Restriction map for
pF1KB8463
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AvrII BamHI BbeI BbsI
BcgI BciVI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsmFI
BspDI BspEI BspHI BspLU11I BsrBI
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV FseI
HincII HindII HinfI HpaI HphI
KasI KpnI Ksp632I KspI MboII
MfeI MflI MluI MroI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PinAI
PleI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI SspI StuI SwaI
TfiI Tsp509I XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AatII 691 GACGTC GACGT^C
AccII 863 CGCG CG^CG
AcyI 691 GACGTC G[GA]^CG[CT]C
AfaI 384 GTAC GT^AC
AlwI 1013 GGATC GGATC(4/5)
AspI 322 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 248 TGCGCA TGC^GCA
BalI 69 TGGCCA TGG^CCA
BbrPI 934 CACGTG CAC^GTG
BbvCI 597 CCTCAGC CCTCAGC(-5/-2)
BclI 779 TGATCA T^GATCA
BsaAI 934 CACGTG [CT]AC^GT[GA]
BsaBI 1014 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 691 GACGTC G[GA]^CG[CT]C
BsgI 841 CTGCAC GTGCAG(16/14)
BsmAI 964 GAGAC GTCTC(1/5)
BsmI 870 GAATGC GAATGC(1/-1)
Bsp1407I 383 TGTACA T^GTACA
BspMI 474 GCAGGT ACCTGC(4/8)
BsrDI 566 GCAATG GCAATG(2/0)
BsrFI 754 GCCGGT [GA]^CCGG[CT]
BsrGI 383 TGTACA T^GTACA
BssHII 862 GCGCGC G^CGCGC
BstUI 863 CGCG CG^CG
BstXI 953 CCATGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 754 GCCGGT [GA]^CCGG[CT]
DdeI 598 CTCAG C^T[ACGT]AG
DrdI 517 GACACACTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 953 CCATGG C^C[GA][CT]GG
EcoT22I 160 ATGCAT ATGCA^T
FauI 1002 GCGGG CCCGC(4/6)
FbaI 779 TGATCA T^GATCA
FspI 248 TGCGCA TGC^GCA
HaeII 601 AGCGCC [GA]GCGC^[CT]
HgaI 930 GACGC GACGC(5/10)
Hin1I 691 GACGTC G[GA]^CG[CT]C
HindIII 218 AAGCTT A^AGCTT
MaeIII 152 GTGAC ^GT[ACGT]AC
MamI 1014 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 69 TGGCCA TGG^CCA
MscI 69 TGGCCA TGG^CCA
MvnI 863 CGCG CG^CG
NcoI 953 CCATGG C^CATGG
NsiI 160 ATGCAT ATGCA^T
PaeR7I 659 CTCGAG C^TCGAG
PflFI 322 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 288 CCATAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 934 CACGTG CAC^GTG
PmlI 934 CACGTG CAC^GTG
PpuMI 113 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 400 CAGCTG CAG^CTG
RsaI 384 GTAC GT^AC
SbfI 534 CCTGCAGG CCTGCA^GG
SfaNI 544 GATGC GCATC(5/9)
Sse8387I 534 CCTGCAGG CCTGCA^GG
SspBI 383 TGTACA T^GTACA
Tsp45I 152 GTGAC ^GT[GC]AC
Tth111I 322 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 288 CCATAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 312 TCTAGA T^CTAGA
XcmI 548 CCACCGAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 659 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
ApaI 77 GGGCCC GGGCC^C
422 GGGCCC
AspHI 268 GTGCTC G[AT]GC[AT]^C
316 GAGCAC
BanI 411 GGTGCC G^G[CT][GA]CC
757 GGTGCC
BanII 77 GGGCCC G[GA]GC[CT]^C
422 GGGCCC
BcnI 372 CCGGG CC^[GC]GG
1010 CCCGG
BfaI 313 CTAG C^TAG
416 CTAG
BsiHKAI 268 GTGCTC G[AT]GC[AT]^C
316 GAGCAC
EaeI 69 TGGCCA [CT]^GGCC[GA]
986 TGGCCG
EcoNI 381 CCTGTACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
534 CCTGCAGGAGG
MaeI 313 CTAG C^TAG
416 CTAG
MaeII 692 ACGT A^CGT
935 ACGT
MseI 35 TTAA T^TAA
644 TTAA
MspA1I 400 CAGCTG C[AC]G^C[GT]G
1000 CAGCGG
NciI 372 CCGGG CC^[GC]GG
1010 CCCGG
NspI 395 ACATGC [GA]CATG^[CT]
735 GCATGT
SmlI 659 CTCGAG C^T[CT][GA]AG
980 CTCAAG
TaqI 660 TCGA T^CGA
1031 TCGA
Tru9I 35 TTAA T^TAA
644 TTAA
TthHB8I 660 TCGA T^CGA
1031 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AluI AvaI AvaII BbvI
BglI BmyI BpmI BsaJI BseRI
BsiYI BslI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DpnII DraII EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII ItaI MboI
MnlI MslI MspI MvaI MwoI
NdeII NlaIII NlaIV PstI Sau3AI
Sau96I ScrFI SfcI StyI TseI
TspRI