Restriction map for
pF1KB8459
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII AccI AccIII AcyI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I AspEI AspHI
AspI AvaI AviII AvrII BamHI
BbeI BbvCI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DdeI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoRV EcoT22I FbaI
FseI FspI HapII Hin1I HpaI
HpaII KasI KspI MluI MroI
MslI MspI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PpuMI
PshAI PshBI PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 195 GGTACC G^GTACC
AccII 1250 CGCG CG^CG
Asp718I 195 GGTACC G^GTACC
AvaII 777 GGACC G^G[AT]CC
BanI 195 GGTACC G^G[CT][GA]CC
BanII 846 GGGCTC G[GA]GC[CT]^C
BbrPI 520 CACGTG CAC^GTG
BbsI 1167 GAAGAC GAAGAC(2/6)
BcgI 1305 CGAGAAGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 790 CTAG C^TAG
BpuAI 1167 GAAGAC GAAGAC(2/6)
BsaAI 520 CACGTG [CT]AC^GT[GA]
BsaBI 146 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BssKI 889 CCTGG ^CC[ACGT]GG
BstNI 889 CCTGG CC^[AT]GG
BstUI 1250 CGCG CG^CG
BstYI 321 AGATCC [GA]^GATC[CT]
Cac8I 1185 GCGGGC GC[ACGT]^[ACGT]GC
DsaI 83 CCATGG C^C[GA][CT]GG
EcoRI 618 GAATTC G^AATTC
EcoRII 889 CCTGG ^CC[AT]GG
HaeII 235 GGCGCT [GA]GCGC^[CT]
HgaI 212 GACGC GACGC(5/10)
HincII 699 GTCAAC GT[CT]^[GA]AC
HindII 699 GTCAAC GT[CT]^[GA]AC
HindIII 446 AAGCTT A^AGCTT
KpnI 195 GGTACC GGTAC^C
MaeI 790 CTAG C^TAG
MamI 146 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1298 CAATTG C^AATTG
MflI 321 AGATCC [GA]^GATC[CT]
MunI 1298 CAATTG C^AATTG
MvaI 889 CCTGG CC^[AT]GG
MvnI 1250 CGCG CG^CG
NcoI 83 CCATGG C^CATGG
PleI 697 GAGTC GAGTC(4/5)
PmaCI 520 CACGTG CAC^GTG
PmlI 520 CACGTG CAC^GTG
PvuII 647 CAGCTG CAG^CTG
ScrFI 889 CCTGG CC^[ACGT]GG
SfcI 1154 CTACAG C^T[GA][CT]AG
SspI 575 AATATT AAT^ATT
TaqI 265 TCGA T^CGA
TthHB8I 265 TCGA T^CGA
XcmI 953 CCACTAAACCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 321 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 618 GAATTC [GA]^AATT[CT]
1379 AAATTT
AlwI 322 GATCC GGATC(4/5)
45 GGATC
ApoI 618 GAATTC [GA]^AATT[CT]
1379 AAATTT
BalI 2 TGGCCA TGG^CCA
86 TGGCCA
BmyI 287 GTGCCC G[AGT]GC[ACT]^C
846 GGGCTC
BsmAI 660 GTCTC GTCTC(1/5)
937 GTCTC
BsmFI 404 GGGAC GGGAC(10/14)
862 GTCCC
BsmI 994 GAATGC GAATGC(1/-1)
1311 GAATGC
Bsp1286I 287 GTGCCC G[AGT]GC[ACT]^C
846 GGGCTC
BstF5I 1359 GGATG GGATG(2/0)
1398 GGATG
CfoI 132 GCGC GCG^C
236 GCGC
EcoNI 199 CCTTCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
250 CCTTTGGAAGG
FauI 501 GCGGG CCCGC(4/6)
1185 GCGGG
FokI 1359 GGATG GGATG(9/13)
1398 GGATG
HhaI 132 GCGC GCG^C
236 GCGC
HinP1I 132 GCGC G^CGC
236 GCGC
MluNI 2 TGGCCA TGG^CCA
86 TGGCCA
MscI 2 TGGCCA TGG^CCA
86 TGGCCA
MspA1I 314 CAGCGG C[AC]G^C[GT]G
647 CAGCTG
Tsp45I 983 GTGAC ^GT[GC]AC
1327 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AclI AfaI AluI BbvI
BpmI BsaJI BseRI BsiYI BslI
BsrI Cfr13I DpnII DraI EaeI
EarI EcoT14I Fnu4HI HaeIII HinfI
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MnlI MseI MwoI
NdeII NlaIII NlaIV Psp1406I RsaI
Sau3AI Sau96I SfaNI StyI TfiI
Tru9I TseI Tsp509I TspRI