Restriction map for
pF1KB8566
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# NO CUT ENZYMES ( 165 enzymes) #
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AatII Acc65I AccI AccIII AcyI
AflII AgeI AhdI Alw44I AlwI
AlwNI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BfrI
BglI BlnI BlpI BmyI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I CelII CfoI Cfr10I
Cfr13I ClaI CpoI DraI DraII
DraIII DrdI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoNI
EcoO109I EcoO65I EcoRI EcoT14I EcoT22I
FseI FspI HaeII HaeIII HgaI
HhaI Hin1I HinP1I HindIII KasI
KpnI Ksp632I MamI MfeI MluI
MluNI MroI MscI MslI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NlaIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PvuI RcaI
RsrII SacI SalI SapI Sau96I
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI StyI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccII 6 CGCG CG^CG
AclI 723 AACGTT AA^CGTT
AcsI 37 AAATTC [GA]^AATT[CT]
AflIII 504 ACGTGT A^C[GA][CT]GT
AocI 586 CCTCAGG CC^T[ACGT]AGG
ApoI 37 AAATTC [GA]^AATT[CT]
AseI 717 ATTAAT AT^TAAT
AsnI 717 ATTAAT AT^TAAT
Asp700I 702 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI 556 GAAGAC GAAGAC(2/6)
BbvCI 30 GCTGAGG CCTCAGC(-5/-2)
BcgI 492 GCAGCTCTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 86 TGATCA T^GATCA
BcnI 523 CCGGG CC^[GC]GG
BglII 384 AGATCT A^GATCT
BpuAI 556 GAAGAC GAAGAC(2/6)
BseRI 415 GAGGAG GAGGAG(10/8)
BsiYI 92 CCAGATTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 92 CCAGATTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 220 CGTCTC CGTCTC(1/5)
BsrDI 740 CATTGC GCAATG(2/0)
BssSI 753 CTCGTG CACGAG(-5/-1)
BstUI 6 CGCG CG^CG
BstYI 384 AGATCT [GA]^GATC[CT]
Bsu36I 586 CCTCAGG CC^T[ACGT]AGG
Cac8I 7 GCGGGC GC[ACGT]^[ACGT]GC
DsaI 5 CCGCGG C^C[GA][CT]GG
Eco81I 586 CCTCAGG CC^T[ACGT]AGG
EcoRV 280 GATATC GAT^ATC
FbaI 86 TGATCA T^GATCA
HapII 523 CCGG C^CGG
HincII 292 GTTAAC GT[CT]^[GA]AC
HindII 292 GTTAAC GT[CT]^[GA]AC
HpaI 292 GTTAAC GTT^AAC
HpaII 523 CCGG C^CGG
HphI 89 TCACC GGTGA(8/7)
KspI 5 CCGCGG CCGC^GG
MflI 384 AGATCT [GA]^GATC[CT]
MspI 523 CCGG C^CGG
MvnI 6 CGCG CG^CG
MwoI 585 GCCTCAGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 523 CCGGG CC^[GC]GG
PshBI 717 ATTAAT AT^TAAT
Psp1406I 723 AACGTT AA^CGTT
PvuII 263 CAGCTG CAG^CTG
SacII 5 CCGCGG CCGC^GG
SspI 691 AATATT AAT^ATT
TaqI 501 TCGA T^CGA
Tsp45I 566 GTGAC ^GT[GC]AC
TspRI 684 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 501 TCGA T^CGA
XhoII 384 AGATCT [GA]^GATC[CT]
XmnI 702 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AfaI 165 GTAC GT^AC
243 GTAC
BciVI 201 GGATAC GTATCC(6/5)
478 GGATAC
BfaI 367 CTAG C^TAG
464 CTAG
BsrI 475 ACTGG ACTGG(1/-1)
576 ACTGG
BstF5I 246 CATCC GGATG(2/0)
174 GGATG
BstNI 54 CCTGG CC^[AT]GG
239 CCAGG
DpnII 87 GATC ^GATC
385 GATC
EcoRII 54 CCTGG ^CC[AT]GG
239 CCAGG
FauI 7 GCGGG CCCGC(4/6)
208 GCGGG
FokI 246 CATCC GGATG(9/13)
174 GGATG
MaeI 367 CTAG C^TAG
464 CTAG
MaeIII 448 GTTAC ^GT[ACGT]AC
566 GTGAC
MboI 87 GATC ^GATC
385 GATC
MboII 556 GAAGA GAAGA(8/7)
640 TCTTC
MseI 293 TTAA T^TAA
718 TTAA
MvaI 54 CCTGG CC^[AT]GG
239 CCAGG
NdeII 87 GATC ^GATC
385 GATC
NlaIII 561 CATG CATG^
633 CATG
PstI 260 CTGCAG CTGCA^G
681 CTGCAG
RsaI 165 GTAC GT^AC
243 GTAC
Sau3AI 87 GATC ^GATC
385 GATC
Tru9I 293 TTAA T^TAA
718 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AciI AluI BbvI BsaJI BsmAI
BssKI DdeI Fnu4HI HinfI ItaI
MaeII MnlI MspA1I ScrFI SfcI
TfiI TseI Tsp509I