Restriction map for
pF1KB8574
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# NO CUT ENZYMES ( 133 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BalI BbeI BbrPI BbvCI BcgI
BciVI BcnI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsiEI
BsiWI BsmI BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BssHII
BssSI BstAPI BstBI BstUI CelII
Cfr10I ClaI CpoI DraIII DrdI
DsaI EaeI EagI EclXI Eco47III
Eco52I EcoNI EcoRI EcoRV EcoT22I
FseI FspI HapII Hin1I HpaI
HpaII KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AhdI 1785 GACTAAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2149 CCTCAGG CC^T[ACGT]AGG
AspEI 1785 GACTAAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1591 GAGCTC G[AT]GC[AT]^C
AvaI 1498 CCCGAG C^[CT]CG[GA]G
BamHI 822 GGATCC G^GATCC
BanI 2154 GGCACC G^G[CT][GA]CC
BclI 243 TGATCA T^GATCA
BglI 2086 GCCCGACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsiHKAI 1591 GAGCTC G[AT]GC[AT]^C
BsmBI 1417 CGTCTC CGTCTC(1/5)
BsoBI 1498 CCCGAG C^[CT]CG[GA]G
Bsp1407I 652 TGTACA T^GTACA
BsrGI 652 TGTACA T^GTACA
Bst1107I 794 GTATAC GTA^TAC
BstEII 358 GGTTACC G^GT[ACGT]ACC
BstPI 358 GGTTACC G^GT[ACGT]ACC
BstXI 1196 CCAAACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 794 GTATAC GTA^TAC
Bsu36I 2149 CCTCAGG CC^T[ACGT]AGG
DraI 1090 TTTAAA TTT^AAA
Eam1105I 1785 GACTAAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2149 CCTCAGG CC^T[ACGT]AGG
EcoO65I 358 GGTTACC G^GT[ACGT]ACC
FauI 1290 CCCGC CCCGC(4/6)
FbaI 243 TGATCA T^GATCA
HaeII 1312 AGCGCC [GA]GCGC^[CT]
HgaI 1416 GCGTC GACGC(5/10)
HincII 1793 GTCGAC GT[CT]^[GA]AC
HindII 1793 GTCGAC GT[CT]^[GA]AC
HindIII 809 AAGCTT A^AGCTT
MslI 160 CATCGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflMI 2058 CCACAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 507 GGGACCT [GA]G^G[AT]CC[CT]
SacI 1591 GAGCTC GAGCT^C
SalI 1793 GTCGAC G^TCGAC
SmlI 912 CTCAAG C^T[CT][GA]AG
SspBI 652 TGTACA T^GTACA
Van91I 2058 CCACAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AccI 794 GTATAC GT^[AC][GT]AC
1793 GTCGAC
ApaI 2042 GGGCCC GGGCC^C
2178 GGGCCC
AvaII 508 GGACC G^G[AT]CC
1737 GGTCC
BbsI 1284 GAAGAC GAAGAC(2/6)
26 GTCTTC
BfaI 305 CTAG C^TAG
2293 CTAG
BpuAI 1284 GAAGAC GAAGAC(2/6)
26 GTCTTC
BsmFI 507 GGGAC GGGAC(10/14)
1601 GGGAC
BsrDI 393 CATTGC GCAATG(2/0)
762 CATTGC
CfoI 1313 GCGC GCG^C
1986 GCGC
HhaI 1313 GCGC GCG^C
1986 GCGC
HinP1I 1313 GCGC G^CGC
1986 GCGC
MaeI 305 CTAG C^TAG
2293 CTAG
MaeII 238 ACGT A^CGT
1791 ACGT
MspA1I 563 CAGCTG C[AC]G^C[GT]G
2184 CAGCTG
PvuII 563 CAGCTG CAG^CTG
2184 CAGCTG
SphI 1142 GCATGC GCATG^C
1179 GCATGC
StuI 1610 AGGCCT AGG^CCT
2146 AGGCCT
Tsp45I 77 GTGAC ^GT[GC]AC
890 GTGAC
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI BanII BbvI BmyI
BpmI BsaJI BsgI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeIII MboI
MboII MflI MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PstI RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XhoII