##################################
# NO CUT ENZYMES (  198 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AcyI        AflII       AhdI        Alw44I      
AlwI        AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BcnI        
BfaI        BfrI        BglII       BlnI        BlpI        
BmyI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsiYI       BslI        
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BsrBI       BsrDI       BsrGI       
BsrI        BssHII      BssKI       BssSI       BstAPI      
BstBI       BstEII      BstNI       BstPI       BstUI       
BstXI       BstYI       Bsu36I      CelII       CfoI        
ClaI        CpoI        DpnII       DraI        DraII       
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRII      EcoRV       EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HinfI       HpaI        KasI        KpnI        
KspI        MaeI        MamI        MboI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MslI        MunI        MvaI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NdeII       
NgoMIV      NheI        NlaIV       NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        Sau3AI      SbfI        ScrFI       
SexAI       SfaNI       SfcI        SfoI        SfuI        
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
TfiI        Tsp509I     TspRI       Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AccI          181  GTATAC               GT^[AC][GT]AC
AfaI           25  GTAC                 GT^AC
AflIII         55  ACATGT               A^C[GA][CT]GT
AgeI           70  ACCGGT               A^CCGGT
AlwNI         278  CAGGGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
BglI          313  GCCCAAGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI          172  CTGGAG               CTGGAG(16/14)
BsaWI          70  ACCGGT               [AT]^CCGG[AT]
BsmAI          61  GAGAC                GTCTC(1/5)
BsmBI          61  GAGACG               CGTCTC(1/5)
BsmFI         348  GGGAC                GGGAC(10/14)
BspLU11I       55  ACATGT               A^CATGT
BspMI         177  GCAGGT               ACCTGC(4/8)
BsrFI          70  ACCGGT               [GA]^CCGG[CT]
Bst1107I      181  GTATAC               GTA^TAC
BstF5I        186  CATCC                GGATG(2/0)
BstZ17I       181  GTATAC               GTA^TAC
Cac8I         283  GCTGGC               GC[ACGT]^[ACGT]GC
Cfr10I         70  ACCGGT               [GA]^CCGG[CT]
DdeI          276  CTCAG                C^T[ACGT]AG
DraIII         46  CACATTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          189  CCGTGG               C^C[GA][CT]GG
EarI          332  GAAGAG               CTCTTC(1/4)
EcoT14I       270  CCAAGG               C^C[AT][AT]GG
FokI          186  CATCC                GGATG(9/13)
HapII          71  CCGG                 C^CGG
HpaII          71  CCGG                 C^CGG
HphI           73  GGTGA                GGTGA(8/7)
Ksp632I       332  GAAGAG               CTCTTC(1/4)
MspA1I        169  CAGCTG               C[AC]G^C[GT]G
MspI           71  CCGG                 C^CGG
PinAI          70  ACCGGT               A^CCGGT
PvuII         169  CAGCTG               CAG^CTG
RsaI           25  GTAC                 GT^AC
ScaI           24  AGTACT               AGT^ACT
SfiI          312  GGCCCAAGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI          69  CACCGGTG             C[GA]^CCGG[CT]G
SmlI          307  CTCAAG               C^T[CT][GA]AG
StyI          270  CCAAGG               C^C[AT][AT]GG


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

AciI          204  CCGC                 CCGC(-3/-1)
              323  CCGC                
BbvI          194  GCAGC                GCAGC(8/12)
              298  GCTGC               
Cfr13I         42  GGGCC                G^G[ACGT]CC
              312  GGCCC               
MaeII         339  ACGT                 A^CGT
              351  ACGT                
MaeIII         52  GTGAC                ^GT[ACGT]AC
              142  GTCAC               
MseI           20  TTAA                 T^TAA
              247  TTAA                
NspI           55  ACATGT               [GA]CATG^[CT]
              224  ACATGC              
Sau96I         42  GGGCC                G^G[ACGT]CC
              312  GGCCC               
Tru9I          20  TTAA                 T^TAA
              247  TTAA                
TseI          194  GCAGC                G^C[AT]GC
              298  GCTGC               
Tsp45I         52  GTGAC                ^GT[GC]AC
              142  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   10 enzymes) #
#########################################

AluI        BsaJI       EaeI        Fnu4HI      HaeIII      
ItaI        MboII       MnlI        MwoI        NlaIII