Restriction map for
pF1KB8453
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AccI AccIII AclI AcsI AflII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AspHI AspI
AviII AvrII BamHI BbeI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BsaBI BsaWI BseAI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrGI BssHII Bst1107I BstAPI
BstBI BstXI BstYI BstZ17I Bsu36I
ClaI CpoI DpnII DraI DrdI
Eam1105I EarI Eco47III Eco81I EcoNI
EcoRI EcoRV EcoT22I FbaI FseI
FspI HgaI HincII HindII HindIII
HinfI HpaI KasI Ksp632I MaeI
MamI MboI MfeI MflI MluI
MroI MunI NarI NdeI NdeII
NheI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SapI Sau3AI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tsp509I Tth111I XbaI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 490 GACGTC GACGT^C
Acc65I 74 GGTACC G^GTACC
AcyI 490 GACGTC G[GA]^CG[CT]C
AflIII 227 ACACGT A^C[GA][CT]GT
Asp718I 74 GGTACC G^GTACC
BalI 392 TGGCCA TGG^CCA
BbrPI 228 CACGTG CAC^GTG
BlpI 120 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 120 GCTGAGC GC^T[ACGT]AGC
BsaAI 228 CACGTG [CT]AC^GT[GA]
BsaHI 490 GACGTC G[GA]^CG[CT]C
BsaI 772 GAGACC GGTCTC(1/5)
BsiEI 732 CGGCCG CG[GA][CT]^CG
BsrDI 171 CATTGC GCAATG(2/0)
BssSI 558 CACGAG CACGAG(-5/-1)
CelII 120 GCTGAGC GC^T[ACGT]AGC
DraIII 310 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 732 CGGCCG C^GGCCG
EclXI 732 CGGCCG C^GGCCG
Eco52I 732 CGGCCG C^GGCCG
HaeII 450 GGCGCT [GA]GCGC^[CT]
Hin1I 490 GACGTC G[GA]^CG[CT]C
KpnI 74 GGTACC GGTAC^C
MluNI 392 TGGCCA TGG^CCA
MscI 392 TGGCCA TGG^CCA
MseI 857 TTAA T^TAA
NaeI 8 GCCGGC GCC^GGC
NcoI 701 CCATGG C^CATGG
NgoMIV 8 GCCGGC G^CCGGC
NotI 731 GCGGCCGC GC^GGCCGC
PflMI 309 CCACCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 228 CACGTG CAC^GTG
PmlI 228 CACGTG CAC^GTG
SfaNI 605 GCATC GCATC(5/9)
StuI 285 AGGCCT AGG^CCT
TaqI 380 TCGA T^CGA
Tru9I 857 TTAA T^TAA
TthHB8I 380 TCGA T^CGA
Van91I 309 CCACCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 928 CCAGATACACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AlwNI 460 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
781 CAGCTGCTG
ApaI 139 GGGCCC GGGCC^C
867 GGGCCC
BanI 74 GGTACC G^G[CT][GA]CC
955 GGTGCC
BbvCI 819 CCTCAGC CCTCAGC(-5/-2)
786 GCTGAGG
BseRI 37 CTCCTC GAGGAG(10/8)
883 GAGGAG
BsgI 696 CTGCAC GTGCAG(16/14)
794 CTGCAC
BsmAI 297 GAGAC GTCTC(1/5)
772 GAGAC
BsrFI 8 GCCGGC [GA]^CCGG[CT]
518 ACCGGC
BstEII 318 GGTCACC G^GT[ACGT]ACC
813 GGTCACC
BstF5I 525 CATCC GGATG(2/0)
678 CATCC
BstPI 318 GGTCACC G^GT[ACGT]ACC
813 GGTCACC
CfoI 207 GCGC GCG^C
451 GCGC
Cfr10I 8 GCCGGC [GA]^CCGG[CT]
518 ACCGGC
EaeI 392 TGGCCA [CT]^GGCC[GA]
732 CGGCCG
EcoO65I 318 GGTCACC G^GT[ACGT]ACC
813 GGTCACC
EcoT14I 445 CCAAGG C^C[AT][AT]GG
701 CCATGG
FokI 525 CATCC GGATG(9/13)
678 CATCC
HhaI 207 GCGC GCG^C
451 GCGC
HinP1I 207 GCGC G^CGC
451 GCGC
KspI 16 CCGCGG CCGC^GG
332 CCGCGG
MaeIII 319 GTCAC ^GT[ACGT]AC
814 GTCAC
MslI 171 CATTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
935 CACCCTGGTG
PshAI 255 GACATTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
299 GACTGGGGTC
PvuII 781 CAGCTG CAG^CTG
949 CAGCTG
SacII 16 CCGCGG CCGC^GG
332 CCGCGG
SmaI 135 CCCGGG CCC^GGG
188 CCCGGG
StyI 445 CCAAGG C^C[AT][AT]GG
701 CCATGG
Tsp45I 319 GTCAC ^GT[GC]AC
814 GTCAC
XmaCI 135 CCCGGG C^CCGGG
188 CCCGGG
XmaI 135 CCCGGG C^CCGGG
188 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AccII AciI AfaI AluI AvaI
AvaII BanII BbsI BbvI BcnI
BmyI BpmI BpuAI BsaJI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrI
BssKI BstNI BstUI Cac8I Cfr13I
DdeI DraII DsaI EcoO109I EcoRII
FauI Fnu4HI HaeIII HapII HpaII
HphI ItaI MaeII MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIII NlaIV PpuMI PstI
RsaI Sau96I ScrFI SfcI TseI
TspRI