Restriction map for
pF1KB8457
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# NO CUT ENZYMES ( 130 enzymes) #
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AatII Acc65I AccI AccIII AclI
AfaI AflII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BamHI BbrPI BcgI
BciVI BfrI BglI BglII BlnI
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrDI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstYI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV FseI FspI HgaI
HincII HindII HindIII HpaI KpnI
KspI MamI MfeI MflI MluI
MroI MseI MunI NaeI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I Tsp45I
Van91I XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccII 1007 CGCG CG^CG
AcsI 1135 GAATTT [GA]^AATT[CT]
AflIII 179 ACACGT A^C[GA][CT]GT
ApoI 1135 GAATTT [GA]^AATT[CT]
AspHI 216 GAGCAC G[AT]GC[AT]^C
AspI 851 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 245 TGGCCA TGG^CCA
BbsI 169 GTCTTC GAAGAC(2/6)
BclI 110 TGATCA T^GATCA
BfaI 631 CTAG C^TAG
BlpI 411 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 411 GCTCAGC GC^T[ACGT]AGC
BpuAI 169 GTCTTC GAAGAC(2/6)
BsaI 524 GAGACC GGTCTC(1/5)
BsiHKAI 216 GAGCAC G[AT]GC[AT]^C
BspHI 638 TCATGA T^CATGA
BsrFI 327 ACCGGC [GA]^CCGG[CT]
BssSI 397 CTCGTG CACGAG(-5/-1)
BstAPI 258 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1007 CGCG CG^CG
CelII 411 GCTCAGC GC^T[ACGT]AGC
Cfr10I 327 ACCGGC [GA]^CCGG[CT]
DpnII 111 GATC ^GATC
EarI 1066 GAAGAG CTCTTC(1/4)
EcoT22I 145 ATGCAT ATGCA^T
FbaI 110 TGATCA T^GATCA
Ksp632I 1066 GAAGAG CTCTTC(1/4)
MaeI 631 CTAG C^TAG
MaeII 181 ACGT A^CGT
MaeIII 868 GTAAC ^GT[ACGT]AC
MboI 111 GATC ^GATC
MluNI 245 TGGCCA TGG^CCA
MscI 245 TGGCCA TGG^CCA
MslI 144 CATGCATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 1007 CGCG CG^CG
NcoI 846 CCATGG C^CATGG
NdeII 111 GATC ^GATC
NsiI 145 ATGCAT ATGCA^T
NspI 147 GCATGT [GA]CATG^[CT]
PflFI 851 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 638 TCATGA T^CATGA
Sau3AI 111 GATC ^GATC
Tsp509I 1136 AATT ^AATT
Tth111I 851 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
ApaI 607 GGGCCC GGGCC^C
882 GGGCCC
BpmI 453 CTCCAG CTGGAG(16/14)
671 CTCCAG
BseRI 616 CTCCTC GAGGAG(10/8)
1150 GAGGAG
BsgI 308 CTGCAC GTGCAG(16/14)
480 CTGCAC
BsmAI 524 GAGAC GTCTC(1/5)
667 GAGAC
BspMI 783 ACCTGC ACCTGC(4/8)
933 ACCTGC
EcoT14I 603 CCTTGG C^C[AT][AT]GG
846 CCATGG
HphI 897 GGTGA GGTGA(8/7)
229 TCACC
MboII 1066 GAAGA GAAGA(8/7)
170 TCTTC
SmaI 553 CCCGGG CCC^GGG
1040 CCCGGG
StyI 603 CCTTGG C^C[AT][AT]GG
846 CCATGG
TaqI 419 TCGA T^CGA
599 TCGA
TthHB8I 419 TCGA T^CGA
599 TCGA
XcmI 32 CCACCATGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
455 CCAGCTACGGCCTGG
XmaCI 553 CCCGGG C^CCGGG
1040 CCCGGG
XmaI 553 CCCGGG C^CCGGG
1040 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AcyI AluI AlwNI AvaI
AvaII BanI BanII BbeI BbvCI
BbvI BcnI BmyI BsaHI BsaJI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrBI BsrI BssKI BstF5I BstNI
BstXI Cac8I CfoI Cfr13I DdeI
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII ItaI KasI MnlI MspA1I
MspI MvaI MwoI NarI NciI
NlaIII NlaIV PleI PpuMI PstI
PvuII Sau96I ScrFI SfaNI SfcI
SfoI TseI TspRI