Restriction map for
pF1KB8568
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstBI BstEII
BstPI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I ClaI CpoI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I FauI FseI FspI
HaeII HaeIII HhaI Hin1I HinP1I
HpaI KasI KpnI KspI MamI
MfeI MflI MluNI MroI MscI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SphI
Sse8387I SspBI StuI SwaI TaqI
Tth111I TthHB8I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 281 GTATAC GT^[AC][GT]AC
AccII 1313 CGCG CG^CG
AciI 1738 GCGG CCGC(-3/-1)
AflIII 1312 ACGCGT A^C[GA][CT]GT
AlwI 1129 GATCC GGATC(4/5)
AseI 2170 ATTAAT AT^TAAT
AsnI 2170 ATTAAT AT^TAAT
Asp700I 1351 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1701 GAGCAC G[AT]GC[AT]^C
AvaII 2123 GGACC G^G[AT]CC
BclI 171 TGATCA T^GATCA
BcnI 307 CCCGG CC^[GC]GG
BmyI 1701 GAGCAC G[AGT]GC[ACT]^C
BsaAI 2203 TACGTA [CT]AC^GT[GA]
BsiHKAI 1701 GAGCAC G[AT]GC[AT]^C
Bsp1286I 1701 GAGCAC G[AGT]GC[ACT]^C
Bst1107I 281 GTATAC GTA^TAC
BstAPI 260 GCAATACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1313 CGCG CG^CG
BstZ17I 281 GTATAC GTA^TAC
Cfr13I 2123 GGACC G^G[ACGT]CC
DraII 2122 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO109I 2122 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 2292 GAATTC G^AATTC
EcoRV 581 GATATC GAT^ATC
EcoT22I 1747 ATGCAT ATGCA^T
FbaI 171 TGATCA T^GATCA
HapII 308 CCGG C^CGG
HgaI 1311 GACGC GACGC(5/10)
HincII 1843 GTTGAC GT[CT]^[GA]AC
HindII 1843 GTTGAC GT[CT]^[GA]AC
HpaII 308 CCGG C^CGG
MaeII 2204 ACGT A^CGT
MluI 1312 ACGCGT A^CGCGT
MspA1I 1297 CAGCTG C[AC]G^C[GT]G
MspI 308 CCGG C^CGG
MvnI 1313 CGCG CG^CG
NciI 307 CCCGG CC^[GC]GG
NsiI 1747 ATGCAT ATGCA^T
NspI 640 ACATGC [GA]CATG^[CT]
PflMI 870 CCAGTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2122 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 2170 ATTAAT AT^TAAT
PstI 185 CTGCAG CTGCA^G
PvuII 1297 CAGCTG CAG^CTG
Sau96I 2123 GGACC G^G[ACGT]CC
SmlI 125 CTCAAG C^T[CT][GA]AG
SnaBI 2203 TACGTA TAC^GTA
SpeI 2107 ACTAGT A^CTAGT
Van91I 870 CCAGTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1351 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 1378 GTAC GT^AC
2075 GTAC
BbvI 187 GCAGC GCAGC(8/12)
1606 GCTGC
BseRI 1405 GAGGAG GAGGAG(10/8)
1885 GAGGAG
BsmAI 891 GAGAC GTCTC(1/5)
2104 GAGAC
BsmI 765 GCATTC GAATGC(1/-1)
1049 GCATTC
BstF5I 712 CATCC GGATG(2/0)
372 GGATG
DraI 313 TTTAAA TTT^AAA
778 TTTAAA
Fnu4HI 187 GCAGC GC^[ACGT]GC
1606 GCTGC
FokI 712 CATCC GGATG(9/13)
372 GGATG
HindIII 143 AAGCTT A^AGCTT
2214 AAGCTT
HphI 1249 GGTGA GGTGA(8/7)
199 TCACC
ItaI 187 GCAGC GC^[ACGT]GC
1606 GCTGC
MslI 744 CATAGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1365 CATCAGTGTG
MwoI 260 GCAATACTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
2071 GCTAGTACAGC
NlaIV 2122 GGGACC GG[ACGT]^[ACGT]CC
2123 GGACCC
RsaI 1378 GTAC GT^AC
2075 GTAC
SfcI 185 CTGCAG C^T[GA][CT]AG
586 CTACAG
TseI 187 GCAGC G^C[AT]GC
1606 GCTGC
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# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AcsI AluI ApoI BbsI BfaI
BpmI BpuAI BsaJI BsiYI BslI
BsmFI BsrI BssKI BstNI DdeI
DpnII EarI EcoRII EcoT14I HinfI
Ksp632I MaeI MaeIII MboI MboII
MnlI MseI MvaI NdeII NlaIII
PleI Sau3AI ScrFI SfaNI SspI
StyI TfiI Tru9I Tsp45I Tsp509I
TspRI