Restriction map for
pF1KB8571
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# NO CUT ENZYMES ( 134 enzymes) #
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Acc65I AccI AccII AccIII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbeI BbvCI BcgI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaI BsaWI BseAI BsgI
BsiEI BsiWI BspDI BspEI BspMI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstXI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO109I EcoRI EcoRV FauI FseI
FspI HaeII HaeIII HapII HhaI
HinP1I HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MfeI
MflI MluI MluNI MroI MscI
MspI MunI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NspV PaeR7I PflFI PinAI
PmeI PpuMI PshAI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI SwaI Tth111I XbaI XcmI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AatII 1613 GACGTC GACGT^C
AciI 1698 CCGC CCGC(-3/-1)
AcsI 231 AAATTC [GA]^AATT[CT]
AcyI 1613 GACGTC G[GA]^CG[CT]C
ApoI 231 AAATTC [GA]^AATT[CT]
AseI 1206 ATTAAT AT^TAAT
AsnI 1206 ATTAAT AT^TAAT
Asp700I 560 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1219 CCCGAG C^[CT]CG[GA]G
AvaII 169 GGACC G^G[AT]CC
BanI 1280 GGTGCC G^G[CT][GA]CC
BbrPI 149 CACGTG CAC^GTG
BciVI 1579 GGATAC GTATCC(6/5)
BclI 111 TGATCA T^GATCA
BsaAI 149 CACGTG [CT]AC^GT[GA]
BsaBI 59 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1613 GACGTC G[GA]^CG[CT]C
BseRI 1316 CTCCTC GAGGAG(10/8)
BsmI 1455 GAATGC GAATGC(1/-1)
BsoBI 1219 CCCGAG C^[CT]CG[GA]G
Bsp1407I 588 TGTACA T^GTACA
BspHI 980 TCATGA T^CATGA
BspLU11I 1708 ACATGT A^CATGT
BsrBI 1698 CCGCTC CCGCTC(-3/-3)
BsrGI 588 TGTACA T^GTACA
BstEII 1411 GGTTACC G^GT[ACGT]ACC
BstF5I 353 CATCC GGATG(2/0)
BstPI 1411 GGTTACC G^GT[ACGT]ACC
Cfr13I 169 GGACC G^G[ACGT]CC
DraI 52 TTTAAA TTT^AAA
DraIII 1275 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 987 CCATGG C^C[GA][CT]GG
EcoNI 469 CCTAAATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1411 GGTTACC G^GT[ACGT]ACC
EcoT22I 1665 ATGCAT ATGCA^T
FbaI 111 TGATCA T^GATCA
FokI 353 CATCC GGATG(9/13)
Hin1I 1613 GACGTC G[GA]^CG[CT]C
MamI 59 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 987 CCATGG C^CATGG
NsiI 1665 ATGCAT ATGCA^T
NspI 1708 ACATGT [GA]CATG^[CT]
PacI 184 TTAATTAA TTAAT^TAA
PmaCI 149 CACGTG CAC^GTG
PmlI 149 CACGTG CAC^GTG
PshBI 1206 ATTAAT AT^TAAT
PstI 1343 CTGCAG CTGCA^G
RcaI 980 TCATGA T^CATGA
Sau96I 169 GGACC G^G[ACGT]CC
SfcI 1343 CTGCAG C^T[GA][CT]AG
SmlI 1037 CTCAAG C^T[CT][GA]AG
SspBI 588 TGTACA T^GTACA
SspI 563 AATATT AAT^ATT
TaqI 486 TCGA T^CGA
TthHB8I 486 TCGA T^CGA
XmnI 560 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 10 enzymes) #
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AfaI 589 GTAC GT^AC
1657 GTAC
AflIII 916 ACGTGT A^C[GA][CT]GT
1708 ACATGT
BsmBI 913 GAGACG CGTCTC(1/5)
1078 GAGACG
BsrDI 740 GCAATG GCAATG(2/0)
1459 GCAATG
HgaI 511 GCGTC GACGC(5/10)
1308 GCGTC
NlaIV 1239 GGAGCC GG[ACGT]^[ACGT]CC
1280 GGTGCC
RsaI 589 GTAC GT^AC
1657 GTAC
SacI 852 GAGCTC GAGCT^C
1399 GAGCTC
SapI 1647 GAAGAGC GCTCTTC(1/4)
1569 GCTCTTC
Tsp45I 1021 GTCAC ^GT[GC]AC
1310 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AluI AlwI AlwNI AspHI BanII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstNI Cac8I DdeI DpnII EarI
EcoRII EcoT14I Fnu4HI HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MvaI MwoI NdeII NlaIII
PflMI PleI PvuII Sau3AI ScrFI
SfaNI StyI TfiI Tru9I TseI
Tsp509I TspRI Van91I