MspA1I MunI
NarI NcoI NdeI NheI NotI
NruI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AluI 539 AGCT AG^CT
AlwNI 602 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
BalI 816 TGGCCA TGG^CCA
BamHI 596 GGATCC G^GATCC
BbsI 418 GAAGAC GAAGAC(2/6)
BciVI 312 GTATCC GTATCC(6/5)
BfaI 448 CTAG C^TAG
BmyI 485 GTGCCC G[AGT]GC[ACT]^C
BpuAI 418 GAAGAC GAAGAC(2/6)
BsaI 171 GGTCTC GGTCTC(1/5)
BsaWI 658 TCCGGT [AT]^CCGG[AT]
Bsp1286I 485 GTGCCC G[AGT]GC[ACT]^C
BsrBI 30 CCGCTC CCGCTC(-3/-3)
BsrI 819 CCAGT ACTGG(1/-1)
CpoI 26 CGGACCG CG^G[AT]CCG
DraI 430 TTTAAA TTT^AAA
DsaI 96 CCACGG C^C[GA][CT]GG
EcoNI 5 CCTCCGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 579 GAATTC G^AATTC
EcoT14I 764 CCTTGG C^C[AT][AT]GG
EcoT22I 614 ATGCAT ATGCA^T
FauI 168 GCGGG CCCGC(4/6)
HaeII 92 AGCGCC [GA]GCGC^[CT]
HindIII 538 AAGCTT A^AGCTT
MaeI 448 CTAG C^TAG
MaeII 869 ACGT A^CGT
MluNI 816 TGGCCA TGG^CCA
MscI 816 TGGCCA TGG^CCA
MslI 798 CACAAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 243 GCCGGC GCC^GGC
NgoMIV 243 GCCGGC G^CCGGC
NsiI 614 ATGCAT ATGCA^T
PflMI 270 CCACTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 650 CTGCAG CTGCA^G
RsrII 26 CGGACCG CG^G[AT]CCG
SfaNI 412 GATGC GCATC(5/9)
StyI 764 CCTTGG C^C[AT][AT]GG
Van91I 270 CCACTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AccII 115 CGCG CG^CG
167 CGCG
AcsI 229 GAATTT [GA]^AATT[CT]
579 GAATTC
AfaI 382 GTAC GT^AC
739 GTAC
ApoI 229 GAATTT [GA]^AATT[CT]
579 GAATTC
BbvI 673 GCAGC GCAGC(8/12)
195 GCTGC
BpmI 82 CTCCAG CTGGAG(16/14)
253 CTGGAG
BseRI 21 CTCCTC GAGGAG(10/8)
641 CTCCTC
BsrFI 51 GCCGGT [GA]^CCGG[CT]
243 GCCGGC
BstUI 115 CGCG CG^CG
167 CGCG
Cfr10I 51 GCCGGT [GA]^CCGG[CT]
243 GCCGGC
DdeI 339 CTCAG C^T[ACGT]AG
629 CTCAG
EaeI 501 TGGCCG [CT]^GGCC[GA]
816 TGGCCA
Fnu4HI 195 GCTGC GC^[ACGT]GC
673 GCAGC
ItaI 195 GCTGC GC^[ACGT]GC
673 GCAGC
MaeIII 782 GTGAC ^GT[ACGT]AC
796 GTCAC
MseI 212 TTAA T^TAA
431 TTAA
MvnI 115 CGCG CG^CG
167 CGCG
NlaIII 568 CATG CATG^
613 CATG
PleI 19 GACTC GAGTC(4/5)
387 GACTC
RsaI 382 GTAC GT^AC
739 GTAC
SfcI 356 CTACAG C^T[GA][CT]AG
650 CTGCAG
TfiI 118 GATTC G^A[AT]TC
516 GATTC
Tru9I 212 TTAA T^TAA
431 TTAA
TseI 195 GCTGC G^C[AT]GC
673 GCAGC
Tsp45I 782 GTGAC ^GT[GC]AC
796 GTCAC
Tsp509I 230 AATT ^AATT
580 AATT
TspRI 475 CAGTG CA[GC]TG[ACGT][ACGT]^
820 CAGTG
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AlwI AvaII BcnI BsaJI
BsiYI BslI BsmAI BsmFI BspMI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DpnII EcoRII FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI MboI MboII MflI
MnlI MspI MvaI MwoI NciI
NdeII NlaIV Sau3AI Sau96I ScrFI
TaqI TthHB8I XhoII