Restriction map for
pF1KB8467
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BbvCI BcgI BciVI BfrI BlnI
BsaBI BsaHI BsaWI BseAI BseRI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoRI EcoRV
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MseI MunI NaeI NarI NcoI
NdeI NgoMIV NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PpuMI PshBI Psp1406I PvuI
RsrII SacII SalI ScaI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SspBI SspI SwaI Tru9I Tth111I
Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 1030 GTAGAC GT^[AC][GT]AC
AccII 816 CGCG CG^CG
AlwNI 876 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 64 GAGCTC G[AT]GC[AT]^C
BalI 1103 TGGCCA TGG^CCA
BbrPI 22 CACGTG CAC^GTG
BbsI 1267 GAAGAC GAAGAC(2/6)
BclI 275 TGATCA T^GATCA
BfaI 119 CTAG C^TAG
BglII 204 AGATCT A^GATCT
BlpI 1131 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1131 GCTCAGC GC^T[ACGT]AGC
BpuAI 1267 GAAGAC GAAGAC(2/6)
BsaAI 22 CACGTG [CT]AC^GT[GA]
BsgI 1111 GTGCAG GTGCAG(16/14)
BsiEI 1079 CGACCG CG[GA][CT]^CG
BsiHKAI 64 GAGCTC G[AT]GC[AT]^C
BsmI 7 GAATGC GAATGC(1/-1)
BspHI 197 TCATGA T^CATGA
BstAPI 50 GCAATGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 807 GGTCACC G^GT[ACGT]ACC
BstPI 807 GGTCACC G^GT[ACGT]ACC
BstUI 816 CGCG CG^CG
BstXI 414 CCACAGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 204 AGATCT [GA]^GATC[CT]
CelII 1131 GCTCAGC GC^T[ACGT]AGC
EcoO65I 807 GGTCACC G^GT[ACGT]ACC
EcoT22I 9 ATGCAT ATGCA^T
FbaI 275 TGATCA T^GATCA
HaeII 659 AGCGCC [GA]GCGC^[CT]
MaeI 119 CTAG C^TAG
MaeIII 808 GTCAC ^GT[ACGT]AC
MflI 204 AGATCT [GA]^GATC[CT]
MluNI 1103 TGGCCA TGG^CCA
MscI 1103 TGGCCA TGG^CCA
MslI 1216 CATTGGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 816 CGCG CG^CG
NheI 118 GCTAGC G^CTAGC
NsiI 9 ATGCAT ATGCA^T
PleI 139 GACTC GAGTC(4/5)
PmaCI 22 CACGTG CAC^GTG
PmlI 22 CACGTG CAC^GTG
PvuII 271 CAGCTG CAG^CTG
RcaI 197 TCATGA T^CATGA
SacI 64 GAGCTC GAGCT^C
SbfI 393 CCTGCAGG CCTGCA^GG
SmlI 113 CTCAAG C^T[CT][GA]AG
Sse8387I 393 CCTGCAGG CCTGCA^GG
StuI 1157 AGGCCT AGG^CCT
TfiI 1312 GATTC G^A[AT]TC
Tsp45I 808 GTCAC ^GT[GC]AC
XhoII 204 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 1249 GAATTT [GA]^AATT[CT]
1342 GAATTT
AfaI 933 GTAC GT^AC
1194 GTAC
ApaI 737 GGGCCC GGGCC^C
1163 GGGCCC
ApoI 1249 GAATTT [GA]^AATT[CT]
1342 GAATTT
BanI 777 GGTGCC G^G[CT][GA]CC
969 GGTGCC
BglI 392 GCCTGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1198 GCCAAGCGGGC
BsaI 1097 GAGACC GGTCTC(1/5)
528 GGTCTC
CfoI 660 GCGC GCG^C
817 GCGC
DraII 736 GGGGCCC [GA]G^G[ACGT]CC[CT]
737 GGGCCCT
DsaI 1082 CCGTGG C^C[GA][CT]GG
1142 CCACGG
EarI 67 CTCTTC CTCTTC(1/4)
256 CTCTTC
EcoNI 1101 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1281 CCTGGAGAAGG
EcoO109I 736 GGGGCCC [GA]G^G[ACGT]CC[CT]
737 GGGCCCT
EcoT14I 974 CCAAGG C^C[AT][AT]GG
1106 CCAAGG
HhaI 660 GCGC GCG^C
817 GCGC
HinP1I 660 GCGC G^CGC
817 GCGC
HinfI 139 GACTC G^A[ACGT]TC
1312 GATTC
Ksp632I 67 CTCTTC CTCTTC(1/4)
256 CTCTTC
MaeII 23 ACGT A^CGT
965 ACGT
PshAI 1033 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1080 GACCGTGGTC
PstI 394 CTGCAG CTGCA^G
536 CTGCAG
RsaI 933 GTAC GT^AC
1194 GTAC
SapI 66 GCTCTTC GCTCTTC(1/4)
255 GCTCTTC
SexAI 347 ACCTGGT A^CC[AT]GGT
773 ACCTGGT
SphI 947 GCATGC GCATG^C
1127 GCATGC
StyI 974 CCAAGG C^C[AT][AT]GG
1106 CCAAGG
TaqI 1172 TCGA T^CGA
1184 TCGA
Tsp509I 1250 AATT ^AATT
1343 AATT
TthHB8I 1172 TCGA T^CGA
1184 TCGA
XcmI 414 CCACAGTTTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
893 CCAACAGCCAGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AluI AvaI AvaII BanII
BbvI BcnI BmyI BpmI BsaJI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EaeI EcoRII FauI Fnu4HI FokI
HaeIII HapII HpaII HphI ItaI
MboI MboII MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI TseI TspRI
XmaCI XmaI