Restriction map for
pF1KB8464
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# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AvrII
BbrPI BbvCI BciVI BclI BfrI
BlnI BsaAI BsaWI BseAI BsiHKAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrGI BssHII BssSI
Bst1107I BstBI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco81I EcoRI EcoRV EcoT22I FbaI
FseI HgaI HindIII HpaI KpnI
KspI MfeI MluI MroI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI ScaI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SspBI SwaI Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccII 1866 CGCG CG^CG
AcyI 533 GGCGCC G[GA]^CG[CT]C
Aor51HI 935 AGCGCT AGC^GCT
ApaI 914 GGGCCC GGGCC^C
AvaI 707 CCCGAG C^[CT]CG[GA]G
BamHI 209 GGATCC G^GATCC
BbeI 533 GGCGCC GGCGC^C
BcgI 1320 GCAGCGGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1511 GCCACTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 650 AGATCT A^GATCT
BlpI 1408 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1408 GCTGAGC GC^T[ACGT]AGC
BsaHI 533 GGCGCC G[GA]^CG[CT]C
BsiEI 5 CGGCCG CG[GA][CT]^CG
BsoBI 707 CCCGAG C^[CT]CG[GA]G
BspHI 2232 TCATGA T^CATGA
BspMI 2172 GCAGGT ACCTGC(4/8)
BsrDI 456 CATTGC GCAATG(2/0)
BsrFI 3 GCCGGC [GA]^CCGG[CT]
BstEII 1497 GGTGACC G^GT[ACGT]ACC
BstPI 1497 GGTGACC G^GT[ACGT]ACC
BstUI 1866 CGCG CG^CG
CelII 1408 GCTGAGC GC^T[ACGT]AGC
Cfr10I 3 GCCGGC [GA]^CCGG[CT]
DraII 913 GGGGCCC [GA]G^G[ACGT]CC[CT]
EagI 5 CGGCCG C^GGCCG
EclXI 5 CGGCCG C^GGCCG
Eco47III 935 AGCGCT AGC^GCT
Eco52I 5 CGGCCG C^GGCCG
EcoNI 924 CCTCATTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 913 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 1497 GGTGACC G^GT[ACGT]ACC
Hin1I 533 GGCGCC G[GA]^CG[CT]C
HincII 157 GTTGAC GT[CT]^[GA]AC
HindII 157 GTTGAC GT[CT]^[GA]AC
KasI 533 GGCGCC G^GCGCC
MseI 2219 TTAA T^TAA
MvnI 1866 CGCG CG^CG
NaeI 3 GCCGGC GCC^GGC
NarI 533 GGCGCC GG^CGCC
NcoI 2024 CCATGG C^CATGG
NgoMIV 3 GCCGGC G^CCGGC
PleI 982 GAGTC GAGTC(4/5)
PstI 2252 CTGCAG CTGCA^G
RcaI 2232 TCATGA T^CATGA
SbfI 2251 CCTGCAGG CCTGCA^GG
SfoI 533 GGCGCC GGC^GCC
SphI 2246 GCATGC GCATG^C
Sse8387I 2251 CCTGCAGG CCTGCA^GG
SspI 151 AATATT AAT^ATT
StuI 1599 AGGCCT AGG^CCT
Tru9I 2219 TTAA T^TAA
Tsp45I 1498 GTGAC ^GT[GC]AC
XcmI 1871 CCAGAATCAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 196 GAATTT [GA]^AATT[CT]
751 AAATTC
ApoI 196 GAATTT [GA]^AATT[CT]
751 AAATTC
AspI 1153 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2203 GACCAGGTC
AviII 1286 TGCGCA TGC^GCA
2093 TGCGCA
BbsI 2055 GAAGAC GAAGAC(2/6)
2182 GTCTTC
BcnI 1529 CCCGG CC^[GC]GG
1793 CCCGG
BfaI 206 CTAG C^TAG
631 CTAG
BpuAI 2055 GAAGAC GAAGAC(2/6)
2182 GTCTTC
BsaBI 1174 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1222 GATGAGTATC
BsaI 415 GAGACC GGTCTC(1/5)
2208 GGTCTC
BsgI 98 CTGCAC GTGCAG(16/14)
16 GTGCAG
BsmFI 1236 GTCCC GGGAC(10/14)
1981 GTCCC
BsrBI 799 GAGCGG CCGCTC(-3/-3)
1411 GAGCGG
DsaI 47 CCACGG C^C[GA][CT]GG
2024 CCATGG
EarI 610 CTCTTC CTCTTC(1/4)
781 GAAGAG
EcoT14I 341 CCAAGG C^C[AT][AT]GG
2024 CCATGG
FspI 1286 TGCGCA TGC^GCA
2093 TGCGCA
HaeII 533 GGCGCC [GA]GCGC^[CT]
935 AGCGCT
Ksp632I 610 CTCTTC CTCTTC(1/4)
781 GAAGAG
MaeI 206 CTAG C^TAG
631 CTAG
MaeII 762 ACGT A^CGT
1880 ACGT
MaeIII 731 GTTAC ^GT[ACGT]AC
1498 GTGAC
MamI 1174 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1222 GATGAGTATC
NciI 1529 CCCGG CC^[GC]GG
1793 CCCGG
PflFI 1153 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2203 GACCAGGTC
SexAI 1371 ACCAGGT A^CC[AT]GGT
2204 ACCAGGT
SmlI 685 CTCAAG C^T[CT][GA]AG
2353 CTCAAG
StyI 341 CCAAGG C^C[AT][AT]GG
2024 CCATGG
Tsp509I 197 AATT ^AATT
752 AATT
Tth111I 1153 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2203 GACCAGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BalI BanI BanII BbvI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EaeI EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TfiI TseI TspRI TthHB8I
XhoII