Restriction map for
pF1KB8454
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AccI AccIII AclI AcsI AflII
AhdI Alw44I Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AvrII BalI BamHI BbrPI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BsrDI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DraI DrdI Eam1105I EarI Eco47III
EcoO65I EcoRI EcoRV EcoT22I FbaI
HindIII HpaI Ksp632I MaeI MamI
MfeI MluI MluNI MroI MscI
MseI MunI NcoI NdeI NheI
NruI NsiI NspV PacI PaeR7I
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SapI SbfI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
Tsp509I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AatII 931 GACGTC GACGT^C
Acc65I 449 GGTACC G^GTACC
AflIII 1439 ACATGT A^C[GA][CT]GT
AgeI 1475 ACCGGT A^CCGGT
AlwNI 244 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 409 CCTGAGG CC^T[ACGT]AGG
Asp718I 449 GGTACC G^GTACC
BbvCI 120 CCTCAGC CCTCAGC(-5/-2)
BsaWI 1475 ACCGGT [AT]^CCGG[AT]
BspLU11I 1439 ACATGT A^CATGT
BstAPI 243 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 365 CCAAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 959 AGATCC [GA]^GATC[CT]
Bsu36I 409 CCTGAGG CC^T[ACGT]AGG
Eco81I 409 CCTGAGG CC^T[ACGT]AGG
EcoNI 885 CCTCACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI 42 GGCCGGCC GGCCGG^CC
HincII 640 GTCAAC GT[CT]^[GA]AC
HindII 640 GTCAAC GT[CT]^[GA]AC
HinfI 715 GAGTC G^A[ACGT]TC
KpnI 449 GGTACC GGTAC^C
MflI 959 AGATCC [GA]^GATC[CT]
NotI 744 GCGGCCGC GC^GGCCGC
PinAI 1475 ACCGGT A^CCGGT
PleI 715 GAGTC GAGTC(4/5)
PvuII 540 CAGCTG CAG^CTG
SacI 274 GAGCTC GAGCT^C
SexAI 131 ACCTGGT A^CC[AT]GGT
XcmI 584 CCAAGGAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 959 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BbeI 487 GGCGCC GGCGC^C
1538 GGCGCC
BbsI 814 GTCTTC GAAGAC(2/6)
934 GTCTTC
BglI 518 GCCTGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
522 GCCCGGCTGGC
BpmI 373 CTGGAG CTGGAG(16/14)
1075 CTGGAG
BpuAI 814 GTCTTC GAAGAC(2/6)
934 GTCTTC
BsmAI 9 GAGAC GTCTC(1/5)
901 GTCTC
BsmBI 900 CGTCTC CGTCTC(1/5)
9 GAGACG
BsrBI 748 CCGCTC CCGCTC(-3/-3)
589 GAGCGG
DraIII 280 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
799 CACAAGGTG
DsaI 742 CCGCGG C^C[GA][CT]GG
1365 CCGCGG
EcoT14I 332 CCAAGG C^C[AT][AT]GG
584 CCAAGG
KasI 487 GGCGCC G^GCGCC
1538 GGCGCC
KspI 742 CCGCGG CCGC^GG
1365 CCGCGG
NarI 487 GGCGCC GG^CGCC
1538 GGCGCC
PpuMI 1349 GGGACCT [GA]G^G[AT]CC[CT]
1548 GGGTCCC
SacII 742 CCGCGG CCGC^GG
1365 CCGCGG
ScaI 566 AGTACT AGT^ACT
1154 AGTACT
SfoI 487 GGCGCC GGC^GCC
1538 GGCGCC
SmlI 751 CTCAAG C^T[CT][GA]AG
988 CTCAAG
StyI 332 CCAAGG C^C[AT][AT]GG
584 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AspI AvaI AvaII
AviII BanI BanII BbvI BcnI
BmyI BsaHI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BssSI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EagI EclXI
Eco52I EcoO109I EcoRII FauI Fnu4HI
FokI FspI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
HphI ItaI MaeII MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PflFI PflMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
TaqI TseI Tsp45I TspRI Tth111I
TthHB8I Van91I XmaCI XmaI