Restriction map for
pF1KB8570
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# NO CUT ENZYMES ( 103 enzymes) #
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AatII Acc65I AclI AgeI AhdI
AlwNI AocI ApaI AscI AseI
AsnI Asp718I AspEI AvaII AviII
AvrII BamHI BbrPI BcgI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsgI BsiEI BsiWI BspDI
BspMI BsrFI BstBI BstEII BstPI
BstXI Bsu36I CelII Cfr10I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoT22I FauI FseI FspI
HindIII KpnI MamI MfeI MluI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacI SalI SbfI
ScaI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
SwaI TaqI TthHB8I Van91I XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccIII 73 TCCGGA T^CCGGA
AflIII 686 ACATGT A^C[GA][CT]GT
Alw44I 467 GTGCAC G^TGCAC
Aor51HI 149 AGCGCT AGC^GCT
ApaLI 467 GTGCAC G^TGCAC
Asp700I 3 GAAGTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 55 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 904 CCCGAG C^[CT]CG[GA]G
BalI 231 TGGCCA TGG^CCA
BanI 367 GGCGCC G^G[CT][GA]CC
BanII 45 GGGCTC G[GA]GC[CT]^C
BbeI 367 GGCGCC GGCGC^C
BbsI 673 GTCTTC GAAGAC(2/6)
BbvCI 1276 CCTCAGC CCTCAGC(-5/-2)
BclI 2367 TGATCA T^GATCA
BcnI 87 CCGGG CC^[GC]GG
BglI 23 GCCGCAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 673 GTCTTC GAAGAC(2/6)
BsaI 173 GAGACC GGTCTC(1/5)
BseAI 73 TCCGGA T^CCGGA
BseRI 2414 GAGGAG GAGGAG(10/8)
BsmBI 594 GAGACG CGTCTC(1/5)
BsmI 1711 GCATTC GAATGC(1/-1)
BsoBI 904 CCCGAG C^[CT]CG[GA]G
BspEI 73 TCCGGA T^CCGGA
BspHI 1509 TCATGA T^CATGA
BspLU11I 686 ACATGT A^CATGT
BsrBI 168 GAGCGG CCGCTC(-3/-3)
BssHII 160 GCGCGC G^CGCGC
Bst1107I 619 GTATAC GTA^TAC
BstAPI 1555 GCAGATATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 619 GTATAC GTA^TAC
DraI 2373 TTTAAA TTT^AAA
DraII 978 AGGGCCT [GA]G^G[ACGT]CC[CT]
EaeI 231 TGGCCA [CT]^GGCC[GA]
Eco47III 149 AGCGCT AGC^GCT
EcoO109I 978 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRV 388 GATATC GAT^ATC
EcoT14I 462 CCATGG C^C[AT][AT]GG
FbaI 2367 TGATCA T^GATCA
HincII 259 GTTAAC GT[CT]^[GA]AC
HindII 259 GTTAAC GT[CT]^[GA]AC
HpaI 259 GTTAAC GTT^AAC
KasI 367 GGCGCC G^GCGCC
KspI 137 CCGCGG CCGC^GG
MluNI 231 TGGCCA TGG^CCA
MroI 73 TCCGGA T^CCGGA
MscI 231 TGGCCA TGG^CCA
NarI 367 GGCGCC GG^CGCC
NciI 87 CCGGG CC^[GC]GG
NcoI 462 CCATGG C^CATGG
PflFI 55 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 119 CAGCTG CAG^CTG
RcaI 1509 TCATGA T^CATGA
SacII 137 CCGCGG CCGC^GG
SexAI 633 ACCAGGT A^CC[AT]GGT
SfoI 367 GGCGCC GGC^GCC
StyI 462 CCATGG C^C[AT][AT]GG
Tth111I 55 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 3 GAAGTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 61 GTCTAC GT^[AC][GT]AC
619 GTATAC
AccII 138 CGCG CG^CG
161 CGCG
AflII 655 CTTAAG C^TTAAG
1627 CTTAAG
AspHI 432 GAGCAC G[AT]GC[AT]^C
467 GTGCAC
BciVI 76 GGATAC GTATCC(6/5)
1272 GTATCC
BfrI 655 CTTAAG C^TTAAG
1627 CTTAAG
BsaWI 73 TCCGGA [AT]^CCGG[AT]
1715 TCCGGT
BsiHKAI 432 GAGCAC G[AT]GC[AT]^C
467 GTGCAC
BsmFI 89 GGGAC GGGAC(10/14)
901 GTCCC
BsrDI 325 CATTGC GCAATG(2/0)
851 CATTGC
BssSI 1910 CACGAG CACGAG(-5/-1)
2326 CTCGTG
BstUI 138 CGCG CG^CG
161 CGCG
DsaI 137 CCGCGG C^C[GA][CT]GG
462 CCATGG
HaeII 149 AGCGCT [GA]GCGC^[CT]
367 GGCGCC
HgaI 133 GACGC GACGC(5/10)
181 GACGC
HphI 631 TCACC GGTGA(8/7)
2205 TCACC
MslI 1014 CACTGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1504 CATAATCATG
MspA1I 119 CAGCTG C[AC]G^C[GT]G
137 CCGCGG
MvnI 138 CGCG CG^CG
161 CGCG
NspI 576 ACATGC [GA]CATG^[CT]
686 ACATGT
PleI 91 GACTC GAGTC(4/5)
628 GACTC
PstI 100 CTGCAG CTGCA^G
815 CTGCAG
SapI 1108 GAAGAGC GCTCTTC(1/4)
1303 GAAGAGC
SfcI 100 CTGCAG C^T[GA][CT]AG
815 CTGCAG
SspI 664 AATATT AAT^ATT
2441 AATATT
XbaI 1831 TCTAGA T^CTAGA
2455 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI BbvI BfaI BmyI
BsaHI BsaJI BsiYI BslI BsmAI
Bsp1286I Bsp1407I BsrGI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EarI EcoRII
Fnu4HI FokI HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspI
MvaI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI SspBI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII