Restriction map for
pF1KB8580
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BamHI BbrPI BbsI BciVI
BclI BfrI BglII BlnI BpmI
BpuAI BsaAI BsaBI BsaI BseAI
BseRI BsiHKAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I ClaI CpoI DraI Eam1105I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KpnI MamI MluI MroI
MslI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccI 713 GTCGAC GT^[AC][GT]AC
AlwNI 860 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 170 AGCGCT AGC^GCT
BalI 335 TGGCCA TGG^CCA
BanI 238 GGCGCC G^G[CT][GA]CC
BbeI 238 GGCGCC GGCGC^C
BcgI 958 CGACCAAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 58 CCGGG CC^[GC]GG
BglI 382 GCCTTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 201 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 201 GCTGAGC GC^T[ACGT]AGC
BsaWI 824 TCCGGT [AT]^CCGG[AT]
BsmI 985 GAATGC GAATGC(1/-1)
CelII 201 GCTGAGC GC^T[ACGT]AGC
DrdI 150 GACCAAGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 65 CCGCGG C^C[GA][CT]GG
EarI 184 CTCTTC CTCTTC(1/4)
Eco47III 170 AGCGCT AGC^GCT
KasI 238 GGCGCC G^GCGCC
Ksp632I 184 CTCTTC CTCTTC(1/4)
KspI 65 CCGCGG CCGC^GG
MfeI 842 CAATTG C^AATTG
MluNI 335 TGGCCA TGG^CCA
MscI 335 TGGCCA TGG^CCA
MunI 842 CAATTG C^AATTG
NaeI 295 GCCGGC GCC^GGC
NarI 238 GGCGCC GG^CGCC
NciI 58 CCGGG CC^[GC]GG
NgoMIV 295 GCCGGC G^CCGGC
NspI 1273 GCATGT [GA]CATG^[CT]
PpuMI 861 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 118 CTGCAG CTGCA^G
PvuII 23 CAGCTG CAG^CTG
SacII 65 CCGCGG CCGC^GG
SalI 713 GTCGAC G^TCGAC
SfoI 238 GGCGCC GGC^GCC
Tsp45I 775 GTGAC ^GT[GC]AC
XbaI 597 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 28 enzymes) #
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AccII 66 CGCG CG^CG
235 CGCG
AfaI 220 GTAC GT^AC
733 GTAC
AvaI 10 CTCGGG C^[CT]CG[GA]G
938 CCCGAG
BbvCI 577 CCTCAGC CCTCAGC(-5/-2)
46 GCTGAGG
BsgI 635 GTGCAG GTGCAG(16/14)
674 GTGCAG
BsiEI 293 CGGCCG CG[GA][CT]^CG
347 CGGCCG
BsoBI 10 CTCGGG C^[CT]CG[GA]G
938 CCCGAG
BspMI 1344 ACCTGC ACCTGC(4/8)
859 GCAGGT
BsrFI 295 GCCGGC [GA]^CCGG[CT]
1186 ACCGGC
BsrI 140 ACTGG ACTGG(1/-1)
222 ACTGG
BstF5I 267 CATCC GGATG(2/0)
624 GGATG
BstUI 66 CGCG CG^CG
235 CGCG
Cfr10I 295 GCCGGC [GA]^CCGG[CT]
1186 ACCGGC
DraIII 494 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
668 CACTCTGTG
EagI 293 CGGCCG C^GGCCG
347 CGGCCG
EclXI 293 CGGCCG C^GGCCG
347 CGGCCG
Eco52I 293 CGGCCG C^GGCCG
347 CGGCCG
EcoT14I 152 CCAAGG C^C[AT][AT]GG
485 CCTTGG
FokI 267 CATCC GGATG(9/13)
624 GGATG
HincII 713 GTCGAC GT[CT]^[GA]AC
925 GTCAAC
HindII 713 GTCGAC GT[CT]^[GA]AC
925 GTCAAC
HphI 88 TCACC GGTGA(8/7)
512 TCACC
MaeII 343 ACGT A^CGT
368 ACGT
MseI 820 TTAA T^TAA
1064 TTAA
MvnI 66 CGCG CG^CG
235 CGCG
RsaI 220 GTAC GT^AC
733 GTAC
StyI 152 CCAAGG C^C[AT][AT]GG
485 CCTTGG
Tru9I 820 TTAA T^TAA
1064 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AcsI AcyI AluI AlwI
ApaI ApoI AvaII BanII BbvI
BfaI BmyI BsaHI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BssKI
BstAPI BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII FauI Fnu4HI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII ItaI MaeI
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TfiI TseI
Tsp509I TspRI TthHB8I XhoII